<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with Wdesk from Workiva-->
<!--Copyright 2021 Workiva-->
<!--r:961af204-71f3-43f3-b200-7cde1263fc2a,g:c06f951d-766b-44ec-b6d7-dd6098524c3c-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/CoverPage" xlink:type="simple" xlink:href="ango-20210831.xsd#CoverPage"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/CoverPage" xlink:type="extended" id="idd5aea40941b432cbddd45a972fb0145_CoverPage">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_7f35f817-1ef9-4e8c-b404-61ed052068ac" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_AmendmentFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_AmendmentFlag_7f35f817-1ef9-4e8c-b404-61ed052068ac" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalYearFocus_9e442825-50a1-4712-86e5-988771c5e035" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentFiscalYearFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_DocumentFiscalYearFocus_9e442825-50a1-4712-86e5-988771c5e035" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalPeriodFocus_4492a8cf-8f91-47a5-9b32-bce98ba03ae6" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentFiscalPeriodFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_DocumentFiscalPeriodFocus_4492a8cf-8f91-47a5-9b32-bce98ba03ae6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_ce943fa6-1b0e-4774-9c1e-e4e045c6f874" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityCentralIndexKey"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_EntityCentralIndexKey_ce943fa6-1b0e-4774-9c1e-e4e045c6f874" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CurrentFiscalYearEndDate_853163c1-4f1c-4c78-ac10-2d55a8d9296b" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_CurrentFiscalYearEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_CurrentFiscalYearEndDate_853163c1-4f1c-4c78-ac10-2d55a8d9296b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_NoTradingSymbolFlag_047b90b7-7460-485a-a57c-b89f7f6ffef2" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_NoTradingSymbolFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_NoTradingSymbolFlag_047b90b7-7460-485a-a57c-b89f7f6ffef2" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_e92fcfd9-b8d2-4966-846d-b1f82d24e4f2" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentType"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_DocumentType_e92fcfd9-b8d2-4966-846d-b1f82d24e4f2" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentQuarterlyReport_e6495e67-73c5-4268-bfb2-0ef7c019c1da" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentQuarterlyReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_DocumentQuarterlyReport_e6495e67-73c5-4268-bfb2-0ef7c019c1da" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentPeriodEndDate_5c0f5a68-b236-47e4-9dc6-87add2f9b799" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentPeriodEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_DocumentPeriodEndDate_5c0f5a68-b236-47e4-9dc6-87add2f9b799" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentTransitionReport_7ddc5cbf-c86a-499f-a554-006f06278d0f" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentTransitionReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_DocumentTransitionReport_7ddc5cbf-c86a-499f-a554-006f06278d0f" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_ddc67434-d391-4288-bd0c-13cc4592b12c" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityFileNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_EntityFileNumber_ddc67434-d391-4288-bd0c-13cc4592b12c" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_a5aef7d9-458c-4f20-882a-c45ddd64dce2" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityRegistrantName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_EntityRegistrantName_a5aef7d9-458c-4f20-882a-c45ddd64dce2" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_f7cf085f-e276-48e8-ab94-cf35ef2a9dca" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_EntityIncorporationStateCountryCode_f7cf085f-e276-48e8-ab94-cf35ef2a9dca" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_900c161f-c9c0-4f12-a178-93afaa7c0dab" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_EntityTaxIdentificationNumber_900c161f-c9c0-4f12-a178-93afaa7c0dab" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_7f6b5747-2602-4c7d-b9f0-4145e4b145b1" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityAddressAddressLine1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_EntityAddressAddressLine1_7f6b5747-2602-4c7d-b9f0-4145e4b145b1" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_427f1cd6-d712-4789-8e66-81b370567b31" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityAddressCityOrTown"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_EntityAddressCityOrTown_427f1cd6-d712-4789-8e66-81b370567b31" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressStateOrProvince_ce25b777-99de-4ecf-898f-b88e6a528fe0" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityAddressStateOrProvince"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_EntityAddressStateOrProvince_ce25b777-99de-4ecf-898f-b88e6a528fe0" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_83729559-0e77-40ad-a628-2de3757bf0bc" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityAddressPostalZipCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_EntityAddressPostalZipCode_83729559-0e77-40ad-a628-2de3757bf0bc" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_1c3c2b78-648c-40bf-bc2f-f5f82a872a9d" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_CityAreaCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_CityAreaCode_1c3c2b78-648c-40bf-bc2f-f5f82a872a9d" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_7d029846-3f88-41e2-9d70-d1c33dd9bd44" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_LocalPhoneNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_LocalPhoneNumber_7d029846-3f88-41e2-9d70-d1c33dd9bd44" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_014b1451-1b37-4599-848e-e602e0d14f63" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_Security12bTitle"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_Security12bTitle_014b1451-1b37-4599-848e-e602e0d14f63" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_bb00340e-c7f7-4503-908e-fb0efff56214" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_TradingSymbol"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_TradingSymbol_bb00340e-c7f7-4503-908e-fb0efff56214" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_5955c476-9cc0-4a55-a4e7-b953429cba28" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_SecurityExchangeName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_SecurityExchangeName_5955c476-9cc0-4a55-a4e7-b953429cba28" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCurrentReportingStatus_332c8c83-83fa-4af5-88e8-b5b615de1513" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityCurrentReportingStatus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_EntityCurrentReportingStatus_332c8c83-83fa-4af5-88e8-b5b615de1513" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityInteractiveDataCurrent_e8c7760a-0eec-47b3-9516-689c6b53a599" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityInteractiveDataCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_EntityInteractiveDataCurrent_e8c7760a-0eec-47b3-9516-689c6b53a599" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFilerCategory_38202c00-e0e8-4fde-accb-449e84128a1a" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityFilerCategory"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_EntityFilerCategory_38202c00-e0e8-4fde-accb-449e84128a1a" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntitySmallBusiness_a9e0f7ee-c480-4446-bb9d-139dab68824b" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntitySmallBusiness"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_EntitySmallBusiness_a9e0f7ee-c480-4446-bb9d-139dab68824b" xlink:type="arc" order="26"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_a298a5c6-1f5f-47f6-a3c8-75be11ec4c60" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_EntityEmergingGrowthCompany_a298a5c6-1f5f-47f6-a3c8-75be11ec4c60" xlink:type="arc" order="27"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityShellCompany_94f2aaf0-a84d-4295-a83e-41515fc1aa1e" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityShellCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_EntityShellCompany_94f2aaf0-a84d-4295-a83e-41515fc1aa1e" xlink:type="arc" order="28"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCommonStockSharesOutstanding_fd8852d6-e4fd-4a76-bcf6-a76adb9190cf" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityCommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_dei_EntityCommonStockSharesOutstanding_fd8852d6-e4fd-4a76-bcf6-a76adb9190cf" xlink:type="arc" order="29"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_ae075db9-ed9c-4ac7-8912-e86bc6e737a7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_e7c88bfe-896f-4b8d-b728-66730f4c8130" xlink:to="loc_us-gaap_StatementTable_ae075db9-ed9c-4ac7-8912-e86bc6e737a7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_69d98c99-1ebd-4428-aed7-29a3106aa5ac" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_ae075db9-ed9c-4ac7-8912-e86bc6e737a7" xlink:to="loc_us-gaap_StatementClassOfStockAxis_69d98c99-1ebd-4428-aed7-29a3106aa5ac" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_69d98c99-1ebd-4428-aed7-29a3106aa5ac_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_69d98c99-1ebd-4428-aed7-29a3106aa5ac" xlink:to="loc_us-gaap_ClassOfStockDomain_69d98c99-1ebd-4428-aed7-29a3106aa5ac_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_80bc5bd4-6c50-4570-81e1-83f7bc9c11c2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_69d98c99-1ebd-4428-aed7-29a3106aa5ac" xlink:to="loc_us-gaap_ClassOfStockDomain_80bc5bd4-6c50-4570-81e1-83f7bc9c11c2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_0d79cbe9-2a6a-469e-971c-488c903f5baa" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_80bc5bd4-6c50-4570-81e1-83f7bc9c11c2" xlink:to="loc_us-gaap_CommonStockMember_0d79cbe9-2a6a-469e-971c-488c903f5baa" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_PreferredStockPurchaseRightsMember_141dc1e8-654a-4158-a70d-3889df01fab9" xlink:href="ango-20210831.xsd#ango_PreferredStockPurchaseRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_80bc5bd4-6c50-4570-81e1-83f7bc9c11c2" xlink:to="loc_ango_PreferredStockPurchaseRightsMember_141dc1e8-654a-4158-a70d-3889df01fab9" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/ConsolidatedStatementsofStockholdersEquity" xlink:type="simple" xlink:href="ango-20210831.xsd#ConsolidatedStatementsofStockholdersEquity"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/ConsolidatedStatementsofStockholdersEquity" xlink:type="extended" id="i75a556ffd64b44cabe4a85c3619c1b3d_ConsolidatedStatementsofStockholdersEquity">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_7e3738f0-a1d3-43cd-8861-e69a9f0e6bdc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_989359d2-e1ec-4ac3-8115-c0e5f560a99d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncreaseDecreaseInStockholdersEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_7e3738f0-a1d3-43cd-8861-e69a9f0e6bdc" xlink:to="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_989359d2-e1ec-4ac3-8115-c0e5f560a99d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesIssued_1da34a86-a278-411b-9fca-4f9955009a54" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_989359d2-e1ec-4ac3-8115-c0e5f560a99d" xlink:to="loc_us-gaap_SharesIssued_1da34a86-a278-411b-9fca-4f9955009a54" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_614b1924-b9dc-46b2-9378-07a7284953d2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_989359d2-e1ec-4ac3-8115-c0e5f560a99d" xlink:to="loc_us-gaap_StockholdersEquity_614b1924-b9dc-46b2-9378-07a7284953d2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockShares_9c65ef13-e367-4fb5-9d65-59b3f4391fa1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TreasuryStockShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_989359d2-e1ec-4ac3-8115-c0e5f560a99d" xlink:to="loc_us-gaap_TreasuryStockShares_9c65ef13-e367-4fb5-9d65-59b3f4391fa1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_c0c6c262-3f93-4fe5-a04e-176203668fba" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_989359d2-e1ec-4ac3-8115-c0e5f560a99d" xlink:to="loc_us-gaap_NetIncomeLoss_c0c6c262-3f93-4fe5-a04e-176203668fba" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_d4f983e5-5b01-424c-aa24-da9fa2d3013a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_989359d2-e1ec-4ac3-8115-c0e5f560a99d" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_d4f983e5-5b01-424c-aa24-da9fa2d3013a" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_2d713637-70d3-4fdf-ac8f-dbad051d62c2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_989359d2-e1ec-4ac3-8115-c0e5f560a99d" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_2d713637-70d3-4fdf-ac8f-dbad051d62c2" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation_19b9fbe9-e6b5-44a9-81e0-57d057d010ec" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_989359d2-e1ec-4ac3-8115-c0e5f560a99d" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation_19b9fbe9-e6b5-44a9-81e0-57d057d010ec" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation_925a72d9-5146-43f3-97ef-230669ac8da5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_989359d2-e1ec-4ac3-8115-c0e5f560a99d" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation_925a72d9-5146-43f3-97ef-230669ac8da5" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans_e061e271-b367-4bd5-a596-24fa1c2e1ccb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_989359d2-e1ec-4ac3-8115-c0e5f560a99d" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans_e061e271-b367-4bd5-a596-24fa1c2e1ccb" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan_acce43c9-582a-444a-972c-2d92a95abe67" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_989359d2-e1ec-4ac3-8115-c0e5f560a99d" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan_acce43c9-582a-444a-972c-2d92a95abe67" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_f0606aa9-8fad-4ef7-a599-a731e24041dc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_989359d2-e1ec-4ac3-8115-c0e5f560a99d" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_f0606aa9-8fad-4ef7-a599-a731e24041dc" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_015a25c5-b740-40a5-9eb6-6bd3fa4601ed" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_989359d2-e1ec-4ac3-8115-c0e5f560a99d" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_015a25c5-b740-40a5-9eb6-6bd3fa4601ed" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesIssued_7cbead2a-25f0-403a-bad4-ad5b92ea0792" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharesIssued"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_7febed3c-743d-4ce8-abdc-e664c184eeb7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockShares_d5b7cb36-7487-41e0-9b18-56146fee6267" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TreasuryStockShares"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_428b9490-1b21-4c0c-ab27-0f2553b94e0a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_7e3738f0-a1d3-43cd-8861-e69a9f0e6bdc" xlink:to="loc_us-gaap_StatementTable_428b9490-1b21-4c0c-ab27-0f2553b94e0a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_9c8210a0-c877-4e14-add2-4274d0bab4f6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_428b9490-1b21-4c0c-ab27-0f2553b94e0a" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_9c8210a0-c877-4e14-add2-4274d0bab4f6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_9c8210a0-c877-4e14-add2-4274d0bab4f6_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_9c8210a0-c877-4e14-add2-4274d0bab4f6" xlink:to="loc_us-gaap_EquityComponentDomain_9c8210a0-c877-4e14-add2-4274d0bab4f6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_367466c2-837c-4489-906a-3fed92ad8581" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_9c8210a0-c877-4e14-add2-4274d0bab4f6" xlink:to="loc_us-gaap_EquityComponentDomain_367466c2-837c-4489-906a-3fed92ad8581" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_f26686a0-8668-4920-96e7-c16964396970" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_367466c2-837c-4489-906a-3fed92ad8581" xlink:to="loc_us-gaap_CommonStockMember_f26686a0-8668-4920-96e7-c16964396970" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_25c6f90f-7221-4898-b70a-290ec5235952" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_367466c2-837c-4489-906a-3fed92ad8581" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_25c6f90f-7221-4898-b70a-290ec5235952" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_6bbf6568-c392-4fc5-9553-941ba87c115d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_367466c2-837c-4489-906a-3fed92ad8581" xlink:to="loc_us-gaap_RetainedEarningsMember_6bbf6568-c392-4fc5-9553-941ba87c115d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_eb73c2d5-f613-4ca5-ade7-6f23eb4ba569" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_367466c2-837c-4489-906a-3fed92ad8581" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_eb73c2d5-f613-4ca5-ade7-6f23eb4ba569" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockMember_1145adc0-15dc-4876-8da4-8d362521dec8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TreasuryStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_367466c2-837c-4489-906a-3fed92ad8581" xlink:to="loc_us-gaap_TreasuryStockMember_1145adc0-15dc-4876-8da4-8d362521dec8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_824b241a-532b-4628-a639-86ec11a90381" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_428b9490-1b21-4c0c-ab27-0f2553b94e0a" xlink:to="loc_us-gaap_AwardTypeAxis_824b241a-532b-4628-a639-86ec11a90381" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_824b241a-532b-4628-a639-86ec11a90381_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_824b241a-532b-4628-a639-86ec11a90381" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_824b241a-532b-4628-a639-86ec11a90381_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_74cf1297-d842-4dbb-92e9-b605f87cad5b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_824b241a-532b-4628-a639-86ec11a90381" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_74cf1297-d842-4dbb-92e9-b605f87cad5b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_ed34a54c-580c-4e18-b8e9-33c9655ff679" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_74cf1297-d842-4dbb-92e9-b605f87cad5b" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_ed34a54c-580c-4e18-b8e9-33c9655ff679" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AcquisitionsDetails" xlink:type="simple" xlink:href="ango-20210831.xsd#AcquisitionsDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AcquisitionsDetails" xlink:type="extended" id="i4341955df9f749ebb710b350a56dfb50_AcquisitionsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionLineItems_483e89ac-ae70-400a-beab-d5e3523bd9d5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_AssetAcquisitionConsiderationTransferred_e362d21c-8f92-4791-a9fb-1d1b9e0c1f2b" xlink:href="ango-20210831.xsd#ango_AssetAcquisitionConsiderationTransferred"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_483e89ac-ae70-400a-beab-d5e3523bd9d5" xlink:to="loc_ango_AssetAcquisitionConsiderationTransferred_e362d21c-8f92-4791-a9fb-1d1b9e0c1f2b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiability_11145cf5-c3c4-4fce-8d79-f948edb1b8ca" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContractWithCustomerLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_483e89ac-ae70-400a-beab-d5e3523bd9d5" xlink:to="loc_us-gaap_ContractWithCustomerLiability_11145cf5-c3c4-4fce-8d79-f948edb1b8ca" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityCurrent_4153a29c-4060-40da-9b46-492999503c08" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContractWithCustomerLiabilityCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_483e89ac-ae70-400a-beab-d5e3523bd9d5" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityCurrent_4153a29c-4060-40da-9b46-492999503c08" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_RevenueRemainingPerformanceObligationVariableConsiderationAmount_3a7cf428-962d-4402-bb32-5fb16298c82e" xlink:href="ango-20210831.xsd#ango_RevenueRemainingPerformanceObligationVariableConsiderationAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_483e89ac-ae70-400a-beab-d5e3523bd9d5" xlink:to="loc_ango_RevenueRemainingPerformanceObligationVariableConsiderationAmount_3a7cf428-962d-4402-bb32-5fb16298c82e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_AssetAcquisitionInventoryAndFixedAssets_70fddd31-2607-4933-a7e7-ea14735d3703" xlink:href="ango-20210831.xsd#ango_AssetAcquisitionInventoryAndFixedAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_483e89ac-ae70-400a-beab-d5e3523bd9d5" xlink:to="loc_ango_AssetAcquisitionInventoryAndFixedAssets_70fddd31-2607-4933-a7e7-ea14735d3703" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinitelivedIntangibleAssetsAcquired1_507f40c5-2df7-4f69-83bd-a5fdfe3e7a92" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FinitelivedIntangibleAssetsAcquired1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_483e89ac-ae70-400a-beab-d5e3523bd9d5" xlink:to="loc_us-gaap_FinitelivedIntangibleAssetsAcquired1_507f40c5-2df7-4f69-83bd-a5fdfe3e7a92" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsRemainingAmortizationPeriod1_0f070000-750d-4b14-878d-236e53bd3aaa" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsRemainingAmortizationPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_483e89ac-ae70-400a-beab-d5e3523bd9d5" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsRemainingAmortizationPeriod1_0f070000-750d-4b14-878d-236e53bd3aaa" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_88d8693f-6561-47ac-8e4c-bda8e9ae7531" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_483e89ac-ae70-400a-beab-d5e3523bd9d5" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_88d8693f-6561-47ac-8e4c-bda8e9ae7531" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_7af69003-c9c4-4f4e-861c-1c8b9f751bad" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_88d8693f-6561-47ac-8e4c-bda8e9ae7531" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_7af69003-c9c4-4f4e-861c-1c8b9f751bad" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_7af69003-c9c4-4f4e-861c-1c8b9f751bad_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_7af69003-c9c4-4f4e-861c-1c8b9f751bad" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_7af69003-c9c4-4f4e-861c-1c8b9f751bad_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_4dcd8f90-7f40-4ac5-9c91-72d8f13533ed" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_7af69003-c9c4-4f4e-861c-1c8b9f751bad" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_4dcd8f90-7f40-4ac5-9c91-72d8f13533ed" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TechnologyBasedIntangibleAssetsMember_f8dea486-bcb8-4688-8549-bbbd84ce6ea0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TechnologyBasedIntangibleAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_4dcd8f90-7f40-4ac5-9c91-72d8f13533ed" xlink:to="loc_us-gaap_TechnologyBasedIntangibleAssetsMember_f8dea486-bcb8-4688-8549-bbbd84ce6ea0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_AssetAcquisitionAxis_b19eb74f-62fb-4bed-9765-a73f1b5b9f71" xlink:href="ango-20210831.xsd#ango_AssetAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_88d8693f-6561-47ac-8e4c-bda8e9ae7531" xlink:to="loc_ango_AssetAcquisitionAxis_b19eb74f-62fb-4bed-9765-a73f1b5b9f71" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_AssetAcquisitionDomain_b19eb74f-62fb-4bed-9765-a73f1b5b9f71_default" xlink:href="ango-20210831.xsd#ango_AssetAcquisitionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ango_AssetAcquisitionAxis_b19eb74f-62fb-4bed-9765-a73f1b5b9f71" xlink:to="loc_ango_AssetAcquisitionDomain_b19eb74f-62fb-4bed-9765-a73f1b5b9f71_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_AssetAcquisitionDomain_d7470a87-133b-414b-ae3a-2b330f5be78c" xlink:href="ango-20210831.xsd#ango_AssetAcquisitionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ango_AssetAcquisitionAxis_b19eb74f-62fb-4bed-9765-a73f1b5b9f71" xlink:to="loc_ango_AssetAcquisitionDomain_d7470a87-133b-414b-ae3a-2b330f5be78c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_QXMedicalLLCCamaroSupportCatheterAssetAcquisitionMember_e5abc260-c862-4881-9b87-b81722f64253" xlink:href="ango-20210831.xsd#ango_QXMedicalLLCCamaroSupportCatheterAssetAcquisitionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ango_AssetAcquisitionDomain_d7470a87-133b-414b-ae3a-2b330f5be78c" xlink:to="loc_ango_QXMedicalLLCCamaroSupportCatheterAssetAcquisitionMember_e5abc260-c862-4881-9b87-b81722f64253" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/RevenuefromContractswithCustomersDisaggregationofRevenueDetails" xlink:type="simple" xlink:href="ango-20210831.xsd#RevenuefromContractswithCustomersDisaggregationofRevenueDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/RevenuefromContractswithCustomersDisaggregationofRevenueDetails" xlink:type="extended" id="i9b9ea27a0e184d348e6ee11913adbd91_RevenuefromContractswithCustomersDisaggregationofRevenueDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_41a64336-cdd2-45b3-92e1-9c3244fc12ed" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_9f9e11d4-ab78-4b1f-941d-009e041e27a1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_41a64336-cdd2-45b3-92e1-9c3244fc12ed" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_9f9e11d4-ab78-4b1f-941d-009e041e27a1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueTable_fd0e1bbf-e4dd-4fcf-bfd1-4a8be47444a8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DisaggregationOfRevenueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_41a64336-cdd2-45b3-92e1-9c3244fc12ed" xlink:to="loc_us-gaap_DisaggregationOfRevenueTable_fd0e1bbf-e4dd-4fcf-bfd1-4a8be47444a8" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_8e8d6750-1808-4fe8-bdc6-f33937a3716f" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_fd0e1bbf-e4dd-4fcf-bfd1-4a8be47444a8" xlink:to="loc_srt_ProductOrServiceAxis_8e8d6750-1808-4fe8-bdc6-f33937a3716f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_8e8d6750-1808-4fe8-bdc6-f33937a3716f_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_8e8d6750-1808-4fe8-bdc6-f33937a3716f" xlink:to="loc_srt_ProductsAndServicesDomain_8e8d6750-1808-4fe8-bdc6-f33937a3716f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_b8706f9a-f341-446f-affb-ca5e3c88137d" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_8e8d6750-1808-4fe8-bdc6-f33937a3716f" xlink:to="loc_srt_ProductsAndServicesDomain_b8706f9a-f341-446f-affb-ca5e3c88137d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_EndovascularTherapiesMember_66daf138-eb0d-4540-bf71-99c4819052f9" xlink:href="ango-20210831.xsd#ango_EndovascularTherapiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_b8706f9a-f341-446f-affb-ca5e3c88137d" xlink:to="loc_ango_EndovascularTherapiesMember_66daf138-eb0d-4540-bf71-99c4819052f9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_VascularAccessMember_d1c9ac02-3e6b-4d11-93e6-7cd33e4f6f29" xlink:href="ango-20210831.xsd#ango_VascularAccessMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_b8706f9a-f341-446f-affb-ca5e3c88137d" xlink:to="loc_ango_VascularAccessMember_d1c9ac02-3e6b-4d11-93e6-7cd33e4f6f29" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_OncologyMember_474c24f2-2677-4c8f-a770-45e1adbf854a" xlink:href="ango-20210831.xsd#ango_OncologyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_b8706f9a-f341-446f-affb-ca5e3c88137d" xlink:to="loc_ango_OncologyMember_474c24f2-2677-4c8f-a770-45e1adbf854a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_MedTechMember_4675cb68-c3ec-4693-9da4-45b7bd3f6cb0" xlink:href="ango-20210831.xsd#ango_MedTechMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_b8706f9a-f341-446f-affb-ca5e3c88137d" xlink:to="loc_ango_MedTechMember_4675cb68-c3ec-4693-9da4-45b7bd3f6cb0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_MedDeviceMember_db0bc797-e5f3-4f28-8ae7-4b2890284773" xlink:href="ango-20210831.xsd#ango_MedDeviceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_b8706f9a-f341-446f-affb-ca5e3c88137d" xlink:to="loc_ango_MedDeviceMember_db0bc797-e5f3-4f28-8ae7-4b2890284773" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_e56d7d59-e6d6-4c5e-a1fd-4af980f1039b" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_fd0e1bbf-e4dd-4fcf-bfd1-4a8be47444a8" xlink:to="loc_srt_StatementGeographicalAxis_e56d7d59-e6d6-4c5e-a1fd-4af980f1039b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_e56d7d59-e6d6-4c5e-a1fd-4af980f1039b_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_e56d7d59-e6d6-4c5e-a1fd-4af980f1039b" xlink:to="loc_srt_SegmentGeographicalDomain_e56d7d59-e6d6-4c5e-a1fd-4af980f1039b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_ab9025d8-88c3-49cb-852d-fa11c45b963d" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_e56d7d59-e6d6-4c5e-a1fd-4af980f1039b" xlink:to="loc_srt_SegmentGeographicalDomain_ab9025d8-88c3-49cb-852d-fa11c45b963d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_cacd2f96-7533-468e-a852-2c2295e3f4ad" xlink:href="https://xbrl.sec.gov/country/2020/country-2020-01-31.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_ab9025d8-88c3-49cb-852d-fa11c45b963d" xlink:to="loc_country_US_cacd2f96-7533-468e-a852-2c2295e3f4ad" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonUsMember_bcf67d7a-952a-4926-a8be-5507178f27ee" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NonUsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_ab9025d8-88c3-49cb-852d-fa11c45b963d" xlink:to="loc_us-gaap_NonUsMember_bcf67d7a-952a-4926-a8be-5507178f27ee" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/RevenuefromContractswithCustomersNarrativeDetails" xlink:type="simple" xlink:href="ango-20210831.xsd#RevenuefromContractswithCustomersNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/RevenuefromContractswithCustomersNarrativeDetails" xlink:type="extended" id="i4738b582bfb54ded916d14b9b92aeca4_RevenuefromContractswithCustomersNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_2995f46d-44cd-419a-b384-8385352795c5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_RevenuePerformanceObligationPaymentTerm_13965ace-f74a-4a8f-b177-85b74c600d62" xlink:href="ango-20210831.xsd#ango_RevenuePerformanceObligationPaymentTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_2995f46d-44cd-419a-b384-8385352795c5" xlink:to="loc_ango_RevenuePerformanceObligationPaymentTerm_13965ace-f74a-4a8f-b177-85b74c600d62" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_ContractWithCustomerRightOfReturnProductReturnPeriodAfterPurchaseDateRequiringPreApproval_ca0b34be-d2ad-494b-9c26-61a52e4c8ffc" xlink:href="ango-20210831.xsd#ango_ContractWithCustomerRightOfReturnProductReturnPeriodAfterPurchaseDateRequiringPreApproval"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_2995f46d-44cd-419a-b384-8385352795c5" xlink:to="loc_ango_ContractWithCustomerRightOfReturnProductReturnPeriodAfterPurchaseDateRequiringPreApproval_ca0b34be-d2ad-494b-9c26-61a52e4c8ffc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_ContractwithCustomerRightofReturnRestockingCharge_42992358-efa8-477f-a852-987a1d6c01d2" xlink:href="ango-20210831.xsd#ango_ContractwithCustomerRightofReturnRestockingCharge"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_2995f46d-44cd-419a-b384-8385352795c5" xlink:to="loc_ango_ContractwithCustomerRightofReturnRestockingCharge_42992358-efa8-477f-a852-987a1d6c01d2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_ContractWithCustomerRightOfReturnMinimumRemainingProductLifePriorToReturn_02965a03-9a5c-4d1c-b2e6-546a8db2d5fd" xlink:href="ango-20210831.xsd#ango_ContractWithCustomerRightOfReturnMinimumRemainingProductLifePriorToReturn"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_2995f46d-44cd-419a-b384-8385352795c5" xlink:to="loc_ango_ContractWithCustomerRightOfReturnMinimumRemainingProductLifePriorToReturn_02965a03-9a5c-4d1c-b2e6-546a8db2d5fd" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityIncreaseDecreaseForContractAcquiredInBusinessCombination_b2bc6752-9848-4435-aecf-f1216a2f5d52" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContractWithCustomerLiabilityIncreaseDecreaseForContractAcquiredInBusinessCombination"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_2995f46d-44cd-419a-b384-8385352795c5" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityIncreaseDecreaseForContractAcquiredInBusinessCombination_b2bc6752-9848-4435-aecf-f1216a2f5d52" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityRevenueRecognized_aca8cdac-d599-4b7d-9640-c525f8830adc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContractWithCustomerLiabilityRevenueRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_2995f46d-44cd-419a-b384-8385352795c5" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityRevenueRecognized_aca8cdac-d599-4b7d-9640-c525f8830adc" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueTable_5a26a4a0-c26a-45dd-980d-5a3dd5b16c78" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DisaggregationOfRevenueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_2995f46d-44cd-419a-b384-8385352795c5" xlink:to="loc_us-gaap_DisaggregationOfRevenueTable_5a26a4a0-c26a-45dd-980d-5a3dd5b16c78" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_dd72bb0e-cd7d-4938-91da-3b1ad1d71549" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_5a26a4a0-c26a-45dd-980d-5a3dd5b16c78" xlink:to="loc_srt_RangeAxis_dd72bb0e-cd7d-4938-91da-3b1ad1d71549" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_dd72bb0e-cd7d-4938-91da-3b1ad1d71549_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_dd72bb0e-cd7d-4938-91da-3b1ad1d71549" xlink:to="loc_srt_RangeMember_dd72bb0e-cd7d-4938-91da-3b1ad1d71549_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_a34da912-6435-4330-914f-24bac9ee2385" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_dd72bb0e-cd7d-4938-91da-3b1ad1d71549" xlink:to="loc_srt_RangeMember_a34da912-6435-4330-914f-24bac9ee2385" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_ef38c933-e89e-4599-91a0-32947b91d02c" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_a34da912-6435-4330-914f-24bac9ee2385" xlink:to="loc_srt_MinimumMember_ef38c933-e89e-4599-91a0-32947b91d02c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_d50e9169-d1a0-442b-a65e-3e88f7fa6d83" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_a34da912-6435-4330-914f-24bac9ee2385" xlink:to="loc_srt_MaximumMember_d50e9169-d1a0-442b-a65e-3e88f7fa6d83" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/GoodwillandIntangibleAssetsNarrativeDetails" xlink:type="simple" xlink:href="ango-20210831.xsd#GoodwillandIntangibleAssetsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/GoodwillandIntangibleAssetsNarrativeDetails" xlink:type="extended" id="i5343cc8b7ca64ac286091c71a2b5575f_GoodwillandIntangibleAssetsNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_0881283d-176a-43f7-948d-7bb45ae2544d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfReportingUnits_7eb7291e-851e-466d-bd5c-014c3d8f6dad" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NumberOfReportingUnits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_0881283d-176a-43f7-948d-7bb45ae2544d" xlink:to="loc_us-gaap_NumberOfReportingUnits_7eb7291e-851e-466d-bd5c-014c3d8f6dad" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillImpairmentLoss_fa54902f-a594-413c-8414-ed60e6ef7eb1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GoodwillImpairmentLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_0881283d-176a-43f7-948d-7bb45ae2544d" xlink:to="loc_us-gaap_GoodwillImpairmentLoss_fa54902f-a594-413c-8414-ed60e6ef7eb1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_1ed5c6e5-62fa-4fe4-84c5-3c228c61b03f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_0881283d-176a-43f7-948d-7bb45ae2544d" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_1ed5c6e5-62fa-4fe4-84c5-3c228c61b03f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfIntangibleAssets_ef459afc-e685-4cdf-86a9-9961c3a85b5a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AmortizationOfIntangibleAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_0881283d-176a-43f7-948d-7bb45ae2544d" xlink:to="loc_us-gaap_AmortizationOfIntangibleAssets_ef459afc-e685-4cdf-86a9-9961c3a85b5a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_915cc0a9-9fd8-460c-82dd-e8f7c9cabb2d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_0881283d-176a-43f7-948d-7bb45ae2544d" xlink:to="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_915cc0a9-9fd8-460c-82dd-e8f7c9cabb2d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_b08bbd80-b820-4c19-8b54-bf0906236b09" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_915cc0a9-9fd8-460c-82dd-e8f7c9cabb2d" xlink:to="loc_srt_RangeAxis_b08bbd80-b820-4c19-8b54-bf0906236b09" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_b08bbd80-b820-4c19-8b54-bf0906236b09_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_b08bbd80-b820-4c19-8b54-bf0906236b09" xlink:to="loc_srt_RangeMember_b08bbd80-b820-4c19-8b54-bf0906236b09_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_9934dec1-2dbc-474d-9e91-76322435946a" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_b08bbd80-b820-4c19-8b54-bf0906236b09" xlink:to="loc_srt_RangeMember_9934dec1-2dbc-474d-9e91-76322435946a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_28b30930-0d74-405c-8b55-685672b11e44" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_9934dec1-2dbc-474d-9e91-76322435946a" xlink:to="loc_srt_MinimumMember_28b30930-0d74-405c-8b55-685672b11e44" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_337e2c8d-bcb7-4c20-87a8-1ae08df92c9c" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_9934dec1-2dbc-474d-9e91-76322435946a" xlink:to="loc_srt_MaximumMember_337e2c8d-bcb7-4c20-87a8-1ae08df92c9c" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/GoodwillandIntangibleAssetsIntangibleAssetsDetails" xlink:type="simple" xlink:href="ango-20210831.xsd#GoodwillandIntangibleAssetsIntangibleAssetsDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/GoodwillandIntangibleAssetsIntangibleAssetsDetails" xlink:type="extended" id="ic02725bf0dcb41b3bcb493f4b9caf9f6_GoodwillandIntangibleAssetsIntangibleAssetsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_d397e388-2c21-4c44-af60-9571d3c1c32a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_72f6c3c9-b0a6-4dfa-b266-3b7f1016c358" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_d397e388-2c21-4c44-af60-9571d3c1c32a" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_72f6c3c9-b0a6-4dfa-b266-3b7f1016c358" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_2a215119-4ff1-4ec5-8008-588103dfa374" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_d397e388-2c21-4c44-af60-9571d3c1c32a" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_2a215119-4ff1-4ec5-8008-588103dfa374" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_43f59ad9-e259-44f3-9070-a7b9068dada8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_d397e388-2c21-4c44-af60-9571d3c1c32a" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNet_43f59ad9-e259-44f3-9070-a7b9068dada8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_31164c47-4d70-4e30-841b-474a0d4155a8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_d397e388-2c21-4c44-af60-9571d3c1c32a" xlink:to="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_31164c47-4d70-4e30-841b-474a0d4155a8" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_525dbd4a-9f49-4c5e-8d44-1caa539d3002" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_31164c47-4d70-4e30-841b-474a0d4155a8" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_525dbd4a-9f49-4c5e-8d44-1caa539d3002" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_525dbd4a-9f49-4c5e-8d44-1caa539d3002_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_525dbd4a-9f49-4c5e-8d44-1caa539d3002" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_525dbd4a-9f49-4c5e-8d44-1caa539d3002_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_e98bd634-0eca-4446-86c1-1f0821a85fd5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_525dbd4a-9f49-4c5e-8d44-1caa539d3002" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_e98bd634-0eca-4446-86c1-1f0821a85fd5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_ProductTechnologiesMember_c4f19b96-ec85-4559-8805-b7129cecad7d" xlink:href="ango-20210831.xsd#ango_ProductTechnologiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_e98bd634-0eca-4446-86c1-1f0821a85fd5" xlink:to="loc_ango_ProductTechnologiesMember_c4f19b96-ec85-4559-8805-b7129cecad7d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerRelationshipsMember_b85d341d-e07c-4132-b385-980a93149ccd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CustomerRelationshipsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_e98bd634-0eca-4446-86c1-1f0821a85fd5" xlink:to="loc_us-gaap_CustomerRelationshipsMember_b85d341d-e07c-4132-b385-980a93149ccd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TrademarksMember_6a55bea5-716d-4d7f-8e72-b216ee1cb482" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TrademarksMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_e98bd634-0eca-4446-86c1-1f0821a85fd5" xlink:to="loc_us-gaap_TrademarksMember_6a55bea5-716d-4d7f-8e72-b216ee1cb482" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LicenseAgreementTermsMember_876e1c96-ce4b-4de9-9883-ddcdad29afd9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LicenseAgreementTermsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_e98bd634-0eca-4446-86c1-1f0821a85fd5" xlink:to="loc_us-gaap_LicenseAgreementTermsMember_876e1c96-ce4b-4de9-9883-ddcdad29afd9" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/LongTermDebtDetails" xlink:type="simple" xlink:href="ango-20210831.xsd#LongTermDebtDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/LongTermDebtDetails" xlink:type="extended" id="i4dfefc344da141e68f13f1900afe3f31_LongTermDebtDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_b0a3ec03-b3c0-42c3-8de4-4aa0a7146dd8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_e83e453b-2d87-45d3-936e-20454ceab62a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b0a3ec03-b3c0-42c3-8de4-4aa0a7146dd8" xlink:to="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_e83e453b-2d87-45d3-936e-20454ceab62a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_LineOfCreditFacilityMaximumBorrowingCapacityAccordionFeature_653cbe7c-c39b-4a85-a066-140d51c36a67" xlink:href="ango-20210831.xsd#ango_LineOfCreditFacilityMaximumBorrowingCapacityAccordionFeature"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b0a3ec03-b3c0-42c3-8de4-4aa0a7146dd8" xlink:to="loc_ango_LineOfCreditFacilityMaximumBorrowingCapacityAccordionFeature_653cbe7c-c39b-4a85-a066-140d51c36a67" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTerm_981d69c1-ee53-45cd-bab4-d7419ff63f09" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b0a3ec03-b3c0-42c3-8de4-4aa0a7146dd8" xlink:to="loc_us-gaap_DebtInstrumentTerm_981d69c1-ee53-45cd-bab4-d7419ff63f09" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_e1658e02-1c92-408b-85ab-d5aa30a20ab0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b0a3ec03-b3c0-42c3-8de4-4aa0a7146dd8" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_e1658e02-1c92-408b-85ab-d5aa30a20ab0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_DebtInstrumentDebtDefaultInterestRateIncrease_751d7910-dac9-4d1e-a632-7916eb611b82" xlink:href="ango-20210831.xsd#ango_DebtInstrumentDebtDefaultInterestRateIncrease"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b0a3ec03-b3c0-42c3-8de4-4aa0a7146dd8" xlink:to="loc_ango_DebtInstrumentDebtDefaultInterestRateIncrease_751d7910-dac9-4d1e-a632-7916eb611b82" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityCommitmentFeePercentage_e8e31fd6-6f00-4a5a-b0f9-ac2176cbe038" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityCommitmentFeePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b0a3ec03-b3c0-42c3-8de4-4aa0a7146dd8" xlink:to="loc_us-gaap_LineOfCreditFacilityCommitmentFeePercentage_e8e31fd6-6f00-4a5a-b0f9-ac2176cbe038" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_LineOfCreditFacilityNumberOfCovenants_a24b955c-800f-405b-813d-7284b0c30a70" xlink:href="ango-20210831.xsd#ango_LineOfCreditFacilityNumberOfCovenants"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b0a3ec03-b3c0-42c3-8de4-4aa0a7146dd8" xlink:to="loc_ango_LineOfCreditFacilityNumberOfCovenants_a24b955c-800f-405b-813d-7284b0c30a70" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_DebtInstrumentCovenantTermsMaximumTotalLeverageRatio_71fc2bb2-f592-4ed7-b062-cdfa89fece16" xlink:href="ango-20210831.xsd#ango_DebtInstrumentCovenantTermsMaximumTotalLeverageRatio"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b0a3ec03-b3c0-42c3-8de4-4aa0a7146dd8" xlink:to="loc_ango_DebtInstrumentCovenantTermsMaximumTotalLeverageRatio_71fc2bb2-f592-4ed7-b062-cdfa89fece16" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_DebtInstrumentCovenantTermsMaximumTotalLeverageRatioSubsequentToMaterialAcquisitions_581f6402-b810-4c38-90ea-714cef4743da" xlink:href="ango-20210831.xsd#ango_DebtInstrumentCovenantTermsMaximumTotalLeverageRatioSubsequentToMaterialAcquisitions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b0a3ec03-b3c0-42c3-8de4-4aa0a7146dd8" xlink:to="loc_ango_DebtInstrumentCovenantTermsMaximumTotalLeverageRatioSubsequentToMaterialAcquisitions_581f6402-b810-4c38-90ea-714cef4743da" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_DebtInstrumentCovenantTermsMinimumFixedChargeCoverageRatio_922d8b33-80bc-4106-85e0-8e152da7834d" xlink:href="ango-20210831.xsd#ango_DebtInstrumentCovenantTermsMinimumFixedChargeCoverageRatio"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b0a3ec03-b3c0-42c3-8de4-4aa0a7146dd8" xlink:to="loc_ango_DebtInstrumentCovenantTermsMinimumFixedChargeCoverageRatio_922d8b33-80bc-4106-85e0-8e152da7834d" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCredit_5010483d-d961-4543-87a2-6d20aa7437ed" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCredit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b0a3ec03-b3c0-42c3-8de4-4aa0a7146dd8" xlink:to="loc_us-gaap_LineOfCredit_5010483d-d961-4543-87a2-6d20aa7437ed" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityInterestRateAtPeriodEnd_25626392-1346-4466-894c-145c42041d89" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityInterestRateAtPeriodEnd"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b0a3ec03-b3c0-42c3-8de4-4aa0a7146dd8" xlink:to="loc_us-gaap_LineOfCreditFacilityInterestRateAtPeriodEnd_25626392-1346-4466-894c-145c42041d89" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_54d2810a-5789-4aee-9c8c-e87c905d366f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b0a3ec03-b3c0-42c3-8de4-4aa0a7146dd8" xlink:to="loc_us-gaap_DebtInstrumentTable_54d2810a-5789-4aee-9c8c-e87c905d366f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_6f44c511-13be-4d59-a7de-93d31392a3a5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_54d2810a-5789-4aee-9c8c-e87c905d366f" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_6f44c511-13be-4d59-a7de-93d31392a3a5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_6f44c511-13be-4d59-a7de-93d31392a3a5_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_6f44c511-13be-4d59-a7de-93d31392a3a5" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_6f44c511-13be-4d59-a7de-93d31392a3a5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_29e4d53f-4d47-494f-8ecf-e892a37ab8c0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_6f44c511-13be-4d59-a7de-93d31392a3a5" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_29e4d53f-4d47-494f-8ecf-e892a37ab8c0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_f1a9de38-e505-4458-a351-b14ff5f89aa9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_29e4d53f-4d47-494f-8ecf-e892a37ab8c0" xlink:to="loc_us-gaap_LineOfCreditMember_f1a9de38-e505-4458-a351-b14ff5f89aa9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_9eb49271-388e-48c9-9a3e-0e444482a5f5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_54d2810a-5789-4aee-9c8c-e87c905d366f" xlink:to="loc_us-gaap_CreditFacilityAxis_9eb49271-388e-48c9-9a3e-0e444482a5f5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_9eb49271-388e-48c9-9a3e-0e444482a5f5_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_9eb49271-388e-48c9-9a3e-0e444482a5f5" xlink:to="loc_us-gaap_CreditFacilityDomain_9eb49271-388e-48c9-9a3e-0e444482a5f5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_3aef3ac1-f4eb-4ee6-abd2-6cea1512934f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_9eb49271-388e-48c9-9a3e-0e444482a5f5" xlink:to="loc_us-gaap_CreditFacilityDomain_3aef3ac1-f4eb-4ee6-abd2-6cea1512934f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevolvingCreditFacilityMember_ae4fe65f-b410-4154-88ad-2164e3e643d6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_3aef3ac1-f4eb-4ee6-abd2-6cea1512934f" xlink:to="loc_us-gaap_RevolvingCreditFacilityMember_ae4fe65f-b410-4154-88ad-2164e3e643d6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_89fa1469-869d-4804-a5f7-e7cbba10d808" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_54d2810a-5789-4aee-9c8c-e87c905d366f" xlink:to="loc_srt_RangeAxis_89fa1469-869d-4804-a5f7-e7cbba10d808" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_89fa1469-869d-4804-a5f7-e7cbba10d808_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_89fa1469-869d-4804-a5f7-e7cbba10d808" xlink:to="loc_srt_RangeMember_89fa1469-869d-4804-a5f7-e7cbba10d808_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_d4f5d0d7-e7bf-4b66-80db-2c36faae7959" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_89fa1469-869d-4804-a5f7-e7cbba10d808" xlink:to="loc_srt_RangeMember_d4f5d0d7-e7bf-4b66-80db-2c36faae7959" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_99407ca1-da94-44a7-b00b-4b45fbfb870a" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_d4f5d0d7-e7bf-4b66-80db-2c36faae7959" xlink:to="loc_srt_MinimumMember_99407ca1-da94-44a7-b00b-4b45fbfb870a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_0b986a7a-454c-494a-8530-6d925f7e3b54" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_d4f5d0d7-e7bf-4b66-80db-2c36faae7959" xlink:to="loc_srt_MaximumMember_0b986a7a-454c-494a-8530-6d925f7e3b54" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateAxis_67fa19f0-c074-464d-a98f-e12f30ef9207" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VariableRateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_54d2810a-5789-4aee-9c8c-e87c905d366f" xlink:to="loc_us-gaap_VariableRateAxis_67fa19f0-c074-464d-a98f-e12f30ef9207" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_67fa19f0-c074-464d-a98f-e12f30ef9207_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VariableRateAxis_67fa19f0-c074-464d-a98f-e12f30ef9207" xlink:to="loc_us-gaap_VariableRateDomain_67fa19f0-c074-464d-a98f-e12f30ef9207_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_ba6f0299-e9f5-4dd6-9b40-014ee5ae99cb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VariableRateAxis_67fa19f0-c074-464d-a98f-e12f30ef9207" xlink:to="loc_us-gaap_VariableRateDomain_ba6f0299-e9f5-4dd6-9b40-014ee5ae99cb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BaseRateMember_26c0f14c-d25f-4e82-8bd0-d37de6494783" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BaseRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_ba6f0299-e9f5-4dd6-9b40-014ee5ae99cb" xlink:to="loc_us-gaap_BaseRateMember_26c0f14c-d25f-4e82-8bd0-d37de6494783" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LondonInterbankOfferedRateLIBORMember_f0e0f4c0-6d39-48f5-90f1-6c9faa770b2d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LondonInterbankOfferedRateLIBORMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_ba6f0299-e9f5-4dd6-9b40-014ee5ae99cb" xlink:to="loc_us-gaap_LondonInterbankOfferedRateLIBORMember_f0e0f4c0-6d39-48f5-90f1-6c9faa770b2d" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/ShareBasedCompensationDetails" xlink:type="simple" xlink:href="ango-20210831.xsd#ShareBasedCompensationDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/ShareBasedCompensationDetails" xlink:type="extended" id="i3c2d51b8897f4d6c85b9acdb27995d44_ShareBasedCompensationDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_5efb98d6-30c9-42d3-9c89-dd53d0175a1e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized_b62720c3-07cf-4315-8fe4-6a65007259df" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_5efb98d6-30c9-42d3-9c89-dd53d0175a1e" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized_b62720c3-07cf-4315-8fe4-6a65007259df" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_d824c42b-0132-4f02-a81d-c7c0fa798e04" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_5efb98d6-30c9-42d3-9c89-dd53d0175a1e" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_d824c42b-0132-4f02-a81d-c7c0fa798e04" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_c9a26f15-812f-47e9-9e7f-f34f6efa7268" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_5efb98d6-30c9-42d3-9c89-dd53d0175a1e" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_c9a26f15-812f-47e9-9e7f-f34f6efa7268" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_6373d3a3-17c0-4095-b46d-65ed23fdfa55" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_5efb98d6-30c9-42d3-9c89-dd53d0175a1e" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_6373d3a3-17c0-4095-b46d-65ed23fdfa55" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_a48c0378-bd22-49b5-af29-a4892dc8f0eb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_5efb98d6-30c9-42d3-9c89-dd53d0175a1e" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_a48c0378-bd22-49b5-af29-a4892dc8f0eb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_f22692c5-8953-40ed-9171-dd090e459100" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_a48c0378-bd22-49b5-af29-a4892dc8f0eb" xlink:to="loc_us-gaap_PlanNameAxis_f22692c5-8953-40ed-9171-dd090e459100" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_f22692c5-8953-40ed-9171-dd090e459100_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_f22692c5-8953-40ed-9171-dd090e459100" xlink:to="loc_us-gaap_PlanNameDomain_f22692c5-8953-40ed-9171-dd090e459100_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_5946bbed-aabf-4973-8408-5f0c6d841fc3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_f22692c5-8953-40ed-9171-dd090e459100" xlink:to="loc_us-gaap_PlanNameDomain_5946bbed-aabf-4973-8408-5f0c6d841fc3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_A2020StockAndIncentiveAwardPlanMember_2ec8ed9b-6197-4be4-b9b2-5af6616ed07e" xlink:href="ango-20210831.xsd#ango_A2020StockAndIncentiveAwardPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_5946bbed-aabf-4973-8408-5f0c6d841fc3" xlink:to="loc_ango_A2020StockAndIncentiveAwardPlanMember_2ec8ed9b-6197-4be4-b9b2-5af6616ed07e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_EmployeeStockPurchasePlanMember_3768710f-f8ee-4df1-8ad0-fd154a509e6a" xlink:href="ango-20210831.xsd#ango_EmployeeStockPurchasePlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_5946bbed-aabf-4973-8408-5f0c6d841fc3" xlink:to="loc_ango_EmployeeStockPurchasePlanMember_3768710f-f8ee-4df1-8ad0-fd154a509e6a" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/SegmentandGeographicInformationSummaryofNetSalesbyProductCategoryDetails" xlink:type="simple" xlink:href="ango-20210831.xsd#SegmentandGeographicInformationSummaryofNetSalesbyProductCategoryDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/SegmentandGeographicInformationSummaryofNetSalesbyProductCategoryDetails" xlink:type="extended" id="i1d2f73aeea0e406d910ee6498c50a7b2_SegmentandGeographicInformationSummaryofNetSalesbyProductCategoryDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_c439c655-f1d6-46c9-8f73-7d4923bdfaef" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_6cf13376-b99a-4946-a92f-d850a50c9429" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_c439c655-f1d6-46c9-8f73-7d4923bdfaef" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_6cf13376-b99a-4946-a92f-d850a50c9429" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_8e8c4c68-515c-4732-ab3f-4b2d34c90b72" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_c439c655-f1d6-46c9-8f73-7d4923bdfaef" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_8e8c4c68-515c-4732-ab3f-4b2d34c90b72" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_0c5bd469-4a82-4962-a49b-803aad630429" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_8e8c4c68-515c-4732-ab3f-4b2d34c90b72" xlink:to="loc_srt_ProductOrServiceAxis_0c5bd469-4a82-4962-a49b-803aad630429" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_0c5bd469-4a82-4962-a49b-803aad630429_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_0c5bd469-4a82-4962-a49b-803aad630429" xlink:to="loc_srt_ProductsAndServicesDomain_0c5bd469-4a82-4962-a49b-803aad630429_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_d854fd1c-a23f-41ea-bfab-37f16ca15fec" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_0c5bd469-4a82-4962-a49b-803aad630429" xlink:to="loc_srt_ProductsAndServicesDomain_d854fd1c-a23f-41ea-bfab-37f16ca15fec" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_EndovascularTherapiesMember_3e67eb63-b637-420b-8e57-721c1d683482" xlink:href="ango-20210831.xsd#ango_EndovascularTherapiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_d854fd1c-a23f-41ea-bfab-37f16ca15fec" xlink:to="loc_ango_EndovascularTherapiesMember_3e67eb63-b637-420b-8e57-721c1d683482" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_VascularAccessMember_3224ea2c-8bb9-4440-9b1f-77aa6c4ec05b" xlink:href="ango-20210831.xsd#ango_VascularAccessMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_d854fd1c-a23f-41ea-bfab-37f16ca15fec" xlink:to="loc_ango_VascularAccessMember_3224ea2c-8bb9-4440-9b1f-77aa6c4ec05b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_OncologyMember_febe86a3-2a4c-4169-a4dc-3a1ec451572f" xlink:href="ango-20210831.xsd#ango_OncologyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_d854fd1c-a23f-41ea-bfab-37f16ca15fec" xlink:to="loc_ango_OncologyMember_febe86a3-2a4c-4169-a4dc-3a1ec451572f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_MedTechMember_14bd1f49-8fc1-416f-8551-1c4dd1d8cc93" xlink:href="ango-20210831.xsd#ango_MedTechMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_d854fd1c-a23f-41ea-bfab-37f16ca15fec" xlink:to="loc_ango_MedTechMember_14bd1f49-8fc1-416f-8551-1c4dd1d8cc93" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_MedDeviceMember_3f3c74bd-3ad9-42ca-95f8-c6a0a7afcbd3" xlink:href="ango-20210831.xsd#ango_MedDeviceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_d854fd1c-a23f-41ea-bfab-37f16ca15fec" xlink:to="loc_ango_MedDeviceMember_3f3c74bd-3ad9-42ca-95f8-c6a0a7afcbd3" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/SegmentandGeographicInformationSummaryofNetSalesbyGeographicAreaDetails" xlink:type="simple" xlink:href="ango-20210831.xsd#SegmentandGeographicInformationSummaryofNetSalesbyGeographicAreaDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/SegmentandGeographicInformationSummaryofNetSalesbyGeographicAreaDetails" xlink:type="extended" id="i9e1d651e58d14338aa8e298843a06c9a_SegmentandGeographicInformationSummaryofNetSalesbyGeographicAreaDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_035c90c8-0ce3-43a2-9a01-a96d046d25e2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_7d174e59-4460-4c5d-ab72-c7405b740ce4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_035c90c8-0ce3-43a2-9a01-a96d046d25e2" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_7d174e59-4460-4c5d-ab72-c7405b740ce4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_262834cd-806e-4904-a757-df3aa7bdfcc4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_035c90c8-0ce3-43a2-9a01-a96d046d25e2" xlink:to="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_262834cd-806e-4904-a757-df3aa7bdfcc4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_98010559-c403-46e5-a048-e0bd2892df3f" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_262834cd-806e-4904-a757-df3aa7bdfcc4" xlink:to="loc_srt_StatementGeographicalAxis_98010559-c403-46e5-a048-e0bd2892df3f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_98010559-c403-46e5-a048-e0bd2892df3f_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_98010559-c403-46e5-a048-e0bd2892df3f" xlink:to="loc_srt_SegmentGeographicalDomain_98010559-c403-46e5-a048-e0bd2892df3f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_66d93f58-eff3-4f10-b1d5-929deba84554" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_98010559-c403-46e5-a048-e0bd2892df3f" xlink:to="loc_srt_SegmentGeographicalDomain_66d93f58-eff3-4f10-b1d5-929deba84554" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_f150c0ee-44fb-4142-a8cf-921670af8799" xlink:href="https://xbrl.sec.gov/country/2020/country-2020-01-31.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_66d93f58-eff3-4f10-b1d5-929deba84554" xlink:to="loc_country_US_f150c0ee-44fb-4142-a8cf-921670af8799" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonUsMember_0bf305d0-3341-4f89-a119-1538bf05ceae" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NonUsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_66d93f58-eff3-4f10-b1d5-929deba84554" xlink:to="loc_us-gaap_NonUsMember_0bf305d0-3341-4f89-a119-1538bf05ceae" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/FairValueFairValueofAssetsandLiabilitiesMeasuredonaRecurringBasisDetails" xlink:type="simple" xlink:href="ango-20210831.xsd#FairValueFairValueofAssetsandLiabilitiesMeasuredonaRecurringBasisDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/FairValueFairValueofAssetsandLiabilitiesMeasuredonaRecurringBasisDetails" xlink:type="extended" id="ie83016e61a194f61a3020ab5cd6688e3_FairValueFairValueofAssetsandLiabilitiesMeasuredonaRecurringBasisDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_50a0ef63-6be5-4832-abc3-a6e119586ffd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_dc752514-30bb-416f-bc2b-61acd9be561c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LiabilitiesFairValueDisclosureAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_50a0ef63-6be5-4832-abc3-a6e119586ffd" xlink:to="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_dc752514-30bb-416f-bc2b-61acd9be561c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_2cfeadc0-7aaa-4bc8-92b3-a25a0fcfbafb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationContingentConsiderationLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_dc752514-30bb-416f-bc2b-61acd9be561c" xlink:to="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_2cfeadc0-7aaa-4bc8-92b3-a25a0fcfbafb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesFairValueDisclosure_04b0ae20-d0e8-4d9e-8fb1-d205e1dd099e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LiabilitiesFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_dc752514-30bb-416f-bc2b-61acd9be561c" xlink:to="loc_us-gaap_LiabilitiesFairValueDisclosure_04b0ae20-d0e8-4d9e-8fb1-d205e1dd099e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_930c8e1c-6bbd-4a53-9577-9377550131d7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_50a0ef63-6be5-4832-abc3-a6e119586ffd" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_930c8e1c-6bbd-4a53-9577-9377550131d7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_7b972fd4-af8d-495c-b6d2-9c2a4b7e68a6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_930c8e1c-6bbd-4a53-9577-9377550131d7" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_7b972fd4-af8d-495c-b6d2-9c2a4b7e68a6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_7b972fd4-af8d-495c-b6d2-9c2a4b7e68a6_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_7b972fd4-af8d-495c-b6d2-9c2a4b7e68a6" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_7b972fd4-af8d-495c-b6d2-9c2a4b7e68a6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_9b5f7412-72de-4cd1-8038-853e336ee744" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_7b972fd4-af8d-495c-b6d2-9c2a4b7e68a6" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_9b5f7412-72de-4cd1-8038-853e336ee744" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_9129348a-f9f1-4808-9f1f-b46aa1668d61" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_9b5f7412-72de-4cd1-8038-853e336ee744" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_9129348a-f9f1-4808-9f1f-b46aa1668d61" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_f019acb5-b132-40f0-83b3-63a46ce975bb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_9b5f7412-72de-4cd1-8038-853e336ee744" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_f019acb5-b132-40f0-83b3-63a46ce975bb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_d1d6cd82-e4fe-4328-90a4-a50248113d9f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_9b5f7412-72de-4cd1-8038-853e336ee744" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_d1d6cd82-e4fe-4328-90a4-a50248113d9f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_7a471440-f43c-4216-84bc-5270eb317977" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_930c8e1c-6bbd-4a53-9577-9377550131d7" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_7a471440-f43c-4216-84bc-5270eb317977" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_7a471440-f43c-4216-84bc-5270eb317977_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_7a471440-f43c-4216-84bc-5270eb317977" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_7a471440-f43c-4216-84bc-5270eb317977_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_95e36c59-c92f-4f2c-8e9c-e2e635704287" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_7a471440-f43c-4216-84bc-5270eb317977" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_95e36c59-c92f-4f2c-8e9c-e2e635704287" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsRecurringMember_fa7fd2b4-9545-4260-b7af-8e5b8eedca53" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsRecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_95e36c59-c92f-4f2c-8e9c-e2e635704287" xlink:to="loc_us-gaap_FairValueMeasurementsRecurringMember_fa7fd2b4-9545-4260-b7af-8e5b8eedca53" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/FairValueSummaryShowingtheRecurringFairValueMeasurementsoftheContingentConsiderationLiabilityDetails" xlink:type="simple" xlink:href="ango-20210831.xsd#FairValueSummaryShowingtheRecurringFairValueMeasurementsoftheContingentConsiderationLiabilityDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/FairValueSummaryShowingtheRecurringFairValueMeasurementsoftheContingentConsiderationLiabilityDetails" xlink:type="extended" id="i7d6ca935e20249e88fe2c9d8a8d34195_FairValueSummaryShowingtheRecurringFairValueMeasurementsoftheContingentConsiderationLiabilityDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_6e23ac34-60bf-403d-a266-7a315830a30d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_62a34253-70c4-4075-9fa7-f155cf94b9ea" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationContingentConsiderationLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_6e23ac34-60bf-403d-a266-7a315830a30d" xlink:to="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_62a34253-70c4-4075-9fa7-f155cf94b9ea" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationContingentConsiderationLiabilityMeasurementInput_0b298a1a-0c30-4872-8b31-1be53c0da252" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationContingentConsiderationLiabilityMeasurementInput"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_6e23ac34-60bf-403d-a266-7a315830a30d" xlink:to="loc_us-gaap_BusinessCombinationContingentConsiderationLiabilityMeasurementInput_0b298a1a-0c30-4872-8b31-1be53c0da252" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_d8d68e8a-8e70-4a0e-90b7-56f19333c44c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_6e23ac34-60bf-403d-a266-7a315830a30d" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_d8d68e8a-8e70-4a0e-90b7-56f19333c44c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeAxis_e389d712-bff2-410a-b33c-e9417a324c42" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MeasurementInputTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_d8d68e8a-8e70-4a0e-90b7-56f19333c44c" xlink:to="loc_us-gaap_MeasurementInputTypeAxis_e389d712-bff2-410a-b33c-e9417a324c42" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeDomain_e389d712-bff2-410a-b33c-e9417a324c42_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MeasurementInputTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_MeasurementInputTypeAxis_e389d712-bff2-410a-b33c-e9417a324c42" xlink:to="loc_us-gaap_MeasurementInputTypeDomain_e389d712-bff2-410a-b33c-e9417a324c42_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeDomain_efe4ce10-9b78-4049-91a9-8b6afb0c4c88" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MeasurementInputTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_MeasurementInputTypeAxis_e389d712-bff2-410a-b33c-e9417a324c42" xlink:to="loc_us-gaap_MeasurementInputTypeDomain_efe4ce10-9b78-4049-91a9-8b6afb0c4c88" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputDiscountRateMember_9c81650a-99df-4b9e-aa89-4ab4ca97a8a5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MeasurementInputDiscountRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MeasurementInputTypeDomain_efe4ce10-9b78-4049-91a9-8b6afb0c4c88" xlink:to="loc_us-gaap_MeasurementInputDiscountRateMember_9c81650a-99df-4b9e-aa89-4ab4ca97a8a5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_MeasurementInputProbabilityofPaymentMember_a19bc1bb-1671-4afd-bd55-15a7b968e8c2" xlink:href="ango-20210831.xsd#ango_MeasurementInputProbabilityofPaymentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MeasurementInputTypeDomain_efe4ce10-9b78-4049-91a9-8b6afb0c4c88" xlink:to="loc_ango_MeasurementInputProbabilityofPaymentMember_a19bc1bb-1671-4afd-bd55-15a7b968e8c2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContingentConsiderationByTypeAxis_5d2f66e9-0b76-4fc8-8e93-b4bb206707f1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContingentConsiderationByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_d8d68e8a-8e70-4a0e-90b7-56f19333c44c" xlink:to="loc_us-gaap_ContingentConsiderationByTypeAxis_5d2f66e9-0b76-4fc8-8e93-b4bb206707f1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContingentConsiderationTypeDomain_5d2f66e9-0b76-4fc8-8e93-b4bb206707f1_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContingentConsiderationTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ContingentConsiderationByTypeAxis_5d2f66e9-0b76-4fc8-8e93-b4bb206707f1" xlink:to="loc_us-gaap_ContingentConsiderationTypeDomain_5d2f66e9-0b76-4fc8-8e93-b4bb206707f1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContingentConsiderationTypeDomain_2d4c84a7-b3ca-4e29-8fa8-75074c866bb2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContingentConsiderationTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ContingentConsiderationByTypeAxis_5d2f66e9-0b76-4fc8-8e93-b4bb206707f1" xlink:to="loc_us-gaap_ContingentConsiderationTypeDomain_2d4c84a7-b3ca-4e29-8fa8-75074c866bb2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_RevenueBasedPaymentMember_d06319d4-e3dc-42a3-ae91-550dffd9224e" xlink:href="ango-20210831.xsd#ango_RevenueBasedPaymentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContingentConsiderationTypeDomain_2d4c84a7-b3ca-4e29-8fa8-75074c866bb2" xlink:to="loc_ango_RevenueBasedPaymentMember_d06319d4-e3dc-42a3-ae91-550dffd9224e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_52d382d7-9653-4f7d-9bfb-777d1395fe2f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_d8d68e8a-8e70-4a0e-90b7-56f19333c44c" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_52d382d7-9653-4f7d-9bfb-777d1395fe2f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_52d382d7-9653-4f7d-9bfb-777d1395fe2f_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_52d382d7-9653-4f7d-9bfb-777d1395fe2f" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_52d382d7-9653-4f7d-9bfb-777d1395fe2f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_b8e6f374-8c4d-4025-a7e0-f39b1547eff1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_52d382d7-9653-4f7d-9bfb-777d1395fe2f" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_b8e6f374-8c4d-4025-a7e0-f39b1547eff1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_1782e49f-b426-41f0-9007-0429ead175c7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_b8e6f374-8c4d-4025-a7e0-f39b1547eff1" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_1782e49f-b426-41f0-9007-0429ead175c7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_32c47600-3875-49dc-af9c-31a5d2902a5e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_d8d68e8a-8e70-4a0e-90b7-56f19333c44c" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_32c47600-3875-49dc-af9c-31a5d2902a5e" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_32c47600-3875-49dc-af9c-31a5d2902a5e_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_32c47600-3875-49dc-af9c-31a5d2902a5e" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_32c47600-3875-49dc-af9c-31a5d2902a5e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_f9a23f33-86ae-4de9-a436-5452629056cc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_32c47600-3875-49dc-af9c-31a5d2902a5e" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_f9a23f33-86ae-4de9-a436-5452629056cc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsRecurringMember_7f09aa69-e0d1-438c-9639-5f5ceb878857" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsRecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_f9a23f33-86ae-4de9-a436-5452629056cc" xlink:to="loc_us-gaap_FairValueMeasurementsRecurringMember_7f09aa69-e0d1-438c-9639-5f5ceb878857" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationTechniqueAxis_23af2bd4-5487-45c7-afe1-c4dd913917f5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ValuationTechniqueAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_d8d68e8a-8e70-4a0e-90b7-56f19333c44c" xlink:to="loc_us-gaap_ValuationTechniqueAxis_23af2bd4-5487-45c7-afe1-c4dd913917f5" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationTechniqueDomain_23af2bd4-5487-45c7-afe1-c4dd913917f5_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ValuationTechniqueDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ValuationTechniqueAxis_23af2bd4-5487-45c7-afe1-c4dd913917f5" xlink:to="loc_us-gaap_ValuationTechniqueDomain_23af2bd4-5487-45c7-afe1-c4dd913917f5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationTechniqueDomain_bffe57be-cf72-429f-8209-26f6c0906eaf" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ValuationTechniqueDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ValuationTechniqueAxis_23af2bd4-5487-45c7-afe1-c4dd913917f5" xlink:to="loc_us-gaap_ValuationTechniqueDomain_bffe57be-cf72-429f-8209-26f6c0906eaf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationTechniqueDiscountedCashFlowMember_3e7b3e1c-1fa9-47cc-8263-7c9f22b7e28c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ValuationTechniqueDiscountedCashFlowMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ValuationTechniqueDomain_bffe57be-cf72-429f-8209-26f6c0906eaf" xlink:to="loc_us-gaap_ValuationTechniqueDiscountedCashFlowMember_3e7b3e1c-1fa9-47cc-8263-7c9f22b7e28c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_8b7d4add-9e64-4179-a79e-8025cd4b1cde" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_d8d68e8a-8e70-4a0e-90b7-56f19333c44c" xlink:to="loc_srt_RangeAxis_8b7d4add-9e64-4179-a79e-8025cd4b1cde" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_8b7d4add-9e64-4179-a79e-8025cd4b1cde_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_8b7d4add-9e64-4179-a79e-8025cd4b1cde" xlink:to="loc_srt_RangeMember_8b7d4add-9e64-4179-a79e-8025cd4b1cde_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_4dba59e4-5439-468a-9a81-81abf16b73a9" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_8b7d4add-9e64-4179-a79e-8025cd4b1cde" xlink:to="loc_srt_RangeMember_4dba59e4-5439-468a-9a81-81abf16b73a9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_e193152b-bf3f-4b6d-9902-b739b7b01706" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_4dba59e4-5439-468a-9a81-81abf16b73a9" xlink:to="loc_srt_MinimumMember_e193152b-bf3f-4b6d-9902-b739b7b01706" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_8d13ba39-392b-45f9-a78e-0d9bc68f717b" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_4dba59e4-5439-468a-9a81-81abf16b73a9" xlink:to="loc_srt_MaximumMember_8d13ba39-392b-45f9-a78e-0d9bc68f717b" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/LeasesSupplementalStatementofOperationsInformationDetails" xlink:type="simple" xlink:href="ango-20210831.xsd#LeasesSupplementalStatementofOperationsInformationDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/LeasesSupplementalStatementofOperationsInformationDetails" xlink:type="extended" id="iecfdf7643a304087b7e7f5697cc9852c_LeasesSupplementalStatementofOperationsInformationDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeLeaseDescriptionLineItems_3a0da6a2-1b79-447a-b656-19497f095aab" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LesseeLeaseDescriptionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseExpense_6bb4f0a6-b90c-42e7-9a65-1612aa9e77a5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingLeaseExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_3a0da6a2-1b79-447a-b656-19497f095aab" xlink:to="loc_us-gaap_OperatingLeaseExpense_6bb4f0a6-b90c-42e7-9a65-1612aa9e77a5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeLeaseDescriptionTable_ccf11a5d-6082-41ac-bd9f-9e3af3fb8c55" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LesseeLeaseDescriptionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_3a0da6a2-1b79-447a-b656-19497f095aab" xlink:to="loc_us-gaap_LesseeLeaseDescriptionTable_ccf11a5d-6082-41ac-bd9f-9e3af3fb8c55" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_4ef96bcc-22cf-420d-956c-d0eba81f1251" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LesseeLeaseDescriptionTable_ccf11a5d-6082-41ac-bd9f-9e3af3fb8c55" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_4ef96bcc-22cf-420d-956c-d0eba81f1251" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_4ef96bcc-22cf-420d-956c-d0eba81f1251_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_4ef96bcc-22cf-420d-956c-d0eba81f1251" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_4ef96bcc-22cf-420d-956c-d0eba81f1251_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_b056ba09-0f57-46f5-848f-8bf855b3707a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_4ef96bcc-22cf-420d-956c-d0eba81f1251" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_b056ba09-0f57-46f5-848f-8bf855b3707a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfSalesMember_aaa4c0df-3e41-42a3-917b-eee5c07da095" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CostOfSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_b056ba09-0f57-46f5-848f-8bf855b3707a" xlink:to="loc_us-gaap_CostOfSalesMember_aaa4c0df-3e41-42a3-917b-eee5c07da095" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpenseMember_49ac8b89-d70d-47c7-977e-9d16111ad249" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ResearchAndDevelopmentExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_b056ba09-0f57-46f5-848f-8bf855b3707a" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpenseMember_49ac8b89-d70d-47c7-977e-9d16111ad249" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingAndMarketingExpenseMember_5278c32f-149e-4b1e-b48d-c888f42056af" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SellingAndMarketingExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_b056ba09-0f57-46f5-848f-8bf855b3707a" xlink:to="loc_us-gaap_SellingAndMarketingExpenseMember_5278c32f-149e-4b1e-b48d-c888f42056af" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpenseMember_06f3cf69-ea04-4028-834d-0099b80a1260" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GeneralAndAdministrativeExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_b056ba09-0f57-46f5-848f-8bf855b3707a" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpenseMember_06f3cf69-ea04-4028-834d-0099b80a1260" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/CommitmentsandContingenciesDetails" xlink:type="simple" xlink:href="ango-20210831.xsd#CommitmentsandContingenciesDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/CommitmentsandContingenciesDetails" xlink:type="extended" id="i3af6ecdfaeb64f6297c0ef8c7ea78c38_CommitmentsandContingenciesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingenciesLineItems_ec2af772-b1ee-4216-bed5-6f995850c2da" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LossContingenciesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_NumberOfPatentsAssertedForReexamination_87165085-8b47-4389-ad0a-820edca37e30" xlink:href="ango-20210831.xsd#ango_NumberOfPatentsAssertedForReexamination"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_ec2af772-b1ee-4216-bed5-6f995850c2da" xlink:to="loc_ango_NumberOfPatentsAssertedForReexamination_87165085-8b47-4389-ad0a-820edca37e30" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_NumberOfPatentsUpheldOverPriorArtReferences_7cd42b4f-a3d3-410e-ae3e-8e2aa8850740" xlink:href="ango-20210831.xsd#ango_NumberOfPatentsUpheldOverPriorArtReferences"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_ec2af772-b1ee-4216-bed5-6f995850c2da" xlink:to="loc_ango_NumberOfPatentsUpheldOverPriorArtReferences_7cd42b4f-a3d3-410e-ae3e-8e2aa8850740" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyPatentsAllegedlyInfringedNumber_1ad4d2cc-9bc6-42af-9c82-3b2ffdd16d65" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LossContingencyPatentsAllegedlyInfringedNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_ec2af772-b1ee-4216-bed5-6f995850c2da" xlink:to="loc_us-gaap_LossContingencyPatentsAllegedlyInfringedNumber_1ad4d2cc-9bc6-42af-9c82-3b2ffdd16d65" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingenciesTable_c81dcbb4-2f98-4f80-9e55-73c5122a199f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LossContingenciesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LossContingenciesLineItems_ec2af772-b1ee-4216-bed5-6f995850c2da" xlink:to="loc_us-gaap_LossContingenciesTable_c81dcbb4-2f98-4f80-9e55-73c5122a199f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_LitigationCaseAxis_ff3191e5-471d-4f26-93f2-b88c3ec03116" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_LitigationCaseAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LossContingenciesTable_c81dcbb4-2f98-4f80-9e55-73c5122a199f" xlink:to="loc_srt_LitigationCaseAxis_ff3191e5-471d-4f26-93f2-b88c3ec03116" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_LitigationCaseTypeDomain_ff3191e5-471d-4f26-93f2-b88c3ec03116_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_LitigationCaseTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_LitigationCaseAxis_ff3191e5-471d-4f26-93f2-b88c3ec03116" xlink:to="loc_srt_LitigationCaseTypeDomain_ff3191e5-471d-4f26-93f2-b88c3ec03116_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_LitigationCaseTypeDomain_390030d5-05dd-4b52-8978-adea564e4634" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_LitigationCaseTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_LitigationCaseAxis_ff3191e5-471d-4f26-93f2-b88c3ec03116" xlink:to="loc_srt_LitigationCaseTypeDomain_390030d5-05dd-4b52-8978-adea564e4634" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_CrBardIncMember_2fb7e37b-52c6-4155-be14-4b7becb45544" xlink:href="ango-20210831.xsd#ango_CrBardIncMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_LitigationCaseTypeDomain_390030d5-05dd-4b52-8978-adea564e4634" xlink:to="loc_ango_CrBardIncMember_2fb7e37b-52c6-4155-be14-4b7becb45544" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_TheDelawareActionMember_529f8379-e915-4794-a2ef-5ef4faef6190" xlink:href="ango-20210831.xsd#ango_TheDelawareActionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_LitigationCaseTypeDomain_390030d5-05dd-4b52-8978-adea564e4634" xlink:to="loc_ango_TheDelawareActionMember_529f8379-e915-4794-a2ef-5ef4faef6190" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AcquisitionRestructuringandOtherItemsNetDetails" xlink:type="simple" xlink:href="ango-20210831.xsd#AcquisitionRestructuringandOtherItemsNetDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AcquisitionRestructuringandOtherItemsNetDetails" xlink:type="extended" id="i2eb31d9665fc4b128cc58e1e81bf3f3c_AcquisitionRestructuringandOtherItemsNetDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringCostAndReserveLineItems_6161749a-3791-4f9e-aac1-24a1bf84b8ee" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestructuringCostAndReserveLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_AcquisitionRestructuringAndOtherItemsNet_fcdd14fb-e85f-4819-8ade-f9c802d1f1b7" xlink:href="ango-20210831.xsd#ango_AcquisitionRestructuringAndOtherItemsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_6161749a-3791-4f9e-aac1-24a1bf84b8ee" xlink:to="loc_ango_AcquisitionRestructuringAndOtherItemsNet_fcdd14fb-e85f-4819-8ade-f9c802d1f1b7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_6226b939-206f-4dd3-9efc-6f5aa5dfd854" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfRestructuringAndRelatedCostsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_6161749a-3791-4f9e-aac1-24a1bf84b8ee" xlink:to="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_6226b939-206f-4dd3-9efc-6f5aa5dfd854" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_ee44c0d1-7cc2-4945-a22e-0d1e30350458" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_6226b939-206f-4dd3-9efc-6f5aa5dfd854" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_ee44c0d1-7cc2-4945-a22e-0d1e30350458" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_ee44c0d1-7cc2-4945-a22e-0d1e30350458_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_ee44c0d1-7cc2-4945-a22e-0d1e30350458" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_ee44c0d1-7cc2-4945-a22e-0d1e30350458_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_a6aef49c-fcba-4364-a881-67c3392313ac" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_ee44c0d1-7cc2-4945-a22e-0d1e30350458" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_a6aef49c-fcba-4364-a881-67c3392313ac" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_LegalCostsMember_0fee4c5c-eafc-45e4-bbe4-2b9f7398ca7d" xlink:href="ango-20210831.xsd#ango_LegalCostsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_a6aef49c-fcba-4364-a881-67c3392313ac" xlink:to="loc_ango_LegalCostsMember_0fee4c5c-eafc-45e4-bbe4-2b9f7398ca7d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_TransitionserviceagreementMember_d4e35e83-ef38-41be-8088-f6cfbfec949a" xlink:href="ango-20210831.xsd#ango_TransitionserviceagreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_a6aef49c-fcba-4364-a881-67c3392313ac" xlink:to="loc_ango_TransitionserviceagreementMember_d4e35e83-ef38-41be-8088-f6cfbfec949a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_DivestitureMember_ee40c5ab-b13e-4660-8e4b-1127d83dc093" xlink:href="ango-20210831.xsd#ango_DivestitureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_a6aef49c-fcba-4364-a881-67c3392313ac" xlink:to="loc_ango_DivestitureMember_ee40c5ab-b13e-4660-8e4b-1127d83dc093" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherExpenseMember_111bd832-ed43-43e5-bd6c-79686a727143" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_a6aef49c-fcba-4364-a881-67c3392313ac" xlink:to="loc_us-gaap_OtherExpenseMember_111bd832-ed43-43e5-bd6c-79686a727143" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AccumulatedOtherComprehensiveIncomeLossDetails" xlink:type="simple" xlink:href="ango-20210831.xsd#AccumulatedOtherComprehensiveIncomeLossDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AccumulatedOtherComprehensiveIncomeLossDetails" xlink:type="extended" id="ib4c2c485ebe34e1ba749ad14d2d1d537_AccumulatedOtherComprehensiveIncomeLossDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_16926ef8-6351-40f9-a0fd-8265309e9279" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_4ba484c9-744d-423e-8e7f-a1f9d5e156cd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AOCIAttributableToParentNetOfTaxRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_16926ef8-6351-40f9-a0fd-8265309e9279" xlink:to="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_4ba484c9-744d-423e-8e7f-a1f9d5e156cd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_0a07fde2-50b2-4554-aac4-0b15bf7dffd7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_4ba484c9-744d-423e-8e7f-a1f9d5e156cd" xlink:to="loc_us-gaap_StockholdersEquity_0a07fde2-50b2-4554-aac4-0b15bf7dffd7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OciBeforeReclassificationsBeforeTaxAttributableToParent_1522473f-38a6-4f18-9fc6-0d7054f9a128" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OciBeforeReclassificationsBeforeTaxAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_4ba484c9-744d-423e-8e7f-a1f9d5e156cd" xlink:to="loc_us-gaap_OciBeforeReclassificationsBeforeTaxAttributableToParent_1522473f-38a6-4f18-9fc6-0d7054f9a128" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_61e3ff5b-c599-4a46-b71d-af9cb05a957a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_4ba484c9-744d-423e-8e7f-a1f9d5e156cd" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_61e3ff5b-c599-4a46-b71d-af9cb05a957a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_4f6abb0a-aeb3-4032-a8b8-b86a56472bd9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_a4507e41-54b7-40e6-937a-e6a1ecc7a2a7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_16926ef8-6351-40f9-a0fd-8265309e9279" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_a4507e41-54b7-40e6-937a-e6a1ecc7a2a7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_6421af7e-5f72-4869-9715-be34f74f40bf" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_a4507e41-54b7-40e6-937a-e6a1ecc7a2a7" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_6421af7e-5f72-4869-9715-be34f74f40bf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_6421af7e-5f72-4869-9715-be34f74f40bf_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_6421af7e-5f72-4869-9715-be34f74f40bf" xlink:to="loc_us-gaap_EquityComponentDomain_6421af7e-5f72-4869-9715-be34f74f40bf_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_80c29835-a3e3-441b-9209-02495e6eedec" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_6421af7e-5f72-4869-9715-be34f74f40bf" xlink:to="loc_us-gaap_EquityComponentDomain_80c29835-a3e3-441b-9209-02495e6eedec" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedTranslationAdjustmentMember_ab302f11-d094-4526-99e5-816b2e1129e5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedTranslationAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_80c29835-a3e3-441b-9209-02495e6eedec" xlink:to="loc_us-gaap_AccumulatedTranslationAdjustmentMember_ab302f11-d094-4526-99e5-816b2e1129e5" xlink:type="arc" order="0"/>
  </link:definitionLink>
</link:linkbase>
