<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with Wdesk from Workiva-->
<!--Copyright 2023 Workiva-->
<!--r:ec04fd8d-b86e-4dd4-b6b0-922a1004078d,g:7ba4c999-43c2-4b42-91d2-0d8d3bf4d1b0-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/CoverPage" xlink:type="simple" xlink:href="ango-20230228.xsd#CoverPage"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/CoverPage" xlink:type="extended" id="i5d77a31bb7034815983e9c0dc1547115_CoverPage">
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_DocumentInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_49e4b935-f54d-460c-a02c-dbe2c9d044e0" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_AmendmentFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_AmendmentFlag_49e4b935-f54d-460c-a02c-dbe2c9d044e0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalYearFocus_fb5de85e-93ee-4b26-94b6-ffbcc336a5fa" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_DocumentFiscalYearFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_DocumentFiscalYearFocus_fb5de85e-93ee-4b26-94b6-ffbcc336a5fa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalPeriodFocus_bff6678c-47c0-4cc0-a88c-13ebcf639396" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_DocumentFiscalPeriodFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_DocumentFiscalPeriodFocus_bff6678c-47c0-4cc0-a88c-13ebcf639396" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_e2c54a17-812e-4ccf-8847-a1f0e89442a8" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityCentralIndexKey"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_EntityCentralIndexKey_e2c54a17-812e-4ccf-8847-a1f0e89442a8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CurrentFiscalYearEndDate_992532ca-c3c5-4ce8-afd2-2cbefedd3ba2" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_CurrentFiscalYearEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_CurrentFiscalYearEndDate_992532ca-c3c5-4ce8-afd2-2cbefedd3ba2" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_NoTradingSymbolFlag_0435937e-2def-4f51-ae51-8cbbd9c4d285" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_NoTradingSymbolFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_NoTradingSymbolFlag_0435937e-2def-4f51-ae51-8cbbd9c4d285" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_8c8e941b-fcb5-45b7-840c-9cf63e2ace0f" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_DocumentType"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_DocumentType_8c8e941b-fcb5-45b7-840c-9cf63e2ace0f" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentQuarterlyReport_c08c6c0c-19aa-4cd0-b3fb-6cec18681423" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_DocumentQuarterlyReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_DocumentQuarterlyReport_c08c6c0c-19aa-4cd0-b3fb-6cec18681423" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentPeriodEndDate_b1f25d27-3c7d-43ad-bca3-a9ea0f8caac5" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_DocumentPeriodEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_DocumentPeriodEndDate_b1f25d27-3c7d-43ad-bca3-a9ea0f8caac5" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentTransitionReport_c6388012-0098-439d-9860-e5d3797e5391" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_DocumentTransitionReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_DocumentTransitionReport_c6388012-0098-439d-9860-e5d3797e5391" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_fb671765-d9a1-49d2-a798-03e3e14094c4" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityFileNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_EntityFileNumber_fb671765-d9a1-49d2-a798-03e3e14094c4" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_181b8438-f1ed-4967-b317-a40f3e77111f" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityRegistrantName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_EntityRegistrantName_181b8438-f1ed-4967-b317-a40f3e77111f" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_b06c1e11-0644-45fe-a9dc-f83d8111f5f5" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_EntityIncorporationStateCountryCode_b06c1e11-0644-45fe-a9dc-f83d8111f5f5" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_23cdbdfe-134f-44b1-8654-a83a168d3238" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_EntityTaxIdentificationNumber_23cdbdfe-134f-44b1-8654-a83a168d3238" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_4ba92440-41ff-4f94-ae2a-3c42129e528f" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityAddressAddressLine1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_EntityAddressAddressLine1_4ba92440-41ff-4f94-ae2a-3c42129e528f" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_bf7acb60-45b4-49e5-b173-d2bf4e7413a2" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityAddressCityOrTown"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_EntityAddressCityOrTown_bf7acb60-45b4-49e5-b173-d2bf4e7413a2" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressStateOrProvince_65b0639f-af28-4868-9d05-8c0680ce2cfe" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityAddressStateOrProvince"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_EntityAddressStateOrProvince_65b0639f-af28-4868-9d05-8c0680ce2cfe" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_7a0af954-c0c4-4fdd-a6f6-ee6f6fa484a4" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityAddressPostalZipCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_EntityAddressPostalZipCode_7a0af954-c0c4-4fdd-a6f6-ee6f6fa484a4" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_a3ed4df0-ed4d-4b0d-bc60-c7bd01359556" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_CityAreaCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_CityAreaCode_a3ed4df0-ed4d-4b0d-bc60-c7bd01359556" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_6158c6f7-cbd3-42e5-a34a-8943c42bf76d" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_LocalPhoneNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_LocalPhoneNumber_6158c6f7-cbd3-42e5-a34a-8943c42bf76d" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_1e38f577-739f-4ff0-b33a-75056ccc03db" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_Security12bTitle"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_Security12bTitle_1e38f577-739f-4ff0-b33a-75056ccc03db" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_7c24f9ca-56c2-4cd6-af47-f726a89322cb" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_TradingSymbol"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_TradingSymbol_7c24f9ca-56c2-4cd6-af47-f726a89322cb" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_14418437-34b5-4f86-a8c3-2203f88113a6" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_SecurityExchangeName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_SecurityExchangeName_14418437-34b5-4f86-a8c3-2203f88113a6" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCurrentReportingStatus_a0384d81-45ee-483a-b33f-a21bf47d4069" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityCurrentReportingStatus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_EntityCurrentReportingStatus_a0384d81-45ee-483a-b33f-a21bf47d4069" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityInteractiveDataCurrent_2167f09f-c3eb-4bae-951c-1b8ab3e25512" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityInteractiveDataCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_EntityInteractiveDataCurrent_2167f09f-c3eb-4bae-951c-1b8ab3e25512" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntitySmallBusiness_1fc2d29c-a3ac-4ab0-9968-d7a70b9a58f5" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntitySmallBusiness"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_EntitySmallBusiness_1fc2d29c-a3ac-4ab0-9968-d7a70b9a58f5" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_095140ce-1b77-4c04-8f31-cb751cd0e9a0" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_EntityEmergingGrowthCompany_095140ce-1b77-4c04-8f31-cb751cd0e9a0" xlink:type="arc" order="26"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityShellCompany_608c78aa-4aad-4cee-aac7-e3d68c94e44a" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityShellCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_EntityShellCompany_608c78aa-4aad-4cee-aac7-e3d68c94e44a" xlink:type="arc" order="27"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCommonStockSharesOutstanding_c5de12f6-3cca-4272-8fa5-88a058e2524f" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityCommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_EntityCommonStockSharesOutstanding_c5de12f6-3cca-4272-8fa5-88a058e2524f" xlink:type="arc" order="28"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFilerCategory_afb20d0d-f2ba-4ff7-b558-98ad16f8aebd" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityFilerCategory"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_EntityFilerCategory_afb20d0d-f2ba-4ff7-b558-98ad16f8aebd" xlink:type="arc" order="29"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentInformationTable_92f26fa1-9b1c-4397-9b75-a38915702275" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_DocumentInformationTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_dei_DocumentInformationLineItems_9d970a35-ad97-464a-b703-f3a7960f03cc" xlink:to="loc_dei_DocumentInformationTable_92f26fa1-9b1c-4397-9b75-a38915702275" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_5912f492-53db-47f7-9444-88e9538c3f97" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_dei_DocumentInformationTable_92f26fa1-9b1c-4397-9b75-a38915702275" xlink:to="loc_us-gaap_StatementClassOfStockAxis_5912f492-53db-47f7-9444-88e9538c3f97" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_5912f492-53db-47f7-9444-88e9538c3f97_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_5912f492-53db-47f7-9444-88e9538c3f97" xlink:to="loc_us-gaap_ClassOfStockDomain_5912f492-53db-47f7-9444-88e9538c3f97_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_3cabb097-5dbc-4bb3-9063-58293eebf9c9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_5912f492-53db-47f7-9444-88e9538c3f97" xlink:to="loc_us-gaap_ClassOfStockDomain_3cabb097-5dbc-4bb3-9063-58293eebf9c9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_0146ea24-c917-4c90-9dc5-7b532d9b2108" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_3cabb097-5dbc-4bb3-9063-58293eebf9c9" xlink:to="loc_us-gaap_CommonStockMember_0146ea24-c917-4c90-9dc5-7b532d9b2108" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_PreferredStockPurchaseRightsMember_6f03ab2d-7a91-44c1-b482-dfd3e02d3fde" xlink:href="ango-20230228.xsd#ango_PreferredStockPurchaseRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_3cabb097-5dbc-4bb3-9063-58293eebf9c9" xlink:to="loc_ango_PreferredStockPurchaseRightsMember_6f03ab2d-7a91-44c1-b482-dfd3e02d3fde" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/ConsolidatedStatementsofStockholdersEquity" xlink:type="simple" xlink:href="ango-20230228.xsd#ConsolidatedStatementsofStockholdersEquity"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/ConsolidatedStatementsofStockholdersEquity" xlink:type="extended" id="i670da8889cbb48e4a26c42fd22e52a46_ConsolidatedStatementsofStockholdersEquity">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_9af4c82d-c31c-4e0c-a06d-d8d0e52794ed" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_d3748b37-03ba-42ce-9112-ab533362631b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncreaseDecreaseInStockholdersEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_9af4c82d-c31c-4e0c-a06d-d8d0e52794ed" xlink:to="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_d3748b37-03ba-42ce-9112-ab533362631b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesOutstanding_2cc3c447-fc8a-4592-a15d-b2c8245ad500" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_d3748b37-03ba-42ce-9112-ab533362631b" xlink:to="loc_us-gaap_SharesOutstanding_2cc3c447-fc8a-4592-a15d-b2c8245ad500" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_95402442-6847-46fb-8e9b-db0fd4ade467" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_d3748b37-03ba-42ce-9112-ab533362631b" xlink:to="loc_us-gaap_StockholdersEquity_95402442-6847-46fb-8e9b-db0fd4ade467" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockCommonShares_98160b5e-288d-41f4-b37d-190ca6e3b6fe" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TreasuryStockCommonShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_d3748b37-03ba-42ce-9112-ab533362631b" xlink:to="loc_us-gaap_TreasuryStockCommonShares_98160b5e-288d-41f4-b37d-190ca6e3b6fe" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_3946a2e2-5742-4ad1-8cb3-e59f25594d04" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_d3748b37-03ba-42ce-9112-ab533362631b" xlink:to="loc_us-gaap_NetIncomeLoss_3946a2e2-5742-4ad1-8cb3-e59f25594d04" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_eed51b42-996e-4bdf-96d3-dd48cd4a9a76" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_d3748b37-03ba-42ce-9112-ab533362631b" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_eed51b42-996e-4bdf-96d3-dd48cd4a9a76" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_81313fb5-3e18-4840-ab8d-1095c96a61a8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_d3748b37-03ba-42ce-9112-ab533362631b" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_81313fb5-3e18-4840-ab8d-1095c96a61a8" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation_5b3a1e48-cd81-49aa-9040-f1b3156c99a7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_d3748b37-03ba-42ce-9112-ab533362631b" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation_5b3a1e48-cd81-49aa-9040-f1b3156c99a7" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation_ccd9cd2e-5f4d-492e-a60d-a472301b719f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_d3748b37-03ba-42ce-9112-ab533362631b" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation_ccd9cd2e-5f4d-492e-a60d-a472301b719f" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans_64b84950-54b3-43c4-9668-8304f93f20ee" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_d3748b37-03ba-42ce-9112-ab533362631b" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans_64b84950-54b3-43c4-9668-8304f93f20ee" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan_0fc3b481-c419-41e5-acdb-5c59bf11819d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_d3748b37-03ba-42ce-9112-ab533362631b" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan_0fc3b481-c419-41e5-acdb-5c59bf11819d" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_9e949f57-e274-45ea-a34f-fe6b02c836a3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_d3748b37-03ba-42ce-9112-ab533362631b" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_9e949f57-e274-45ea-a34f-fe6b02c836a3" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_7ec243ea-ccc9-41a8-82db-f4bd2ea4c2de" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_d3748b37-03ba-42ce-9112-ab533362631b" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_7ec243ea-ccc9-41a8-82db-f4bd2ea4c2de" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesOutstanding_a131e079-aab0-4660-bd6c-fb2c28ffb578" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_4789424d-8ae4-4fb8-9bba-a34cf1b18756" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockCommonShares_1c42dc04-11ca-41df-977f-a99127899149" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TreasuryStockCommonShares"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_d7fd40c7-05aa-4379-bbf4-706586daad33" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_9af4c82d-c31c-4e0c-a06d-d8d0e52794ed" xlink:to="loc_us-gaap_StatementTable_d7fd40c7-05aa-4379-bbf4-706586daad33" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_e28fa9fb-45c2-4ee8-958e-0158e054c82c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_d7fd40c7-05aa-4379-bbf4-706586daad33" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_e28fa9fb-45c2-4ee8-958e-0158e054c82c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_e28fa9fb-45c2-4ee8-958e-0158e054c82c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_e28fa9fb-45c2-4ee8-958e-0158e054c82c" xlink:to="loc_us-gaap_EquityComponentDomain_e28fa9fb-45c2-4ee8-958e-0158e054c82c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_0ddb9fed-1bd8-4314-8b26-2794bde6709d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_e28fa9fb-45c2-4ee8-958e-0158e054c82c" xlink:to="loc_us-gaap_EquityComponentDomain_0ddb9fed-1bd8-4314-8b26-2794bde6709d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_a7115d46-5996-4d41-a2a7-b16ed4272e7d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_0ddb9fed-1bd8-4314-8b26-2794bde6709d" xlink:to="loc_us-gaap_CommonStockMember_a7115d46-5996-4d41-a2a7-b16ed4272e7d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_08f4ecf7-1530-48cd-bf08-373637081188" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_0ddb9fed-1bd8-4314-8b26-2794bde6709d" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_08f4ecf7-1530-48cd-bf08-373637081188" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_f81df035-7f9d-487c-bb7b-0f24a6e0ca17" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_0ddb9fed-1bd8-4314-8b26-2794bde6709d" xlink:to="loc_us-gaap_RetainedEarningsMember_f81df035-7f9d-487c-bb7b-0f24a6e0ca17" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_75564325-e419-49c4-9626-b816c31f45e5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_0ddb9fed-1bd8-4314-8b26-2794bde6709d" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_75564325-e419-49c4-9626-b816c31f45e5" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockCommonMember_1a38afb0-51c6-443f-b7c4-97e7c8955b1c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TreasuryStockCommonMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_0ddb9fed-1bd8-4314-8b26-2794bde6709d" xlink:to="loc_us-gaap_TreasuryStockCommonMember_1a38afb0-51c6-443f-b7c4-97e7c8955b1c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_553aef6b-edd6-4034-aeb9-bfac2ffca791" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_d7fd40c7-05aa-4379-bbf4-706586daad33" xlink:to="loc_us-gaap_AwardTypeAxis_553aef6b-edd6-4034-aeb9-bfac2ffca791" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_553aef6b-edd6-4034-aeb9-bfac2ffca791_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_553aef6b-edd6-4034-aeb9-bfac2ffca791" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_553aef6b-edd6-4034-aeb9-bfac2ffca791_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_3f845641-9b96-4678-b86d-cd40a6dac801" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_553aef6b-edd6-4034-aeb9-bfac2ffca791" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_3f845641-9b96-4678-b86d-cd40a6dac801" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_dc032261-dfe0-4c34-8ed3-da14ac77be47" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_3f845641-9b96-4678-b86d-cd40a6dac801" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_dc032261-dfe0-4c34-8ed3-da14ac77be47" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PerformanceSharesMember_8436c906-325a-47ab-afca-06ca0e56b709" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PerformanceSharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_3f845641-9b96-4678-b86d-cd40a6dac801" xlink:to="loc_us-gaap_PerformanceSharesMember_8436c906-325a-47ab-afca-06ca0e56b709" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AcquisitionsDetails" xlink:type="simple" xlink:href="ango-20230228.xsd#AcquisitionsDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AcquisitionsDetails" xlink:type="extended" id="ibde74f27729f49bb8abc4e89e9863224_AcquisitionsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionLineItems_1ea02c22-e9a7-4a86-99a5-878c57fb2c7c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_BusinessAcquisitionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetAcquisitionConsiderationTransferred_cd7bb33f-c142-4b7a-b6bc-a2d3e9790a8a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AssetAcquisitionConsiderationTransferred"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_1ea02c22-e9a7-4a86-99a5-878c57fb2c7c" xlink:to="loc_us-gaap_AssetAcquisitionConsiderationTransferred_cd7bb33f-c142-4b7a-b6bc-a2d3e9790a8a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiability_d1ec8941-2e77-48a5-b4c8-7dbba41ce518" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ContractWithCustomerLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_1ea02c22-e9a7-4a86-99a5-878c57fb2c7c" xlink:to="loc_us-gaap_ContractWithCustomerLiability_d1ec8941-2e77-48a5-b4c8-7dbba41ce518" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityCurrent_599f17ae-5cde-4c58-9f1b-5ff8cf365d6b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ContractWithCustomerLiabilityCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_1ea02c22-e9a7-4a86-99a5-878c57fb2c7c" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityCurrent_599f17ae-5cde-4c58-9f1b-5ff8cf365d6b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_RevenueRemainingPerformanceObligationVariableConsiderationAmount_d6727112-8ee2-4560-b4d5-66a52dc80b14" xlink:href="ango-20230228.xsd#ango_RevenueRemainingPerformanceObligationVariableConsiderationAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_1ea02c22-e9a7-4a86-99a5-878c57fb2c7c" xlink:to="loc_ango_RevenueRemainingPerformanceObligationVariableConsiderationAmount_d6727112-8ee2-4560-b4d5-66a52dc80b14" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_AssetAcquisitionInventoryAndFixedAssets_61807799-6a87-492d-b38e-756fb37be76d" xlink:href="ango-20230228.xsd#ango_AssetAcquisitionInventoryAndFixedAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_1ea02c22-e9a7-4a86-99a5-878c57fb2c7c" xlink:to="loc_ango_AssetAcquisitionInventoryAndFixedAssets_61807799-6a87-492d-b38e-756fb37be76d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinitelivedIntangibleAssetsAcquired1_556b05b4-8aa6-4a91-918c-6a15b0e0843f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinitelivedIntangibleAssetsAcquired1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_1ea02c22-e9a7-4a86-99a5-878c57fb2c7c" xlink:to="loc_us-gaap_FinitelivedIntangibleAssetsAcquired1_556b05b4-8aa6-4a91-918c-6a15b0e0843f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsRemainingAmortizationPeriod1_2358efeb-7a2f-497b-a246-7697fe9e92a9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsRemainingAmortizationPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_1ea02c22-e9a7-4a86-99a5-878c57fb2c7c" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsRemainingAmortizationPeriod1_2358efeb-7a2f-497b-a246-7697fe9e92a9" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_b3558b70-2f08-4e76-be44-29d54b6c6660" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_1ea02c22-e9a7-4a86-99a5-878c57fb2c7c" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_b3558b70-2f08-4e76-be44-29d54b6c6660" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_9730449c-940c-4cf8-93bd-1561e6ba18b9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_b3558b70-2f08-4e76-be44-29d54b6c6660" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_9730449c-940c-4cf8-93bd-1561e6ba18b9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_9730449c-940c-4cf8-93bd-1561e6ba18b9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_9730449c-940c-4cf8-93bd-1561e6ba18b9" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_9730449c-940c-4cf8-93bd-1561e6ba18b9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_4279b7a1-3126-4c84-85cb-df877183f59f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_9730449c-940c-4cf8-93bd-1561e6ba18b9" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_4279b7a1-3126-4c84-85cb-df877183f59f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TechnologyBasedIntangibleAssetsMember_9c8889ac-54f2-424e-a0ae-ee3e0b19c9d7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TechnologyBasedIntangibleAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_4279b7a1-3126-4c84-85cb-df877183f59f" xlink:to="loc_us-gaap_TechnologyBasedIntangibleAssetsMember_9c8889ac-54f2-424e-a0ae-ee3e0b19c9d7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetAcquisitionAxis_6d78bded-4dd7-4e11-acb7-43acb693f513" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AssetAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_b3558b70-2f08-4e76-be44-29d54b6c6660" xlink:to="loc_us-gaap_AssetAcquisitionAxis_6d78bded-4dd7-4e11-acb7-43acb693f513" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetAcquisitionDomain_6d78bded-4dd7-4e11-acb7-43acb693f513_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AssetAcquisitionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AssetAcquisitionAxis_6d78bded-4dd7-4e11-acb7-43acb693f513" xlink:to="loc_us-gaap_AssetAcquisitionDomain_6d78bded-4dd7-4e11-acb7-43acb693f513_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetAcquisitionDomain_d9b26252-3992-410f-b3fb-3b4d3ed4d769" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AssetAcquisitionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AssetAcquisitionAxis_6d78bded-4dd7-4e11-acb7-43acb693f513" xlink:to="loc_us-gaap_AssetAcquisitionDomain_d9b26252-3992-410f-b3fb-3b4d3ed4d769" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_QXMedicalLLCCamaroSupportCatheterAssetAcquisitionMember_07b197ab-8e88-4228-a422-b7c60e92666a" xlink:href="ango-20230228.xsd#ango_QXMedicalLLCCamaroSupportCatheterAssetAcquisitionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetAcquisitionDomain_d9b26252-3992-410f-b3fb-3b4d3ed4d769" xlink:to="loc_ango_QXMedicalLLCCamaroSupportCatheterAssetAcquisitionMember_07b197ab-8e88-4228-a422-b7c60e92666a" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/RevenuefromContractswithCustomersDisaggregationofRevenueDetails" xlink:type="simple" xlink:href="ango-20230228.xsd#RevenuefromContractswithCustomersDisaggregationofRevenueDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/RevenuefromContractswithCustomersDisaggregationofRevenueDetails" xlink:type="extended" id="i272fcfdbf5da4b6e8c3d90ebc6722089_RevenuefromContractswithCustomersDisaggregationofRevenueDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_9ee9fb0d-5035-4002-ac3f-139950377649" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_ca531292-a6e1-471a-b381-7876c7ea9324" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_9ee9fb0d-5035-4002-ac3f-139950377649" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_ca531292-a6e1-471a-b381-7876c7ea9324" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueTable_df2fa624-b0d3-4650-94ea-482884e310f6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DisaggregationOfRevenueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_9ee9fb0d-5035-4002-ac3f-139950377649" xlink:to="loc_us-gaap_DisaggregationOfRevenueTable_df2fa624-b0d3-4650-94ea-482884e310f6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_b7ed0f7d-ff87-4c49-a81c-479c5b519fa3" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_df2fa624-b0d3-4650-94ea-482884e310f6" xlink:to="loc_srt_ProductOrServiceAxis_b7ed0f7d-ff87-4c49-a81c-479c5b519fa3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_b7ed0f7d-ff87-4c49-a81c-479c5b519fa3_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_b7ed0f7d-ff87-4c49-a81c-479c5b519fa3" xlink:to="loc_srt_ProductsAndServicesDomain_b7ed0f7d-ff87-4c49-a81c-479c5b519fa3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_4ffeabc4-117f-4572-b16a-851c7047ad27" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_b7ed0f7d-ff87-4c49-a81c-479c5b519fa3" xlink:to="loc_srt_ProductsAndServicesDomain_4ffeabc4-117f-4572-b16a-851c7047ad27" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_MedTechMember_68ec1f0d-ebb8-4e90-8d30-7cfa94c92bb4" xlink:href="ango-20230228.xsd#ango_MedTechMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_4ffeabc4-117f-4572-b16a-851c7047ad27" xlink:to="loc_ango_MedTechMember_68ec1f0d-ebb8-4e90-8d30-7cfa94c92bb4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_MedDeviceMember_efd92ca2-e004-4a28-8add-7ca292ab7f15" xlink:href="ango-20230228.xsd#ango_MedDeviceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_4ffeabc4-117f-4572-b16a-851c7047ad27" xlink:to="loc_ango_MedDeviceMember_efd92ca2-e004-4a28-8add-7ca292ab7f15" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_f56d0253-990e-4923-ba23-d25be583dce7" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_df2fa624-b0d3-4650-94ea-482884e310f6" xlink:to="loc_srt_StatementGeographicalAxis_f56d0253-990e-4923-ba23-d25be583dce7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_f56d0253-990e-4923-ba23-d25be583dce7_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_f56d0253-990e-4923-ba23-d25be583dce7" xlink:to="loc_srt_SegmentGeographicalDomain_f56d0253-990e-4923-ba23-d25be583dce7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_8a95f472-e5a4-456b-be66-6c1e620bf7b1" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_f56d0253-990e-4923-ba23-d25be583dce7" xlink:to="loc_srt_SegmentGeographicalDomain_8a95f472-e5a4-456b-be66-6c1e620bf7b1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_0c74c9b1-92e1-4c20-ba81-5ea224b91f24" xlink:href="https://xbrl.sec.gov/country/2021/country-2021.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_8a95f472-e5a4-456b-be66-6c1e620bf7b1" xlink:to="loc_country_US_0c74c9b1-92e1-4c20-ba81-5ea224b91f24" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonUsMember_f93eabeb-47fd-48a3-af69-34410e2dbb48" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NonUsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_8a95f472-e5a4-456b-be66-6c1e620bf7b1" xlink:to="loc_us-gaap_NonUsMember_f93eabeb-47fd-48a3-af69-34410e2dbb48" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/RevenuefromContractswithCustomersNarrativeDetails" xlink:type="simple" xlink:href="ango-20230228.xsd#RevenuefromContractswithCustomersNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/RevenuefromContractswithCustomersNarrativeDetails" xlink:type="extended" id="i7d52bf132b5048e2bd79ce21dcd211f1_RevenuefromContractswithCustomersNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_d5434878-e7eb-44e3-8ae5-4548238b1609" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_RevenuePerformanceObligationPaymentTerm_edf0df0b-b17a-4b13-8fad-96160b8e87fb" xlink:href="ango-20230228.xsd#ango_RevenuePerformanceObligationPaymentTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_d5434878-e7eb-44e3-8ae5-4548238b1609" xlink:to="loc_ango_RevenuePerformanceObligationPaymentTerm_edf0df0b-b17a-4b13-8fad-96160b8e87fb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_ContractWithCustomerRightOfReturnProductReturnPeriodAfterPurchaseDateRequiringPreApproval_cbe1104e-edb7-4a41-af2e-ae326625b7f5" xlink:href="ango-20230228.xsd#ango_ContractWithCustomerRightOfReturnProductReturnPeriodAfterPurchaseDateRequiringPreApproval"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_d5434878-e7eb-44e3-8ae5-4548238b1609" xlink:to="loc_ango_ContractWithCustomerRightOfReturnProductReturnPeriodAfterPurchaseDateRequiringPreApproval_cbe1104e-edb7-4a41-af2e-ae326625b7f5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_ContractWithCustomerRightOfReturnRestockingCharge_52c35479-a851-475f-a464-4df83931b7fa" xlink:href="ango-20230228.xsd#ango_ContractWithCustomerRightOfReturnRestockingCharge"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_d5434878-e7eb-44e3-8ae5-4548238b1609" xlink:to="loc_ango_ContractWithCustomerRightOfReturnRestockingCharge_52c35479-a851-475f-a464-4df83931b7fa" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_ContractWithCustomerRightOfReturnMinimumRemainingProductLifePriorToReturn_cfe6ee59-2eda-482e-bff4-e231be715a9c" xlink:href="ango-20230228.xsd#ango_ContractWithCustomerRightOfReturnMinimumRemainingProductLifePriorToReturn"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_d5434878-e7eb-44e3-8ae5-4548238b1609" xlink:to="loc_ango_ContractWithCustomerRightOfReturnMinimumRemainingProductLifePriorToReturn_cfe6ee59-2eda-482e-bff4-e231be715a9c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityIncreaseDecreaseForContractAcquiredInBusinessCombination_f6e43397-61c1-4615-abcc-9fc53527aa5a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ContractWithCustomerLiabilityIncreaseDecreaseForContractAcquiredInBusinessCombination"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_d5434878-e7eb-44e3-8ae5-4548238b1609" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityIncreaseDecreaseForContractAcquiredInBusinessCombination_f6e43397-61c1-4615-abcc-9fc53527aa5a" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_CustomerWithCustomerLiabilityRevenueRecognizedIncludingCurrentPeriod_95bdda1c-e32f-4faa-a2d5-c5fbf52b81f9" xlink:href="ango-20230228.xsd#ango_CustomerWithCustomerLiabilityRevenueRecognizedIncludingCurrentPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_d5434878-e7eb-44e3-8ae5-4548238b1609" xlink:to="loc_ango_CustomerWithCustomerLiabilityRevenueRecognizedIncludingCurrentPeriod_95bdda1c-e32f-4faa-a2d5-c5fbf52b81f9" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueTable_43a59acc-24ee-4e71-a41b-6143e64a429f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DisaggregationOfRevenueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_d5434878-e7eb-44e3-8ae5-4548238b1609" xlink:to="loc_us-gaap_DisaggregationOfRevenueTable_43a59acc-24ee-4e71-a41b-6143e64a429f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_d68d6a30-4508-4ccf-b8e8-ff2304b065b0" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_43a59acc-24ee-4e71-a41b-6143e64a429f" xlink:to="loc_srt_RangeAxis_d68d6a30-4508-4ccf-b8e8-ff2304b065b0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_d68d6a30-4508-4ccf-b8e8-ff2304b065b0_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_d68d6a30-4508-4ccf-b8e8-ff2304b065b0" xlink:to="loc_srt_RangeMember_d68d6a30-4508-4ccf-b8e8-ff2304b065b0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_d9c0ee8e-4494-414d-9bf9-66d33c2f4009" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_d68d6a30-4508-4ccf-b8e8-ff2304b065b0" xlink:to="loc_srt_RangeMember_d9c0ee8e-4494-414d-9bf9-66d33c2f4009" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_c89281bc-ec98-4654-9304-452ed0f87373" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_d9c0ee8e-4494-414d-9bf9-66d33c2f4009" xlink:to="loc_srt_MinimumMember_c89281bc-ec98-4654-9304-452ed0f87373" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_e7a4b5e5-f466-4b1e-b0ab-99112c73b128" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_d9c0ee8e-4494-414d-9bf9-66d33c2f4009" xlink:to="loc_srt_MaximumMember_e7a4b5e5-f466-4b1e-b0ab-99112c73b128" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/GoodwillandIntangibleAssetsNarrativeDetails" xlink:type="simple" xlink:href="ango-20230228.xsd#GoodwillandIntangibleAssetsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/GoodwillandIntangibleAssetsNarrativeDetails" xlink:type="extended" id="i4a7cb9c507cd43f18cad3813f103960a_GoodwillandIntangibleAssetsNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_0bc6cf40-3cd7-4620-bfc7-6523b781184d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfReportingUnits_843292a4-492d-4946-9c62-bc0cf3900fb6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NumberOfReportingUnits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_0bc6cf40-3cd7-4620-bfc7-6523b781184d" xlink:to="loc_us-gaap_NumberOfReportingUnits_843292a4-492d-4946-9c62-bc0cf3900fb6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfOperatingSegments_f4743f08-aed2-4ae8-b490-83a89910a32f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NumberOfOperatingSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_0bc6cf40-3cd7-4620-bfc7-6523b781184d" xlink:to="loc_us-gaap_NumberOfOperatingSegments_f4743f08-aed2-4ae8-b490-83a89910a32f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillImpairmentLoss_ac4512ef-1999-4a85-a4fc-3ecea8b6b0cd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_GoodwillImpairmentLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_0bc6cf40-3cd7-4620-bfc7-6523b781184d" xlink:to="loc_us-gaap_GoodwillImpairmentLoss_ac4512ef-1999-4a85-a4fc-3ecea8b6b0cd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_b328b71e-10f7-4934-b32d-816d29b442f4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_0bc6cf40-3cd7-4620-bfc7-6523b781184d" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_b328b71e-10f7-4934-b32d-816d29b442f4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfIntangibleAssets_b95757fa-b9db-494d-813e-c43821edd0fa" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AmortizationOfIntangibleAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_0bc6cf40-3cd7-4620-bfc7-6523b781184d" xlink:to="loc_us-gaap_AmortizationOfIntangibleAssets_b95757fa-b9db-494d-813e-c43821edd0fa" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_e61220bd-3104-4665-8f1b-773a1bb2144a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_0bc6cf40-3cd7-4620-bfc7-6523b781184d" xlink:to="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_e61220bd-3104-4665-8f1b-773a1bb2144a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_76fb3d57-8074-4039-9fd4-857b4cb664f5" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_e61220bd-3104-4665-8f1b-773a1bb2144a" xlink:to="loc_srt_RangeAxis_76fb3d57-8074-4039-9fd4-857b4cb664f5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_76fb3d57-8074-4039-9fd4-857b4cb664f5_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_76fb3d57-8074-4039-9fd4-857b4cb664f5" xlink:to="loc_srt_RangeMember_76fb3d57-8074-4039-9fd4-857b4cb664f5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_fdffbe65-4a7e-4c4e-9174-986a4396be80" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_76fb3d57-8074-4039-9fd4-857b4cb664f5" xlink:to="loc_srt_RangeMember_fdffbe65-4a7e-4c4e-9174-986a4396be80" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_9b549f07-f4ad-4136-bfd0-2dbe2af1edc5" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_fdffbe65-4a7e-4c4e-9174-986a4396be80" xlink:to="loc_srt_MinimumMember_9b549f07-f4ad-4136-bfd0-2dbe2af1edc5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_a4b38382-d79c-48be-8d66-61c65cfd09e0" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_fdffbe65-4a7e-4c4e-9174-986a4396be80" xlink:to="loc_srt_MaximumMember_a4b38382-d79c-48be-8d66-61c65cfd09e0" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/GoodwillandIntangibleAssetsChangeinGoodwillDetails" xlink:type="simple" xlink:href="ango-20230228.xsd#GoodwillandIntangibleAssetsChangeinGoodwillDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/GoodwillandIntangibleAssetsChangeinGoodwillDetails" xlink:type="extended" id="i2cc97c3583884b0186c0634407a6ed0f_GoodwillandIntangibleAssetsChangeinGoodwillDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillLineItems_3c6f8678-520c-4d6c-b786-430bc47469a2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_GoodwillLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillRollForward_8850be48-9412-4b17-bc58-01b0dce2b91f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_GoodwillRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GoodwillLineItems_3c6f8678-520c-4d6c-b786-430bc47469a2" xlink:to="loc_us-gaap_GoodwillRollForward_8850be48-9412-4b17-bc58-01b0dce2b91f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_1265c893-2714-4780-9086-16c71bb12134" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GoodwillRollForward_8850be48-9412-4b17-bc58-01b0dce2b91f" xlink:to="loc_us-gaap_Goodwill_1265c893-2714-4780-9086-16c71bb12134" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillForeignCurrencyTranslationGainLoss_344ef3a3-97e2-48d7-9214-912c26e2b855" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_GoodwillForeignCurrencyTranslationGainLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GoodwillRollForward_8850be48-9412-4b17-bc58-01b0dce2b91f" xlink:to="loc_us-gaap_GoodwillForeignCurrencyTranslationGainLoss_344ef3a3-97e2-48d7-9214-912c26e2b855" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_1fe8fb91-5e83-47ca-a3e3-8bda68adfada" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_Goodwill"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfGoodwillTable_7b462e9e-3a06-48d1-829c-f3f2ee950509" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfGoodwillTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_GoodwillLineItems_3c6f8678-520c-4d6c-b786-430bc47469a2" xlink:to="loc_us-gaap_ScheduleOfGoodwillTable_7b462e9e-3a06-48d1-829c-f3f2ee950509" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReportingUnitAxis_e41b6b61-6083-4545-a36b-be3adba7d146" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ReportingUnitAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfGoodwillTable_7b462e9e-3a06-48d1-829c-f3f2ee950509" xlink:to="loc_us-gaap_ReportingUnitAxis_e41b6b61-6083-4545-a36b-be3adba7d146" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReportingUnitDomain_e41b6b61-6083-4545-a36b-be3adba7d146_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ReportingUnitDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ReportingUnitAxis_e41b6b61-6083-4545-a36b-be3adba7d146" xlink:to="loc_us-gaap_ReportingUnitDomain_e41b6b61-6083-4545-a36b-be3adba7d146_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReportingUnitDomain_a6170700-7acd-4384-89f7-88289642958d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ReportingUnitDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ReportingUnitAxis_e41b6b61-6083-4545-a36b-be3adba7d146" xlink:to="loc_us-gaap_ReportingUnitDomain_a6170700-7acd-4384-89f7-88289642958d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_MedTechMember_bc460c31-7aee-4dec-acd8-27d53b70c177" xlink:href="ango-20230228.xsd#ango_MedTechMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReportingUnitDomain_a6170700-7acd-4384-89f7-88289642958d" xlink:to="loc_ango_MedTechMember_bc460c31-7aee-4dec-acd8-27d53b70c177" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_MedDeviceMember_5b9c617c-c82d-4aa3-8eee-56cfe00e87da" xlink:href="ango-20230228.xsd#ango_MedDeviceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReportingUnitDomain_a6170700-7acd-4384-89f7-88289642958d" xlink:to="loc_ango_MedDeviceMember_5b9c617c-c82d-4aa3-8eee-56cfe00e87da" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/GoodwillandIntangibleAssetsIntangibleAssetsDetails" xlink:type="simple" xlink:href="ango-20230228.xsd#GoodwillandIntangibleAssetsIntangibleAssetsDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/GoodwillandIntangibleAssetsIntangibleAssetsDetails" xlink:type="extended" id="icdc57f6a5c4a4e608893cbc933855e8c_GoodwillandIntangibleAssetsIntangibleAssetsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_d5e7cb88-6caa-4771-86ab-519eef8b8a4e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_37383a67-1ec9-4da9-a0ba-b93b2237298f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_d5e7cb88-6caa-4771-86ab-519eef8b8a4e" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_37383a67-1ec9-4da9-a0ba-b93b2237298f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_b6fac31d-d997-4a94-837f-d5d52ca6f3de" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_d5e7cb88-6caa-4771-86ab-519eef8b8a4e" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_b6fac31d-d997-4a94-837f-d5d52ca6f3de" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_9976345d-6f49-4c9b-9e03-d510ece1fc14" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_d5e7cb88-6caa-4771-86ab-519eef8b8a4e" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNet_9976345d-6f49-4c9b-9e03-d510ece1fc14" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_47431e14-1d04-415e-8566-3dfa339231b8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_d5e7cb88-6caa-4771-86ab-519eef8b8a4e" xlink:to="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_47431e14-1d04-415e-8566-3dfa339231b8" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_279ceef9-f7bb-4d61-94e5-eda983a55d3a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_47431e14-1d04-415e-8566-3dfa339231b8" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_279ceef9-f7bb-4d61-94e5-eda983a55d3a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_279ceef9-f7bb-4d61-94e5-eda983a55d3a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_279ceef9-f7bb-4d61-94e5-eda983a55d3a" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_279ceef9-f7bb-4d61-94e5-eda983a55d3a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1cd17938-fbe0-4edf-98f4-2869aa939fa4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_279ceef9-f7bb-4d61-94e5-eda983a55d3a" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1cd17938-fbe0-4edf-98f4-2869aa939fa4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_ProductTechnologiesMember_41a5ca16-7190-49d1-b36b-73e5ac18717b" xlink:href="ango-20230228.xsd#ango_ProductTechnologiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1cd17938-fbe0-4edf-98f4-2869aa939fa4" xlink:to="loc_ango_ProductTechnologiesMember_41a5ca16-7190-49d1-b36b-73e5ac18717b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerRelationshipsMember_8ca10762-fd02-464c-bfe0-5e178c54ede2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CustomerRelationshipsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1cd17938-fbe0-4edf-98f4-2869aa939fa4" xlink:to="loc_us-gaap_CustomerRelationshipsMember_8ca10762-fd02-464c-bfe0-5e178c54ede2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TrademarksMember_f19a83f5-ce86-4817-9993-50b761548881" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TrademarksMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1cd17938-fbe0-4edf-98f4-2869aa939fa4" xlink:to="loc_us-gaap_TrademarksMember_f19a83f5-ce86-4817-9993-50b761548881" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LicenseAgreementTermsMember_0c71e8b9-b13a-4436-9d93-f8aeefd723be" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LicenseAgreementTermsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1cd17938-fbe0-4edf-98f4-2869aa939fa4" xlink:to="loc_us-gaap_LicenseAgreementTermsMember_0c71e8b9-b13a-4436-9d93-f8aeefd723be" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/LongTermDebtNarrativeDetails" xlink:type="simple" xlink:href="ango-20230228.xsd#LongTermDebtNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/LongTermDebtNarrativeDetails" xlink:type="extended" id="i336eacaf1077454b8907c6ee95093e4f_LongTermDebtNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_8eb14ec1-4b71-4765-b51d-9590601cfc50" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:to="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_8eb14ec1-4b71-4765-b51d-9590601cfc50" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_bc76a8e6-55f7-4219-a8cd-ef67e994fbab" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_bc76a8e6-55f7-4219-a8cd-ef67e994fbab" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfLongTermDebt_6c187824-03f2-48f3-98d2-f644df6de2da" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromIssuanceOfLongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfLongTermDebt_6c187824-03f2-48f3-98d2-f644df6de2da" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_LineOfCreditFacilityMaximumBorrowingCapacityAccordionFeature_308af609-e1f7-418f-89c1-449a4da3af16" xlink:href="ango-20230228.xsd#ango_LineOfCreditFacilityMaximumBorrowingCapacityAccordionFeature"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:to="loc_ango_LineOfCreditFacilityMaximumBorrowingCapacityAccordionFeature_308af609-e1f7-418f-89c1-449a4da3af16" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTerm_1f8c63c4-f172-4bb4-ba39-2313b21bf9ce" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:to="loc_us-gaap_DebtInstrumentTerm_1f8c63c4-f172-4bb4-ba39-2313b21bf9ce" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_799fe03f-eb68-4fe0-b782-0a6873c6a9ff" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_799fe03f-eb68-4fe0-b782-0a6873c6a9ff" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_DebtInstrumentFloorInterestRate_85fdeead-bc58-48d0-83ee-db9b02261f8a" xlink:href="ango-20230228.xsd#ango_DebtInstrumentFloorInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:to="loc_ango_DebtInstrumentFloorInterestRate_85fdeead-bc58-48d0-83ee-db9b02261f8a" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_DebtInstrumentDebtDefaultInterestRateIncrease_1ed8a8c0-a922-43dc-baa9-e56ff7398c1c" xlink:href="ango-20230228.xsd#ango_DebtInstrumentDebtDefaultInterestRateIncrease"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:to="loc_ango_DebtInstrumentDebtDefaultInterestRateIncrease_1ed8a8c0-a922-43dc-baa9-e56ff7398c1c" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityCommitmentFeePercentage_4fac1103-f792-4e6e-86c8-3b8479729c58" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LineOfCreditFacilityCommitmentFeePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:to="loc_us-gaap_LineOfCreditFacilityCommitmentFeePercentage_4fac1103-f792-4e6e-86c8-3b8479729c58" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_LineOfCreditFacilityNumberOfCovenants_09a94908-4eef-4b55-b4ea-9e2b699e3481" xlink:href="ango-20230228.xsd#ango_LineOfCreditFacilityNumberOfCovenants"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:to="loc_ango_LineOfCreditFacilityNumberOfCovenants_09a94908-4eef-4b55-b4ea-9e2b699e3481" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_DebtInstrumentCovenantTermsMaximumTotalLeverageRatio_8f6e6fba-b78d-428b-ac95-840a8d8f873d" xlink:href="ango-20230228.xsd#ango_DebtInstrumentCovenantTermsMaximumTotalLeverageRatio"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:to="loc_ango_DebtInstrumentCovenantTermsMaximumTotalLeverageRatio_8f6e6fba-b78d-428b-ac95-840a8d8f873d" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_DebtInstrumentCovenantTermsMaximumTotalLeverageRatioSubsequentToMaterialAcquisitions_ef7a1678-7ea4-47d1-bfb9-6d779322d70b" xlink:href="ango-20230228.xsd#ango_DebtInstrumentCovenantTermsMaximumTotalLeverageRatioSubsequentToMaterialAcquisitions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:to="loc_ango_DebtInstrumentCovenantTermsMaximumTotalLeverageRatioSubsequentToMaterialAcquisitions_ef7a1678-7ea4-47d1-bfb9-6d779322d70b" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_DebtInstrumentCovenantTermsMinimumFixedChargeCoverageRatio_c88f3e88-d808-4a9d-b0f3-9f15220ef521" xlink:href="ango-20230228.xsd#ango_DebtInstrumentCovenantTermsMinimumFixedChargeCoverageRatio"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:to="loc_ango_DebtInstrumentCovenantTermsMinimumFixedChargeCoverageRatio_c88f3e88-d808-4a9d-b0f3-9f15220ef521" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCredit_6831adc7-dc96-4a0d-82fe-cdb7e724e61e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LineOfCredit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:to="loc_us-gaap_LineOfCredit_6831adc7-dc96-4a0d-82fe-cdb7e724e61e" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_4165b6b1-0ab8-4ded-ae1e-f670df7366de" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:to="loc_us-gaap_LongTermDebt_4165b6b1-0ab8-4ded-ae1e-f670df7366de" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityInterestRateAtPeriodEnd_c42f1e41-443e-4ed0-afdf-1a5bdf863b79" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LineOfCreditFacilityInterestRateAtPeriodEnd"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:to="loc_us-gaap_LineOfCreditFacilityInterestRateAtPeriodEnd_c42f1e41-443e-4ed0-afdf-1a5bdf863b79" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPricePercentage_2b54ddf2-1247-4e5a-99c7-e01cacc756b8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentRedemptionPricePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPricePercentage_2b54ddf2-1247-4e5a-99c7-e01cacc756b8" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_c56cbcc7-dcb7-496a-9cbb-e3d2b160fc97" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_13ecc3c1-bb1c-4c05-a370-511676024fdf" xlink:to="loc_us-gaap_DebtInstrumentTable_c56cbcc7-dcb7-496a-9cbb-e3d2b160fc97" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_563f362c-c5b7-400b-8ef6-5668308c52e0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_c56cbcc7-dcb7-496a-9cbb-e3d2b160fc97" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_563f362c-c5b7-400b-8ef6-5668308c52e0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_563f362c-c5b7-400b-8ef6-5668308c52e0_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_563f362c-c5b7-400b-8ef6-5668308c52e0" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_563f362c-c5b7-400b-8ef6-5668308c52e0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_4d67fde1-4d04-4d68-8143-0c274d99c71d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_563f362c-c5b7-400b-8ef6-5668308c52e0" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_4d67fde1-4d04-4d68-8143-0c274d99c71d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_01bbd7e3-3484-462c-9a3a-4bb5e25d5118" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_4d67fde1-4d04-4d68-8143-0c274d99c71d" xlink:to="loc_us-gaap_LineOfCreditMember_01bbd7e3-3484-462c-9a3a-4bb5e25d5118" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_c9827401-bb20-4d20-8bed-d2b78dbf0bb7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_c56cbcc7-dcb7-496a-9cbb-e3d2b160fc97" xlink:to="loc_us-gaap_CreditFacilityAxis_c9827401-bb20-4d20-8bed-d2b78dbf0bb7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_c9827401-bb20-4d20-8bed-d2b78dbf0bb7_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_c9827401-bb20-4d20-8bed-d2b78dbf0bb7" xlink:to="loc_us-gaap_CreditFacilityDomain_c9827401-bb20-4d20-8bed-d2b78dbf0bb7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_221600f5-edc6-47f7-922d-f474ded00f6c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_c9827401-bb20-4d20-8bed-d2b78dbf0bb7" xlink:to="loc_us-gaap_CreditFacilityDomain_221600f5-edc6-47f7-922d-f474ded00f6c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevolvingCreditFacilityMember_e94abe05-7895-46f6-87a3-242190b5df16" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_221600f5-edc6-47f7-922d-f474ded00f6c" xlink:to="loc_us-gaap_RevolvingCreditFacilityMember_e94abe05-7895-46f6-87a3-242190b5df16" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredDebtMember_598b80d4-4582-409c-ac16-295442620d33" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_221600f5-edc6-47f7-922d-f474ded00f6c" xlink:to="loc_us-gaap_SecuredDebtMember_598b80d4-4582-409c-ac16-295442620d33" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_68a46adf-7cc1-4817-9236-fa11bb455f9d" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_c56cbcc7-dcb7-496a-9cbb-e3d2b160fc97" xlink:to="loc_srt_RangeAxis_68a46adf-7cc1-4817-9236-fa11bb455f9d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_68a46adf-7cc1-4817-9236-fa11bb455f9d_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_68a46adf-7cc1-4817-9236-fa11bb455f9d" xlink:to="loc_srt_RangeMember_68a46adf-7cc1-4817-9236-fa11bb455f9d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_a3440d1c-64f7-4c72-a45a-b9d20f87eb3e" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_68a46adf-7cc1-4817-9236-fa11bb455f9d" xlink:to="loc_srt_RangeMember_a3440d1c-64f7-4c72-a45a-b9d20f87eb3e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_83d88df7-a14d-456c-badc-0870433a1cd9" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_a3440d1c-64f7-4c72-a45a-b9d20f87eb3e" xlink:to="loc_srt_MinimumMember_83d88df7-a14d-456c-badc-0870433a1cd9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_79c999f8-b11d-4628-896c-8e80d1d398c1" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_a3440d1c-64f7-4c72-a45a-b9d20f87eb3e" xlink:to="loc_srt_MaximumMember_79c999f8-b11d-4628-896c-8e80d1d398c1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateAxis_826a5128-dd6f-47f3-8e5d-e924d8778e54" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_VariableRateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_c56cbcc7-dcb7-496a-9cbb-e3d2b160fc97" xlink:to="loc_us-gaap_VariableRateAxis_826a5128-dd6f-47f3-8e5d-e924d8778e54" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_826a5128-dd6f-47f3-8e5d-e924d8778e54_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VariableRateAxis_826a5128-dd6f-47f3-8e5d-e924d8778e54" xlink:to="loc_us-gaap_VariableRateDomain_826a5128-dd6f-47f3-8e5d-e924d8778e54_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_90c94229-616a-40c2-9409-b76855912e02" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VariableRateAxis_826a5128-dd6f-47f3-8e5d-e924d8778e54" xlink:to="loc_us-gaap_VariableRateDomain_90c94229-616a-40c2-9409-b76855912e02" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredOvernightFinancingRateSofrOvernightIndexSwapRateMember_81f622f5-87a4-453f-9993-637373603f4d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SecuredOvernightFinancingRateSofrOvernightIndexSwapRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_90c94229-616a-40c2-9409-b76855912e02" xlink:to="loc_us-gaap_SecuredOvernightFinancingRateSofrOvernightIndexSwapRateMember_81f622f5-87a4-453f-9993-637373603f4d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BaseRateMember_3cd96cc6-7a6a-4865-9880-20f9c01e3af5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_BaseRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_90c94229-616a-40c2-9409-b76855912e02" xlink:to="loc_us-gaap_BaseRateMember_3cd96cc6-7a6a-4865-9880-20f9c01e3af5" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/LongTermDebtScheduleofDebtDetails" xlink:type="simple" xlink:href="ango-20230228.xsd#LongTermDebtScheduleofDebtDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/LongTermDebtScheduleofDebtDetails" xlink:type="extended" id="i88a7db570f574e53b3f20145d531f896_LongTermDebtScheduleofDebtDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_de9794b5-202a-4963-a629-46218e8ba7f9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_d65ee9ae-e196-4748-ae9a-ecc114bf6e91" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_de9794b5-202a-4963-a629-46218e8ba7f9" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_d65ee9ae-e196-4748-ae9a-ecc114bf6e91" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredFinanceCostsNet_47dbeaff-f590-4caa-95f5-8d624b9ee1f8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DeferredFinanceCostsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_de9794b5-202a-4963-a629-46218e8ba7f9" xlink:to="loc_us-gaap_DeferredFinanceCostsNet_47dbeaff-f590-4caa-95f5-8d624b9ee1f8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_d5c9506c-b089-4a98-bf13-9dc93acbcd6c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_de9794b5-202a-4963-a629-46218e8ba7f9" xlink:to="loc_us-gaap_LongTermDebt_d5c9506c-b089-4a98-bf13-9dc93acbcd6c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_11069530-a757-4fc5-a2c3-83ecb73b1c7f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_de9794b5-202a-4963-a629-46218e8ba7f9" xlink:to="loc_us-gaap_DebtInstrumentTable_11069530-a757-4fc5-a2c3-83ecb73b1c7f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_527e9bfa-806e-48a9-9706-0deb89305519" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_11069530-a757-4fc5-a2c3-83ecb73b1c7f" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_527e9bfa-806e-48a9-9706-0deb89305519" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_527e9bfa-806e-48a9-9706-0deb89305519_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_527e9bfa-806e-48a9-9706-0deb89305519" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_527e9bfa-806e-48a9-9706-0deb89305519_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_196e40da-5128-43a9-b890-0c987332c2b0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_527e9bfa-806e-48a9-9706-0deb89305519" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_196e40da-5128-43a9-b890-0c987332c2b0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_e663c7e4-3260-4545-ad85-fa886b2db2f6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_196e40da-5128-43a9-b890-0c987332c2b0" xlink:to="loc_us-gaap_LineOfCreditMember_e663c7e4-3260-4545-ad85-fa886b2db2f6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_2978834d-1ea9-4136-afb8-55b52918b377" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_11069530-a757-4fc5-a2c3-83ecb73b1c7f" xlink:to="loc_us-gaap_CreditFacilityAxis_2978834d-1ea9-4136-afb8-55b52918b377" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_2978834d-1ea9-4136-afb8-55b52918b377_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_2978834d-1ea9-4136-afb8-55b52918b377" xlink:to="loc_us-gaap_CreditFacilityDomain_2978834d-1ea9-4136-afb8-55b52918b377_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_04eac791-a0f6-413e-bb38-489b55acbfde" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_2978834d-1ea9-4136-afb8-55b52918b377" xlink:to="loc_us-gaap_CreditFacilityDomain_04eac791-a0f6-413e-bb38-489b55acbfde" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevolvingCreditFacilityMember_235373ea-1d96-42bd-8013-92a9c1d7f729" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_04eac791-a0f6-413e-bb38-489b55acbfde" xlink:to="loc_us-gaap_RevolvingCreditFacilityMember_235373ea-1d96-42bd-8013-92a9c1d7f729" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredDebtMember_92d1ddaf-22f4-44d6-8712-93bc503f7ea0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_04eac791-a0f6-413e-bb38-489b55acbfde" xlink:to="loc_us-gaap_SecuredDebtMember_92d1ddaf-22f4-44d6-8712-93bc503f7ea0" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/ShareBasedCompensationDetails" xlink:type="simple" xlink:href="ango-20230228.xsd#ShareBasedCompensationDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/ShareBasedCompensationDetails" xlink:type="extended" id="i4a769711ecea48c1b83e02772db5beb8_ShareBasedCompensationDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_3f1a653f-9f07-4f16-b846-a11ef381d9c4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingPeriodIncreaseDecrease_a98e3c76-6005-4660-8a2a-d23d300b6377" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingPeriodIncreaseDecrease"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_3f1a653f-9f07-4f16-b846-a11ef381d9c4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingPeriodIncreaseDecrease_a98e3c76-6005-4660-8a2a-d23d300b6377" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized_2b218710-936d-4239-a9fd-3390e3e8e061" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_3f1a653f-9f07-4f16-b846-a11ef381d9c4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized_2b218710-936d-4239-a9fd-3390e3e8e061" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_65bb0c63-e7c0-462b-88b9-f9da7c93b4f3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_3f1a653f-9f07-4f16-b846-a11ef381d9c4" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_65bb0c63-e7c0-462b-88b9-f9da7c93b4f3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_81c16cbd-0e71-4ab1-8f7b-171b9144cfb9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_3f1a653f-9f07-4f16-b846-a11ef381d9c4" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_81c16cbd-0e71-4ab1-8f7b-171b9144cfb9" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_72353fa2-8dfe-4c09-8a03-9e809aa4fdbb" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_3f1a653f-9f07-4f16-b846-a11ef381d9c4" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_72353fa2-8dfe-4c09-8a03-9e809aa4fdbb" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_534f4f90-2b25-44ba-85ff-18e26748261d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_3f1a653f-9f07-4f16-b846-a11ef381d9c4" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_534f4f90-2b25-44ba-85ff-18e26748261d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_125fbfe1-67f0-40d0-905d-d0dd1f5e862c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_534f4f90-2b25-44ba-85ff-18e26748261d" xlink:to="loc_us-gaap_PlanNameAxis_125fbfe1-67f0-40d0-905d-d0dd1f5e862c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_125fbfe1-67f0-40d0-905d-d0dd1f5e862c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_125fbfe1-67f0-40d0-905d-d0dd1f5e862c" xlink:to="loc_us-gaap_PlanNameDomain_125fbfe1-67f0-40d0-905d-d0dd1f5e862c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_e975cdf4-d192-47f5-ba3d-1b7d680a4def" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_125fbfe1-67f0-40d0-905d-d0dd1f5e862c" xlink:to="loc_us-gaap_PlanNameDomain_e975cdf4-d192-47f5-ba3d-1b7d680a4def" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_A2020StockAndIncentiveAwardPlanMember_fece9b4a-bab1-4069-b548-2e6964f3103d" xlink:href="ango-20230228.xsd#ango_A2020StockAndIncentiveAwardPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_e975cdf4-d192-47f5-ba3d-1b7d680a4def" xlink:to="loc_ango_A2020StockAndIncentiveAwardPlanMember_fece9b4a-bab1-4069-b548-2e6964f3103d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_EmployeeStockPurchasePlanMember_21457fab-074e-44eb-a084-50aee0e4c7e8" xlink:href="ango-20230228.xsd#ango_EmployeeStockPurchasePlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_e975cdf4-d192-47f5-ba3d-1b7d680a4def" xlink:to="loc_ango_EmployeeStockPurchasePlanMember_21457fab-074e-44eb-a084-50aee0e4c7e8" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/SegmentandGeographicInformationNarrativeDetails" xlink:type="simple" xlink:href="ango-20230228.xsd#SegmentandGeographicInformationNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/SegmentandGeographicInformationNarrativeDetails" xlink:type="extended" id="i8791ebd3589c4859a786ddc18ab8e38c_SegmentandGeographicInformationNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_64815d9e-b8a3-4343-b7c0-8a0ab426ae10" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfOperatingSegments_c371729d-455e-4a99-99c2-074a37a835b3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NumberOfOperatingSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_64815d9e-b8a3-4343-b7c0-8a0ab426ae10" xlink:to="loc_us-gaap_NumberOfOperatingSegments_c371729d-455e-4a99-99c2-074a37a835b3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_99395dfa-e561-43fc-8346-52d1321116d6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_Assets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_64815d9e-b8a3-4343-b7c0-8a0ab426ae10" xlink:to="loc_us-gaap_Assets_99395dfa-e561-43fc-8346-52d1321116d6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskPercentage1_6212c995-6ba6-4621-b904-f6659f679501" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConcentrationRiskPercentage1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_64815d9e-b8a3-4343-b7c0-8a0ab426ae10" xlink:to="loc_us-gaap_ConcentrationRiskPercentage1_6212c995-6ba6-4621-b904-f6659f679501" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_eb72a07e-1853-4d38-af2a-4cee887031d8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_64815d9e-b8a3-4343-b7c0-8a0ab426ae10" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_eb72a07e-1853-4d38-af2a-4cee887031d8" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_a8d72567-e0be-4967-986f-c4f9904007f9" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_eb72a07e-1853-4d38-af2a-4cee887031d8" xlink:to="loc_srt_StatementGeographicalAxis_a8d72567-e0be-4967-986f-c4f9904007f9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_a8d72567-e0be-4967-986f-c4f9904007f9_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_a8d72567-e0be-4967-986f-c4f9904007f9" xlink:to="loc_srt_SegmentGeographicalDomain_a8d72567-e0be-4967-986f-c4f9904007f9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_52f0b170-7ff5-4fdc-860b-1bf4a281611e" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_a8d72567-e0be-4967-986f-c4f9904007f9" xlink:to="loc_srt_SegmentGeographicalDomain_52f0b170-7ff5-4fdc-860b-1bf4a281611e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonUsMember_4a3235a8-35a6-4445-b87c-f75bd8f3fb0f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NonUsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_52f0b170-7ff5-4fdc-860b-1bf4a281611e" xlink:to="loc_us-gaap_NonUsMember_4a3235a8-35a6-4445-b87c-f75bd8f3fb0f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_cec90e31-19fa-422e-b67e-65dae3496af5" xlink:href="https://xbrl.sec.gov/country/2021/country-2021.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_52f0b170-7ff5-4fdc-860b-1bf4a281611e" xlink:to="loc_country_US_cec90e31-19fa-422e-b67e-65dae3496af5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_cf46f417-3096-4dc2-b255-9d2fb349e92e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConcentrationRiskByBenchmarkAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_eb72a07e-1853-4d38-af2a-4cee887031d8" xlink:to="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_cf46f417-3096-4dc2-b255-9d2fb349e92e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_cf46f417-3096-4dc2-b255-9d2fb349e92e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_cf46f417-3096-4dc2-b255-9d2fb349e92e" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_cf46f417-3096-4dc2-b255-9d2fb349e92e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_ac61ce07-88f0-445e-86d4-d07104e5ef70" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_cf46f417-3096-4dc2-b255-9d2fb349e92e" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_ac61ce07-88f0-445e-86d4-d07104e5ef70" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerMember_c9286a03-d72d-4d73-8ba4-635d3762b7a4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenueFromContractWithCustomerMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_ac61ce07-88f0-445e-86d4-d07104e5ef70" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerMember_c9286a03-d72d-4d73-8ba4-635d3762b7a4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetAssetsGeographicAreaMember_1bbee48e-4929-47d1-976f-c5edc4ebf031" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetAssetsGeographicAreaMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_ac61ce07-88f0-445e-86d4-d07104e5ef70" xlink:to="loc_us-gaap_NetAssetsGeographicAreaMember_1bbee48e-4929-47d1-976f-c5edc4ebf031" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByTypeAxis_b8f868a8-ee56-428f-8da9-5bd5718dbe18" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConcentrationRiskByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_eb72a07e-1853-4d38-af2a-4cee887031d8" xlink:to="loc_us-gaap_ConcentrationRiskByTypeAxis_b8f868a8-ee56-428f-8da9-5bd5718dbe18" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_b8f868a8-ee56-428f-8da9-5bd5718dbe18_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_b8f868a8-ee56-428f-8da9-5bd5718dbe18" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_b8f868a8-ee56-428f-8da9-5bd5718dbe18_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_945ac2e3-7533-407d-a346-1796fd06efc0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_b8f868a8-ee56-428f-8da9-5bd5718dbe18" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_945ac2e3-7533-407d-a346-1796fd06efc0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeographicConcentrationRiskMember_b61df0a6-6bfa-4f31-a439-73a8640847b9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_GeographicConcentrationRiskMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskTypeDomain_945ac2e3-7533-407d-a346-1796fd06efc0" xlink:to="loc_us-gaap_GeographicConcentrationRiskMember_b61df0a6-6bfa-4f31-a439-73a8640847b9" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/SegmentandGeographicInformationSummaryofNetSalesbyProductCategoryDetails" xlink:type="simple" xlink:href="ango-20230228.xsd#SegmentandGeographicInformationSummaryofNetSalesbyProductCategoryDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/SegmentandGeographicInformationSummaryofNetSalesbyProductCategoryDetails" xlink:type="extended" id="i9b25683207ca4bafb5f7ae6c22a2f41e_SegmentandGeographicInformationSummaryofNetSalesbyProductCategoryDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_c2761748-87e1-43cf-bf56-3dfe4ba8e6b0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_23397aaa-f446-4796-a96d-678b8f4101d5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_c2761748-87e1-43cf-bf56-3dfe4ba8e6b0" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_23397aaa-f446-4796-a96d-678b8f4101d5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GrossProfit_b70f6730-0bd0-4264-abf4-f0394248d562" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_GrossProfit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_c2761748-87e1-43cf-bf56-3dfe4ba8e6b0" xlink:to="loc_us-gaap_GrossProfit_b70f6730-0bd0-4264-abf4-f0394248d562" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_GrossMarginPercentage_8158c3a8-ee5a-4b86-a1f4-472ff376cb31" xlink:href="ango-20230228.xsd#ango_GrossMarginPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_c2761748-87e1-43cf-bf56-3dfe4ba8e6b0" xlink:to="loc_ango_GrossMarginPercentage_8158c3a8-ee5a-4b86-a1f4-472ff376cb31" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_973c081f-0e0f-45c6-b8fd-7f631c019c0d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_c2761748-87e1-43cf-bf56-3dfe4ba8e6b0" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_973c081f-0e0f-45c6-b8fd-7f631c019c0d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_faa797f0-be79-42ec-9314-e638e41b0c71" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_973c081f-0e0f-45c6-b8fd-7f631c019c0d" xlink:to="loc_srt_ProductOrServiceAxis_faa797f0-be79-42ec-9314-e638e41b0c71" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_faa797f0-be79-42ec-9314-e638e41b0c71_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_faa797f0-be79-42ec-9314-e638e41b0c71" xlink:to="loc_srt_ProductsAndServicesDomain_faa797f0-be79-42ec-9314-e638e41b0c71_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_3d61263e-f295-4302-8c9f-16ab731dae1a" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_faa797f0-be79-42ec-9314-e638e41b0c71" xlink:to="loc_srt_ProductsAndServicesDomain_3d61263e-f295-4302-8c9f-16ab731dae1a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_MedTechMember_26a3afcf-ad19-4cd9-83fe-71b8ad7d2162" xlink:href="ango-20230228.xsd#ango_MedTechMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_3d61263e-f295-4302-8c9f-16ab731dae1a" xlink:to="loc_ango_MedTechMember_26a3afcf-ad19-4cd9-83fe-71b8ad7d2162" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_MedDeviceMember_fd1abfb1-78a6-4824-9284-dd49392e66ad" xlink:href="ango-20230228.xsd#ango_MedDeviceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_3d61263e-f295-4302-8c9f-16ab731dae1a" xlink:to="loc_ango_MedDeviceMember_fd1abfb1-78a6-4824-9284-dd49392e66ad" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_20b49f8c-1261-47f6-8f8c-88f6fe46cf2e" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_973c081f-0e0f-45c6-b8fd-7f631c019c0d" xlink:to="loc_srt_StatementGeographicalAxis_20b49f8c-1261-47f6-8f8c-88f6fe46cf2e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_20b49f8c-1261-47f6-8f8c-88f6fe46cf2e_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_20b49f8c-1261-47f6-8f8c-88f6fe46cf2e" xlink:to="loc_srt_SegmentGeographicalDomain_20b49f8c-1261-47f6-8f8c-88f6fe46cf2e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_66f2d620-7d01-4d08-825e-9c1c5d35f3bb" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_20b49f8c-1261-47f6-8f8c-88f6fe46cf2e" xlink:to="loc_srt_SegmentGeographicalDomain_66f2d620-7d01-4d08-825e-9c1c5d35f3bb" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/SegmentandGeographicInformationSummaryofNetSalesbyGeographicAreaDetails" xlink:type="simple" xlink:href="ango-20230228.xsd#SegmentandGeographicInformationSummaryofNetSalesbyGeographicAreaDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/SegmentandGeographicInformationSummaryofNetSalesbyGeographicAreaDetails" xlink:type="extended" id="i8fc7815036554d93a8379da4b80f2e7d_SegmentandGeographicInformationSummaryofNetSalesbyGeographicAreaDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_347b656b-917b-4d66-9bc0-b825308dc7dc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_6f3dc0c3-bc7f-457c-8d91-29bb46abdb3d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_347b656b-917b-4d66-9bc0-b825308dc7dc" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_6f3dc0c3-bc7f-457c-8d91-29bb46abdb3d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_6c811110-4cef-43fb-9187-8021d18b26ff" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_347b656b-917b-4d66-9bc0-b825308dc7dc" xlink:to="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_6c811110-4cef-43fb-9187-8021d18b26ff" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_5a0ad1ea-b7e2-4a83-9f34-df9cd81c0305" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_6c811110-4cef-43fb-9187-8021d18b26ff" xlink:to="loc_srt_StatementGeographicalAxis_5a0ad1ea-b7e2-4a83-9f34-df9cd81c0305" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_5a0ad1ea-b7e2-4a83-9f34-df9cd81c0305_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_5a0ad1ea-b7e2-4a83-9f34-df9cd81c0305" xlink:to="loc_srt_SegmentGeographicalDomain_5a0ad1ea-b7e2-4a83-9f34-df9cd81c0305_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_93c970a7-eba6-49d0-b039-ddb183c6d2f0" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_5a0ad1ea-b7e2-4a83-9f34-df9cd81c0305" xlink:to="loc_srt_SegmentGeographicalDomain_93c970a7-eba6-49d0-b039-ddb183c6d2f0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_98958be0-a838-423c-90b2-3f19c8b5ddf1" xlink:href="https://xbrl.sec.gov/country/2021/country-2021.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_93c970a7-eba6-49d0-b039-ddb183c6d2f0" xlink:to="loc_country_US_98958be0-a838-423c-90b2-3f19c8b5ddf1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonUsMember_a28a1345-de41-4ed4-8066-cac76e977d3c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NonUsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_93c970a7-eba6-49d0-b039-ddb183c6d2f0" xlink:to="loc_us-gaap_NonUsMember_a28a1345-de41-4ed4-8066-cac76e977d3c" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/FairValueFairValueofAssetsandLiabilitiesMeasuredonaRecurringBasisDetails" xlink:type="simple" xlink:href="ango-20230228.xsd#FairValueFairValueofAssetsandLiabilitiesMeasuredonaRecurringBasisDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/FairValueFairValueofAssetsandLiabilitiesMeasuredonaRecurringBasisDetails" xlink:type="extended" id="i30399458c1d04a43a39f7d7e682bdf6d_FairValueFairValueofAssetsandLiabilitiesMeasuredonaRecurringBasisDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_69aadde5-f61f-4e2f-992d-4a920910e47d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_76888cb8-da6d-445e-ab59-fb70bf09e29c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LiabilitiesFairValueDisclosureAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_69aadde5-f61f-4e2f-992d-4a920910e47d" xlink:to="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_76888cb8-da6d-445e-ab59-fb70bf09e29c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_8eedecea-9001-427d-a306-3b15ac8d6e19" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_BusinessCombinationContingentConsiderationLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_76888cb8-da6d-445e-ab59-fb70bf09e29c" xlink:to="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_8eedecea-9001-427d-a306-3b15ac8d6e19" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesFairValueDisclosure_b415575f-6d38-4283-b9b0-12487ec69c72" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LiabilitiesFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_76888cb8-da6d-445e-ab59-fb70bf09e29c" xlink:to="loc_us-gaap_LiabilitiesFairValueDisclosure_b415575f-6d38-4283-b9b0-12487ec69c72" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_dfe36653-dda5-4504-aa0b-c3b72a1c96c6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_69aadde5-f61f-4e2f-992d-4a920910e47d" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_dfe36653-dda5-4504-aa0b-c3b72a1c96c6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_877d4a9b-5c35-43e0-aae4-16ea4495e5f8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_dfe36653-dda5-4504-aa0b-c3b72a1c96c6" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_877d4a9b-5c35-43e0-aae4-16ea4495e5f8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_877d4a9b-5c35-43e0-aae4-16ea4495e5f8_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_877d4a9b-5c35-43e0-aae4-16ea4495e5f8" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_877d4a9b-5c35-43e0-aae4-16ea4495e5f8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_3e044763-1e35-4e37-899d-acfec4540fc9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_877d4a9b-5c35-43e0-aae4-16ea4495e5f8" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_3e044763-1e35-4e37-899d-acfec4540fc9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_15c8b2d0-f03c-4396-b889-39344c430619" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_3e044763-1e35-4e37-899d-acfec4540fc9" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_15c8b2d0-f03c-4396-b889-39344c430619" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_4e06210c-d289-46cf-b565-b3714c77ca10" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_3e044763-1e35-4e37-899d-acfec4540fc9" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_4e06210c-d289-46cf-b565-b3714c77ca10" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_59768735-b386-4d10-99cf-9c188c360aec" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_3e044763-1e35-4e37-899d-acfec4540fc9" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_59768735-b386-4d10-99cf-9c188c360aec" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_1c3f83a7-698d-4d1e-9836-0fc52212e6c4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_dfe36653-dda5-4504-aa0b-c3b72a1c96c6" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_1c3f83a7-698d-4d1e-9836-0fc52212e6c4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_1c3f83a7-698d-4d1e-9836-0fc52212e6c4_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_1c3f83a7-698d-4d1e-9836-0fc52212e6c4" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_1c3f83a7-698d-4d1e-9836-0fc52212e6c4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_edd8bb6e-af2b-4393-937d-8868b5482f75" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_1c3f83a7-698d-4d1e-9836-0fc52212e6c4" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_edd8bb6e-af2b-4393-937d-8868b5482f75" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsRecurringMember_8a3b60a2-02d4-4cc3-bf02-51b9f5e5d7ac" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementsRecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_edd8bb6e-af2b-4393-937d-8868b5482f75" xlink:to="loc_us-gaap_FairValueMeasurementsRecurringMember_8a3b60a2-02d4-4cc3-bf02-51b9f5e5d7ac" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/FairValueSummaryShowingtheRecurringFairValueMeasurementsoftheContingentConsiderationLiabilityDetails" xlink:type="simple" xlink:href="ango-20230228.xsd#FairValueSummaryShowingtheRecurringFairValueMeasurementsoftheContingentConsiderationLiabilityDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/FairValueSummaryShowingtheRecurringFairValueMeasurementsoftheContingentConsiderationLiabilityDetails" xlink:type="extended" id="i6166fa8f1a354a9d960a55a5e236446c_FairValueSummaryShowingtheRecurringFairValueMeasurementsoftheContingentConsiderationLiabilityDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_8ce6ee5b-fbcf-414a-b1ae-585ca892778f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_771743b7-b08b-4c52-bcb3-f29b3992d004" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_BusinessCombinationContingentConsiderationLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_8ce6ee5b-fbcf-414a-b1ae-585ca892778f" xlink:to="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_771743b7-b08b-4c52-bcb3-f29b3992d004" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationContingentConsiderationLiabilityMeasurementInput_6fd731f9-5251-4722-b069-34216bb69f87" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_BusinessCombinationContingentConsiderationLiabilityMeasurementInput"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_8ce6ee5b-fbcf-414a-b1ae-585ca892778f" xlink:to="loc_us-gaap_BusinessCombinationContingentConsiderationLiabilityMeasurementInput_6fd731f9-5251-4722-b069-34216bb69f87" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_b1ccd88a-e9ac-4c77-b73b-64472d216c08" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_8ce6ee5b-fbcf-414a-b1ae-585ca892778f" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_b1ccd88a-e9ac-4c77-b73b-64472d216c08" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeAxis_f482fe0b-e7eb-41f9-be86-79d5705b2b91" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_MeasurementInputTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_b1ccd88a-e9ac-4c77-b73b-64472d216c08" xlink:to="loc_us-gaap_MeasurementInputTypeAxis_f482fe0b-e7eb-41f9-be86-79d5705b2b91" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeDomain_f482fe0b-e7eb-41f9-be86-79d5705b2b91_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_MeasurementInputTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_MeasurementInputTypeAxis_f482fe0b-e7eb-41f9-be86-79d5705b2b91" xlink:to="loc_us-gaap_MeasurementInputTypeDomain_f482fe0b-e7eb-41f9-be86-79d5705b2b91_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeDomain_2e84dd47-8868-4f96-8945-708d2b1dc64d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_MeasurementInputTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_MeasurementInputTypeAxis_f482fe0b-e7eb-41f9-be86-79d5705b2b91" xlink:to="loc_us-gaap_MeasurementInputTypeDomain_2e84dd47-8868-4f96-8945-708d2b1dc64d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputDiscountRateMember_b293c403-0f9d-45c4-9712-e1ed2cede279" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_MeasurementInputDiscountRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MeasurementInputTypeDomain_2e84dd47-8868-4f96-8945-708d2b1dc64d" xlink:to="loc_us-gaap_MeasurementInputDiscountRateMember_b293c403-0f9d-45c4-9712-e1ed2cede279" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_MeasurementInputProbabilityofPaymentMember_155a8d0a-7c54-4473-abe8-635326b6aaa1" xlink:href="ango-20230228.xsd#ango_MeasurementInputProbabilityofPaymentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MeasurementInputTypeDomain_2e84dd47-8868-4f96-8945-708d2b1dc64d" xlink:to="loc_ango_MeasurementInputProbabilityofPaymentMember_155a8d0a-7c54-4473-abe8-635326b6aaa1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContingentConsiderationByTypeAxis_b678cd15-9493-4ab8-aafb-bfc1b625a98b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ContingentConsiderationByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_b1ccd88a-e9ac-4c77-b73b-64472d216c08" xlink:to="loc_us-gaap_ContingentConsiderationByTypeAxis_b678cd15-9493-4ab8-aafb-bfc1b625a98b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContingentConsiderationTypeDomain_b678cd15-9493-4ab8-aafb-bfc1b625a98b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ContingentConsiderationTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ContingentConsiderationByTypeAxis_b678cd15-9493-4ab8-aafb-bfc1b625a98b" xlink:to="loc_us-gaap_ContingentConsiderationTypeDomain_b678cd15-9493-4ab8-aafb-bfc1b625a98b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContingentConsiderationTypeDomain_613b7d5a-662c-43f5-8ca3-e693e208e50d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ContingentConsiderationTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ContingentConsiderationByTypeAxis_b678cd15-9493-4ab8-aafb-bfc1b625a98b" xlink:to="loc_us-gaap_ContingentConsiderationTypeDomain_613b7d5a-662c-43f5-8ca3-e693e208e50d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_RevenueBasedPaymentMember_8cf03f57-47d2-45cf-ba3c-ce1e4c07fdcc" xlink:href="ango-20230228.xsd#ango_RevenueBasedPaymentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContingentConsiderationTypeDomain_613b7d5a-662c-43f5-8ca3-e693e208e50d" xlink:to="loc_ango_RevenueBasedPaymentMember_8cf03f57-47d2-45cf-ba3c-ce1e4c07fdcc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_410dbedf-8cca-493a-a3eb-a78bbe8a1182" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_b1ccd88a-e9ac-4c77-b73b-64472d216c08" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_410dbedf-8cca-493a-a3eb-a78bbe8a1182" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_410dbedf-8cca-493a-a3eb-a78bbe8a1182_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_410dbedf-8cca-493a-a3eb-a78bbe8a1182" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_410dbedf-8cca-493a-a3eb-a78bbe8a1182_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_87ce04e2-81b5-4aed-8b92-f8d8f053940a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_410dbedf-8cca-493a-a3eb-a78bbe8a1182" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_87ce04e2-81b5-4aed-8b92-f8d8f053940a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_85da3f07-41b7-43e0-a383-f404a798bc74" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_87ce04e2-81b5-4aed-8b92-f8d8f053940a" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_85da3f07-41b7-43e0-a383-f404a798bc74" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_46b43375-e933-48ce-90b8-9f048f712c82" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_b1ccd88a-e9ac-4c77-b73b-64472d216c08" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_46b43375-e933-48ce-90b8-9f048f712c82" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_46b43375-e933-48ce-90b8-9f048f712c82_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_46b43375-e933-48ce-90b8-9f048f712c82" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_46b43375-e933-48ce-90b8-9f048f712c82_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_738fa32e-f0a0-4277-a785-ebf5026407e3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_46b43375-e933-48ce-90b8-9f048f712c82" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_738fa32e-f0a0-4277-a785-ebf5026407e3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsRecurringMember_3279e3aa-a02e-43c2-9e90-66be1384baca" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementsRecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_738fa32e-f0a0-4277-a785-ebf5026407e3" xlink:to="loc_us-gaap_FairValueMeasurementsRecurringMember_3279e3aa-a02e-43c2-9e90-66be1384baca" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationTechniqueAxis_654db534-7905-4fb0-9f3b-0592b829d1d5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ValuationTechniqueAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_b1ccd88a-e9ac-4c77-b73b-64472d216c08" xlink:to="loc_us-gaap_ValuationTechniqueAxis_654db534-7905-4fb0-9f3b-0592b829d1d5" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationTechniqueDomain_654db534-7905-4fb0-9f3b-0592b829d1d5_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ValuationTechniqueDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ValuationTechniqueAxis_654db534-7905-4fb0-9f3b-0592b829d1d5" xlink:to="loc_us-gaap_ValuationTechniqueDomain_654db534-7905-4fb0-9f3b-0592b829d1d5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationTechniqueDomain_b03f1e94-eb29-4c7d-9750-483243c54945" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ValuationTechniqueDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ValuationTechniqueAxis_654db534-7905-4fb0-9f3b-0592b829d1d5" xlink:to="loc_us-gaap_ValuationTechniqueDomain_b03f1e94-eb29-4c7d-9750-483243c54945" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationTechniqueDiscountedCashFlowMember_08320435-60ab-4019-abcb-e7dc9500190b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ValuationTechniqueDiscountedCashFlowMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ValuationTechniqueDomain_b03f1e94-eb29-4c7d-9750-483243c54945" xlink:to="loc_us-gaap_ValuationTechniqueDiscountedCashFlowMember_08320435-60ab-4019-abcb-e7dc9500190b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_86b8470a-a79d-431c-9b8b-d2aff5ffdc1c" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_b1ccd88a-e9ac-4c77-b73b-64472d216c08" xlink:to="loc_srt_RangeAxis_86b8470a-a79d-431c-9b8b-d2aff5ffdc1c" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_86b8470a-a79d-431c-9b8b-d2aff5ffdc1c_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_86b8470a-a79d-431c-9b8b-d2aff5ffdc1c" xlink:to="loc_srt_RangeMember_86b8470a-a79d-431c-9b8b-d2aff5ffdc1c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_2b7a689f-0ab5-4bef-9174-46371f307961" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_86b8470a-a79d-431c-9b8b-d2aff5ffdc1c" xlink:to="loc_srt_RangeMember_2b7a689f-0ab5-4bef-9174-46371f307961" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_da8a4332-f9d2-45e5-a970-cbe3523fa237" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_2b7a689f-0ab5-4bef-9174-46371f307961" xlink:to="loc_srt_MinimumMember_da8a4332-f9d2-45e5-a970-cbe3523fa237" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_52b873ba-30f3-496e-b7b8-34b297b11496" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_2b7a689f-0ab5-4bef-9174-46371f307961" xlink:to="loc_srt_MaximumMember_52b873ba-30f3-496e-b7b8-34b297b11496" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/LeasesSupplementalStatementofOperationsInformationDetails" xlink:type="simple" xlink:href="ango-20230228.xsd#LeasesSupplementalStatementofOperationsInformationDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/LeasesSupplementalStatementofOperationsInformationDetails" xlink:type="extended" id="i58e887c25d5642b6ab2a38eab13c9814_LeasesSupplementalStatementofOperationsInformationDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeLeaseDescriptionLineItems_af284d0e-805e-4e99-9835-b9ea25ec9997" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeLeaseDescriptionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseExpense_6143de24-39ca-4e37-8786-720a04ac1daa" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeaseExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_af284d0e-805e-4e99-9835-b9ea25ec9997" xlink:to="loc_us-gaap_OperatingLeaseExpense_6143de24-39ca-4e37-8786-720a04ac1daa" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeLeaseDescriptionTable_c1d60a92-0257-41cc-8a34-c3a54d60c4ba" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeLeaseDescriptionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_af284d0e-805e-4e99-9835-b9ea25ec9997" xlink:to="loc_us-gaap_LesseeLeaseDescriptionTable_c1d60a92-0257-41cc-8a34-c3a54d60c4ba" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_d47a0291-2320-4996-8a27-f2ddce887777" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LesseeLeaseDescriptionTable_c1d60a92-0257-41cc-8a34-c3a54d60c4ba" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_d47a0291-2320-4996-8a27-f2ddce887777" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_d47a0291-2320-4996-8a27-f2ddce887777_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_d47a0291-2320-4996-8a27-f2ddce887777" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_d47a0291-2320-4996-8a27-f2ddce887777_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_a46eec09-55ae-47fc-bafb-5bdac6eeab1d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_d47a0291-2320-4996-8a27-f2ddce887777" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_a46eec09-55ae-47fc-bafb-5bdac6eeab1d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfSalesMember_c3d7e9af-20d9-433c-823f-3e0f78665223" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CostOfSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_a46eec09-55ae-47fc-bafb-5bdac6eeab1d" xlink:to="loc_us-gaap_CostOfSalesMember_c3d7e9af-20d9-433c-823f-3e0f78665223" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpenseMember_2b55fad3-36ab-4dd7-b33b-086ecac48175" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ResearchAndDevelopmentExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_a46eec09-55ae-47fc-bafb-5bdac6eeab1d" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpenseMember_2b55fad3-36ab-4dd7-b33b-086ecac48175" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingAndMarketingExpenseMember_d2dad757-5e6a-4520-b6aa-16b86c1ca5c9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SellingAndMarketingExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_a46eec09-55ae-47fc-bafb-5bdac6eeab1d" xlink:to="loc_us-gaap_SellingAndMarketingExpenseMember_d2dad757-5e6a-4520-b6aa-16b86c1ca5c9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpenseMember_205b2399-c2e6-48d5-a516-76fd89f21e7d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_GeneralAndAdministrativeExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_a46eec09-55ae-47fc-bafb-5bdac6eeab1d" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpenseMember_205b2399-c2e6-48d5-a516-76fd89f21e7d" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/CommitmentsandContingenciesDetails" xlink:type="simple" xlink:href="ango-20230228.xsd#CommitmentsandContingenciesDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/CommitmentsandContingenciesDetails" xlink:type="extended" id="ie92c36de7d2f4d28aaa77c617c11737f_CommitmentsandContingenciesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingenciesLineItems_886af484-d60a-41e2-b00b-daff8d4d3267" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LossContingenciesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_NumberOfPatentsAssertedForReexamination_c72d369c-d4cd-4372-a79d-d56a63d5614b" xlink:href="ango-20230228.xsd#ango_NumberOfPatentsAssertedForReexamination"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_886af484-d60a-41e2-b00b-daff8d4d3267" xlink:to="loc_ango_NumberOfPatentsAssertedForReexamination_c72d369c-d4cd-4372-a79d-d56a63d5614b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_NumberOfPatentsUpheldOverPriorArtReferences_99dd7c3b-83c4-475d-a214-b3f560611098" xlink:href="ango-20230228.xsd#ango_NumberOfPatentsUpheldOverPriorArtReferences"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_886af484-d60a-41e2-b00b-daff8d4d3267" xlink:to="loc_ango_NumberOfPatentsUpheldOverPriorArtReferences_99dd7c3b-83c4-475d-a214-b3f560611098" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyPatentsAllegedlyInfringedNumber_2bb6e8d9-a250-4580-970d-f1403521ba82" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LossContingencyPatentsAllegedlyInfringedNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_886af484-d60a-41e2-b00b-daff8d4d3267" xlink:to="loc_us-gaap_LossContingencyPatentsAllegedlyInfringedNumber_2bb6e8d9-a250-4580-970d-f1403521ba82" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingenciesTable_f0ae2095-5507-432f-8fbd-d5e7fdbb5723" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LossContingenciesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LossContingenciesLineItems_886af484-d60a-41e2-b00b-daff8d4d3267" xlink:to="loc_us-gaap_LossContingenciesTable_f0ae2095-5507-432f-8fbd-d5e7fdbb5723" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_LitigationCaseAxis_6f9a757c-560e-4065-893f-744dcce04043" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_LitigationCaseAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LossContingenciesTable_f0ae2095-5507-432f-8fbd-d5e7fdbb5723" xlink:to="loc_srt_LitigationCaseAxis_6f9a757c-560e-4065-893f-744dcce04043" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_LitigationCaseTypeDomain_6f9a757c-560e-4065-893f-744dcce04043_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_LitigationCaseTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_LitigationCaseAxis_6f9a757c-560e-4065-893f-744dcce04043" xlink:to="loc_srt_LitigationCaseTypeDomain_6f9a757c-560e-4065-893f-744dcce04043_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_LitigationCaseTypeDomain_6791d7e0-a32f-4b2d-8db3-25f465075cb2" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_LitigationCaseTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_LitigationCaseAxis_6f9a757c-560e-4065-893f-744dcce04043" xlink:to="loc_srt_LitigationCaseTypeDomain_6791d7e0-a32f-4b2d-8db3-25f465075cb2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_TheDelawareActionMember_85c3b242-1737-4216-a81f-37aca784e2d8" xlink:href="ango-20230228.xsd#ango_TheDelawareActionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_LitigationCaseTypeDomain_6791d7e0-a32f-4b2d-8db3-25f465075cb2" xlink:to="loc_ango_TheDelawareActionMember_85c3b242-1737-4216-a81f-37aca784e2d8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_CrBardIncMember_14705d87-ff6b-4045-b5d7-079cb5980043" xlink:href="ango-20230228.xsd#ango_CrBardIncMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_LitigationCaseTypeDomain_6791d7e0-a32f-4b2d-8db3-25f465075cb2" xlink:to="loc_ango_CrBardIncMember_14705d87-ff6b-4045-b5d7-079cb5980043" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AcquisitionRestructuringandOtherItemsNetDetails" xlink:type="simple" xlink:href="ango-20230228.xsd#AcquisitionRestructuringandOtherItemsNetDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AcquisitionRestructuringandOtherItemsNetDetails" xlink:type="extended" id="iaf254b25997a4d7cacd7bcab6bf376e6_AcquisitionRestructuringandOtherItemsNetDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringCostAndReserveLineItems_c77246b2-da65-48be-99cb-b82293cc22b4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RestructuringCostAndReserveLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_AcquisitionRestructuringAndOtherItemsNet_dab627b7-12fc-4f03-bd3f-15bd67fc077a" xlink:href="ango-20230228.xsd#ango_AcquisitionRestructuringAndOtherItemsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_c77246b2-da65-48be-99cb-b82293cc22b4" xlink:to="loc_ango_AcquisitionRestructuringAndOtherItemsNet_dab627b7-12fc-4f03-bd3f-15bd67fc077a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsForPreviousAcquisition_ac86f266-e8ec-415a-a32a-378ec6305eb2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsForPreviousAcquisition"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_c77246b2-da65-48be-99cb-b82293cc22b4" xlink:to="loc_us-gaap_PaymentsForPreviousAcquisition_ac86f266-e8ec-415a-a32a-378ec6305eb2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_aff00459-68ce-4d92-8fb1-17f7b7d893cd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfRestructuringAndRelatedCostsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_c77246b2-da65-48be-99cb-b82293cc22b4" xlink:to="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_aff00459-68ce-4d92-8fb1-17f7b7d893cd" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_6fbbdd2b-8fdc-4e54-8307-1e1240acb855" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_aff00459-68ce-4d92-8fb1-17f7b7d893cd" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_6fbbdd2b-8fdc-4e54-8307-1e1240acb855" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_6fbbdd2b-8fdc-4e54-8307-1e1240acb855_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_6fbbdd2b-8fdc-4e54-8307-1e1240acb855" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_6fbbdd2b-8fdc-4e54-8307-1e1240acb855_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_bea4cde1-9c1c-4018-bbec-7799596078c6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_6fbbdd2b-8fdc-4e54-8307-1e1240acb855" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_bea4cde1-9c1c-4018-bbec-7799596078c6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_LegalCostsMember_0f51ccd9-7283-4dbd-b4dc-5c1962e50f0c" xlink:href="ango-20230228.xsd#ango_LegalCostsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_bea4cde1-9c1c-4018-bbec-7799596078c6" xlink:to="loc_ango_LegalCostsMember_0f51ccd9-7283-4dbd-b4dc-5c1962e50f0c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_MergersandAcquisitionsMember_ef90229d-f828-4452-84be-16bf2256be29" xlink:href="ango-20230228.xsd#ango_MergersandAcquisitionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_bea4cde1-9c1c-4018-bbec-7799596078c6" xlink:to="loc_ango_MergersandAcquisitionsMember_ef90229d-f828-4452-84be-16bf2256be29" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_ManufacturingRelocationMember_41a8729b-9dec-4bb9-af53-c1dd2de80db3" xlink:href="ango-20230228.xsd#ango_ManufacturingRelocationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_bea4cde1-9c1c-4018-bbec-7799596078c6" xlink:to="loc_ango_ManufacturingRelocationMember_41a8729b-9dec-4bb9-af53-c1dd2de80db3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_IsraeliInnovationAuthorityPrepaymentMember_3bcd7d87-20e3-4739-8e00-399a14b5ddd7" xlink:href="ango-20230228.xsd#ango_IsraeliInnovationAuthorityPrepaymentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_bea4cde1-9c1c-4018-bbec-7799596078c6" xlink:to="loc_ango_IsraeliInnovationAuthorityPrepaymentMember_3bcd7d87-20e3-4739-8e00-399a14b5ddd7" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherExpenseMember_cea3dd40-2785-4b79-86ea-7ad05ffec383" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_bea4cde1-9c1c-4018-bbec-7799596078c6" xlink:to="loc_us-gaap_OtherExpenseMember_cea3dd40-2785-4b79-86ea-7ad05ffec383" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AccumulatedOtherComprehensiveIncomeDetails" xlink:type="simple" xlink:href="ango-20230228.xsd#AccumulatedOtherComprehensiveIncomeDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AccumulatedOtherComprehensiveIncomeDetails" xlink:type="extended" id="i29ceda1a0a8940a7a0ce814ecd018503_AccumulatedOtherComprehensiveIncomeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_6db3896a-b635-4ffe-a4bb-3fe99a7badeb" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_96fb21ff-e4b3-42e7-bffb-f85897f94aef" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AOCIAttributableToParentNetOfTaxRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_6db3896a-b635-4ffe-a4bb-3fe99a7badeb" xlink:to="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_96fb21ff-e4b3-42e7-bffb-f85897f94aef" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_453d456b-1782-4a7f-afd8-59eefbb89e67" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_96fb21ff-e4b3-42e7-bffb-f85897f94aef" xlink:to="loc_us-gaap_StockholdersEquity_453d456b-1782-4a7f-afd8-59eefbb89e67" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OciBeforeReclassificationsNetOfTaxAttributableToParent_cb0d6cce-6d23-44b7-b586-c6635d28c951" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OciBeforeReclassificationsNetOfTaxAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_96fb21ff-e4b3-42e7-bffb-f85897f94aef" xlink:to="loc_us-gaap_OciBeforeReclassificationsNetOfTaxAttributableToParent_cb0d6cce-6d23-44b7-b586-c6635d28c951" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_b61ad921-48e0-44a6-8876-2f3589f0130f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_96fb21ff-e4b3-42e7-bffb-f85897f94aef" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_b61ad921-48e0-44a6-8876-2f3589f0130f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_7cb0930e-6d7f-4593-881b-ca6c7f16a6a1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_95a30711-9b20-49ad-8334-71b28bed4877" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_6db3896a-b635-4ffe-a4bb-3fe99a7badeb" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_95a30711-9b20-49ad-8334-71b28bed4877" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_c0d93b12-8fd4-442f-bbe6-0b63b340e48c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_95a30711-9b20-49ad-8334-71b28bed4877" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_c0d93b12-8fd4-442f-bbe6-0b63b340e48c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_c0d93b12-8fd4-442f-bbe6-0b63b340e48c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_c0d93b12-8fd4-442f-bbe6-0b63b340e48c" xlink:to="loc_us-gaap_EquityComponentDomain_c0d93b12-8fd4-442f-bbe6-0b63b340e48c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_b6324409-fd04-4dd9-a290-d7d4b6a90921" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_c0d93b12-8fd4-442f-bbe6-0b63b340e48c" xlink:to="loc_us-gaap_EquityComponentDomain_b6324409-fd04-4dd9-a290-d7d4b6a90921" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedTranslationAdjustmentMember_b36a8aa8-658b-48cf-815b-f0c95a55b3a3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccumulatedTranslationAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_b6324409-fd04-4dd9-a290-d7d4b6a90921" xlink:to="loc_us-gaap_AccumulatedTranslationAdjustmentMember_b36a8aa8-658b-48cf-815b-f0c95a55b3a3" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://fasb.org/us-gaap/role/eedm/ExtensibleEnumerationLists" xlink:type="simple" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-roles-2021-01-31.xsd#eedm"/>
  <link:definitionLink xlink:role="http://fasb.org/us-gaap/role/eedm/ExtensibleEnumerationLists" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AssetsAbstract"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract" xlink:to="loc_us-gaap_OtherAssetsNoncurrent" xlink:type="arc" order="1"/>
  </link:definitionLink>
</link:linkbase>
