<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with Wdesk from Workiva-->
<!--Copyright 2020 Workiva-->
<!--r:2dbe5fae-ff01-439b-86f8-f5ea0445a4e1,g:f3c868df-d4c1-48b1-a673-e4fe0af3d3f6-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/CoverPage" xlink:type="simple" xlink:href="ango-20200831.xsd#CoverPage"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/CoverPage" xlink:type="extended" id="id952c841a89e467e8caafd6370c60676_CoverPage">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_d01e778b-d5bc-4086-954d-dfc22c33313f" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_AmendmentFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_AmendmentFlag_d01e778b-d5bc-4086-954d-dfc22c33313f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalYearFocus_21acc648-b5cd-44f2-bd55-84a37a469829" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentFiscalYearFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_DocumentFiscalYearFocus_21acc648-b5cd-44f2-bd55-84a37a469829" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalPeriodFocus_6920edc3-d402-4065-8cc3-d4fecb22560b" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentFiscalPeriodFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_DocumentFiscalPeriodFocus_6920edc3-d402-4065-8cc3-d4fecb22560b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_eecc6ace-9761-4576-a789-d0a6e4c8be96" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityCentralIndexKey"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_EntityCentralIndexKey_eecc6ace-9761-4576-a789-d0a6e4c8be96" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CurrentFiscalYearEndDate_410fe0e2-a2f8-40ff-80e8-83078c4e5ee9" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_CurrentFiscalYearEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_CurrentFiscalYearEndDate_410fe0e2-a2f8-40ff-80e8-83078c4e5ee9" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_NoTradingSymbolFlag_24f96b19-3ceb-43a1-9849-420ff486d7da" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_NoTradingSymbolFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_NoTradingSymbolFlag_24f96b19-3ceb-43a1-9849-420ff486d7da" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_068461ec-0587-4469-818a-50bde96ebdd5" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentType"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_DocumentType_068461ec-0587-4469-818a-50bde96ebdd5" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentQuarterlyReport_e022f91b-3818-472c-9ec2-b1581564698f" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentQuarterlyReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_DocumentQuarterlyReport_e022f91b-3818-472c-9ec2-b1581564698f" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentPeriodEndDate_3063f0ac-0174-455b-80ce-b2ea4f0a95d9" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentPeriodEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_DocumentPeriodEndDate_3063f0ac-0174-455b-80ce-b2ea4f0a95d9" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentTransitionReport_c8b6f56b-25d3-40b9-bfba-3c43505e5858" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_DocumentTransitionReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_DocumentTransitionReport_c8b6f56b-25d3-40b9-bfba-3c43505e5858" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_cdc956db-d60c-42f1-ab50-c17ddbca4b0b" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityFileNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_EntityFileNumber_cdc956db-d60c-42f1-ab50-c17ddbca4b0b" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_7ad46499-64ff-49db-8a0c-1bde7f78d5f8" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityRegistrantName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_EntityRegistrantName_7ad46499-64ff-49db-8a0c-1bde7f78d5f8" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_6034c146-4a0e-4ad9-ac7b-3dca8305a4b4" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_EntityIncorporationStateCountryCode_6034c146-4a0e-4ad9-ac7b-3dca8305a4b4" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_b45ffb3b-cbfb-4711-bcbe-60cc54896ec3" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_EntityTaxIdentificationNumber_b45ffb3b-cbfb-4711-bcbe-60cc54896ec3" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_e0b7c4ff-4140-4a81-8ee6-fbf57f69a826" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityAddressAddressLine1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_EntityAddressAddressLine1_e0b7c4ff-4140-4a81-8ee6-fbf57f69a826" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_5d017be3-e748-4285-b050-a03862e0240c" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityAddressCityOrTown"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_EntityAddressCityOrTown_5d017be3-e748-4285-b050-a03862e0240c" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressStateOrProvince_66dc3c46-ebeb-4281-80ac-9c4cdaeb1215" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityAddressStateOrProvince"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_EntityAddressStateOrProvince_66dc3c46-ebeb-4281-80ac-9c4cdaeb1215" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_01f11eae-057a-4fc7-9af4-c2731111a941" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityAddressPostalZipCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_EntityAddressPostalZipCode_01f11eae-057a-4fc7-9af4-c2731111a941" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_32507f9e-fc75-4d30-baca-991afe5c3bab" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_CityAreaCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_CityAreaCode_32507f9e-fc75-4d30-baca-991afe5c3bab" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_2ee4e756-85df-4fb3-a058-1499f2ccbea6" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_LocalPhoneNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_LocalPhoneNumber_2ee4e756-85df-4fb3-a058-1499f2ccbea6" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_78b58d79-3082-4d04-aad2-6f21c8eb7c71" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_Security12bTitle"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_Security12bTitle_78b58d79-3082-4d04-aad2-6f21c8eb7c71" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_923e726a-274e-4b3c-8877-699d1027ced2" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_TradingSymbol"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_TradingSymbol_923e726a-274e-4b3c-8877-699d1027ced2" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_1739524e-c2bc-442c-925e-21c7a0397fd1" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_SecurityExchangeName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_SecurityExchangeName_1739524e-c2bc-442c-925e-21c7a0397fd1" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCurrentReportingStatus_e3ca45ed-28ce-45d0-836b-a5b6e7e14e19" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityCurrentReportingStatus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_EntityCurrentReportingStatus_e3ca45ed-28ce-45d0-836b-a5b6e7e14e19" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityInteractiveDataCurrent_0979add1-76c2-4331-98a7-a29c2527e357" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityInteractiveDataCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_EntityInteractiveDataCurrent_0979add1-76c2-4331-98a7-a29c2527e357" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFilerCategory_6e364ca3-fd76-4987-83bc-9df9f0bb4d79" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityFilerCategory"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_EntityFilerCategory_6e364ca3-fd76-4987-83bc-9df9f0bb4d79" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntitySmallBusiness_a0bfb7c3-0939-4f16-86ff-1c9f8738c370" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntitySmallBusiness"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_EntitySmallBusiness_a0bfb7c3-0939-4f16-86ff-1c9f8738c370" xlink:type="arc" order="26"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_5badb802-c312-4ac0-acb1-c4920251a723" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_EntityEmergingGrowthCompany_5badb802-c312-4ac0-acb1-c4920251a723" xlink:type="arc" order="27"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityShellCompany_2428e07e-afe8-4ed1-ab64-6b9f50fa9478" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityShellCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_EntityShellCompany_2428e07e-afe8-4ed1-ab64-6b9f50fa9478" xlink:type="arc" order="28"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCommonStockSharesOutstanding_3abe3e86-2199-4ff0-bfed-20c1634791c5" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityCommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_dei_EntityCommonStockSharesOutstanding_3abe3e86-2199-4ff0-bfed-20c1634791c5" xlink:type="arc" order="29"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_f4afde04-9e52-48cf-8db3-0edcc5ae56cd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_cda27fad-27ac-413e-a68e-e2fe25a0f6bf" xlink:to="loc_us-gaap_StatementTable_f4afde04-9e52-48cf-8db3-0edcc5ae56cd" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_e61e3297-98ff-4632-ae9a-1fcab9453ab9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_f4afde04-9e52-48cf-8db3-0edcc5ae56cd" xlink:to="loc_us-gaap_StatementClassOfStockAxis_e61e3297-98ff-4632-ae9a-1fcab9453ab9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_e61e3297-98ff-4632-ae9a-1fcab9453ab9_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_e61e3297-98ff-4632-ae9a-1fcab9453ab9" xlink:to="loc_us-gaap_ClassOfStockDomain_e61e3297-98ff-4632-ae9a-1fcab9453ab9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_a56cecc6-ddd4-48cc-becb-d3e109c6ec94" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_e61e3297-98ff-4632-ae9a-1fcab9453ab9" xlink:to="loc_us-gaap_ClassOfStockDomain_a56cecc6-ddd4-48cc-becb-d3e109c6ec94" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_fa3f82c1-e0cb-4a1b-855d-0908f1bb9f72" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_a56cecc6-ddd4-48cc-becb-d3e109c6ec94" xlink:to="loc_us-gaap_CommonStockMember_fa3f82c1-e0cb-4a1b-855d-0908f1bb9f72" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_PreferredStockPurchaseRightsMember_bfe58541-1a91-49dd-be6f-05fb7c7e2a6d" xlink:href="ango-20200831.xsd#ango_PreferredStockPurchaseRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_a56cecc6-ddd4-48cc-becb-d3e109c6ec94" xlink:to="loc_ango_PreferredStockPurchaseRightsMember_bfe58541-1a91-49dd-be6f-05fb7c7e2a6d" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/ConsolidatedStatementsofOperations" xlink:type="simple" xlink:href="ango-20200831.xsd#ConsolidatedStatementsofOperations"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/ConsolidatedStatementsofOperations" xlink:type="extended" id="i0eb073572a66486ab9d3ef41a9f4cf3c_ConsolidatedStatementsofOperations"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/ConsolidatedStatementsofComprehensiveIncomeLoss" xlink:type="simple" xlink:href="ango-20200831.xsd#ConsolidatedStatementsofComprehensiveIncomeLoss"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/ConsolidatedStatementsofComprehensiveIncomeLoss" xlink:type="extended" id="i57bda5319bb54ee2b880ddcd57bb4396_ConsolidatedStatementsofComprehensiveIncomeLoss"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/ConsolidatedBalanceSheets" xlink:type="simple" xlink:href="ango-20200831.xsd#ConsolidatedBalanceSheets"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/ConsolidatedBalanceSheets" xlink:type="extended" id="ic32b6926a76c4238b0a52047a391b03e_ConsolidatedBalanceSheets"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/ConsolidatedBalanceSheetsParenthetical" xlink:type="simple" xlink:href="ango-20200831.xsd#ConsolidatedBalanceSheetsParenthetical"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/ConsolidatedBalanceSheetsParenthetical" xlink:type="extended" id="i913fc11068e546c6af9b06d39cec4643_ConsolidatedBalanceSheetsParenthetical"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/ConsolidatedStatementsofCashFlows" xlink:type="simple" xlink:href="ango-20200831.xsd#ConsolidatedStatementsofCashFlows"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/ConsolidatedStatementsofCashFlows" xlink:type="extended" id="ifc80e5662d53469b9e7a35ff21eebac2_ConsolidatedStatementsofCashFlows"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/ConsolidatedStatementsofStockholdersEquity" xlink:type="simple" xlink:href="ango-20200831.xsd#ConsolidatedStatementsofStockholdersEquity"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/ConsolidatedStatementsofStockholdersEquity" xlink:type="extended" id="i4292e2bdaf6849db92dfe46c19449b89_ConsolidatedStatementsofStockholdersEquity">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_b48b92da-e0c0-4aad-804f-d7a82358e20e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesIssued_5f814582-a821-4081-9bd2-039322df626e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b48b92da-e0c0-4aad-804f-d7a82358e20e" xlink:to="loc_us-gaap_SharesIssued_5f814582-a821-4081-9bd2-039322df626e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_4e6b4e85-0165-4a32-8012-18e666f2f86b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b48b92da-e0c0-4aad-804f-d7a82358e20e" xlink:to="loc_us-gaap_StockholdersEquity_4e6b4e85-0165-4a32-8012-18e666f2f86b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockShares_02fd2a0b-094a-486e-9e17-d617d474fb68" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TreasuryStockShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b48b92da-e0c0-4aad-804f-d7a82358e20e" xlink:to="loc_us-gaap_TreasuryStockShares_02fd2a0b-094a-486e-9e17-d617d474fb68" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_c8aab778-3338-4889-9e0f-f79a4bf0d41f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b48b92da-e0c0-4aad-804f-d7a82358e20e" xlink:to="loc_us-gaap_NetIncomeLoss_c8aab778-3338-4889-9e0f-f79a4bf0d41f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_7b9b1732-afce-4a57-9636-d00a4bf15cf9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b48b92da-e0c0-4aad-804f-d7a82358e20e" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_7b9b1732-afce-4a57-9636-d00a4bf15cf9" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_b14889dc-6a0a-4942-9be7-3450600af660" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b48b92da-e0c0-4aad-804f-d7a82358e20e" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_b14889dc-6a0a-4942-9be7-3450600af660" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation_b1461c8d-49f5-4b83-9b6d-350df6aec1f3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b48b92da-e0c0-4aad-804f-d7a82358e20e" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation_b1461c8d-49f5-4b83-9b6d-350df6aec1f3" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation_c6e6e33f-6e0d-4159-95cd-1a106309c116" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b48b92da-e0c0-4aad-804f-d7a82358e20e" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation_c6e6e33f-6e0d-4159-95cd-1a106309c116" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans_fd256e11-b8a2-4e3d-b122-43f22932c6bb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b48b92da-e0c0-4aad-804f-d7a82358e20e" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans_fd256e11-b8a2-4e3d-b122-43f22932c6bb" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan_f0214e10-8931-40e6-9513-30ab9e771439" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b48b92da-e0c0-4aad-804f-d7a82358e20e" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan_f0214e10-8931-40e6-9513-30ab9e771439" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_5d76770c-ccbb-4fc3-ae50-6f02beeb577f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b48b92da-e0c0-4aad-804f-d7a82358e20e" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_5d76770c-ccbb-4fc3-ae50-6f02beeb577f" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_39ec9b9c-ab1d-45cc-99ba-709e9bce45b8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b48b92da-e0c0-4aad-804f-d7a82358e20e" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_39ec9b9c-ab1d-45cc-99ba-709e9bce45b8" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesIssued_06908865-2540-4be9-a8b1-b498a0b77cef" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharesIssued"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_db209ef0-8482-477d-b456-daf41d0ab002" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockShares_ed6de557-db02-4724-990a-f19dcc487e35" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TreasuryStockShares"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_d3a0989f-1814-4396-a6fa-6a2640fd7b12" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_b48b92da-e0c0-4aad-804f-d7a82358e20e" xlink:to="loc_us-gaap_StatementTable_d3a0989f-1814-4396-a6fa-6a2640fd7b12" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_1675392a-15cc-41c4-9e43-e4b382df795d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_d3a0989f-1814-4396-a6fa-6a2640fd7b12" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_1675392a-15cc-41c4-9e43-e4b382df795d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_1675392a-15cc-41c4-9e43-e4b382df795d_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_1675392a-15cc-41c4-9e43-e4b382df795d" xlink:to="loc_us-gaap_EquityComponentDomain_1675392a-15cc-41c4-9e43-e4b382df795d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_d8d080c1-f367-4eb9-8c45-c1c34b3e6c1e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_1675392a-15cc-41c4-9e43-e4b382df795d" xlink:to="loc_us-gaap_EquityComponentDomain_d8d080c1-f367-4eb9-8c45-c1c34b3e6c1e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_63ecc210-9541-4de9-a223-ddbd1eeba4ec" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_d8d080c1-f367-4eb9-8c45-c1c34b3e6c1e" xlink:to="loc_us-gaap_CommonStockMember_63ecc210-9541-4de9-a223-ddbd1eeba4ec" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_d1085ceb-2523-445d-aee3-e47d1ca417c2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_d8d080c1-f367-4eb9-8c45-c1c34b3e6c1e" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_d1085ceb-2523-445d-aee3-e47d1ca417c2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_4d54c1e1-094f-454c-84e5-500bc3d3c93d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_d8d080c1-f367-4eb9-8c45-c1c34b3e6c1e" xlink:to="loc_us-gaap_RetainedEarningsMember_4d54c1e1-094f-454c-84e5-500bc3d3c93d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_26e58867-caa3-43af-8761-10e745b6712b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_d8d080c1-f367-4eb9-8c45-c1c34b3e6c1e" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_26e58867-caa3-43af-8761-10e745b6712b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockMember_5a55a078-4754-469e-b590-22701ea06a18" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TreasuryStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_d8d080c1-f367-4eb9-8c45-c1c34b3e6c1e" xlink:to="loc_us-gaap_TreasuryStockMember_5a55a078-4754-469e-b590-22701ea06a18" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_afc177b4-eb20-4193-8332-151fc20570eb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_d3a0989f-1814-4396-a6fa-6a2640fd7b12" xlink:to="loc_us-gaap_AwardTypeAxis_afc177b4-eb20-4193-8332-151fc20570eb" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_afc177b4-eb20-4193-8332-151fc20570eb_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_afc177b4-eb20-4193-8332-151fc20570eb" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_afc177b4-eb20-4193-8332-151fc20570eb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_55ecce23-334c-4738-a952-6832fd5646e8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_afc177b4-eb20-4193-8332-151fc20570eb" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_55ecce23-334c-4738-a952-6832fd5646e8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_aa618cfd-28f4-451e-867e-14f651b2290a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_55ecce23-334c-4738-a952-6832fd5646e8" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_aa618cfd-28f4-451e-867e-14f651b2290a" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/ConsolidatedFinancialStatements" xlink:type="simple" xlink:href="ango-20200831.xsd#ConsolidatedFinancialStatements"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/ConsolidatedFinancialStatements" xlink:type="extended" id="i5ab479b12e0c4914901f10e686768a63_ConsolidatedFinancialStatements"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/Acquisitions" xlink:type="simple" xlink:href="ango-20200831.xsd#Acquisitions"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/Acquisitions" xlink:type="extended" id="i894c4164ad5d484e91076b0e995bf1a0_Acquisitions"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AcquisitionsTables" xlink:type="simple" xlink:href="ango-20200831.xsd#AcquisitionsTables"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AcquisitionsTables" xlink:type="extended" id="i6edd086162154497b908504fc0ab5b35_AcquisitionsTables"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AcquisitionsNarrativeDetails" xlink:type="simple" xlink:href="ango-20200831.xsd#AcquisitionsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AcquisitionsNarrativeDetails" xlink:type="extended" id="iccf16ba942ff4d5dac687abde39ac573_AcquisitionsNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionLineItems_77868860-1852-4aae-a118-0878e79c9508" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_AssetAcquisitionConsiderationTransferred_4e1d6bb0-0a88-4032-9b4e-de7437ead815" xlink:href="ango-20200831.xsd#ango_AssetAcquisitionConsiderationTransferred"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_77868860-1852-4aae-a118-0878e79c9508" xlink:to="loc_ango_AssetAcquisitionConsiderationTransferred_4e1d6bb0-0a88-4032-9b4e-de7437ead815" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_AssetAcquisitionContingentConsiderationLiability_4c4d2a2f-ae57-4b68-b47f-44858ac151b9" xlink:href="ango-20200831.xsd#ango_AssetAcquisitionContingentConsiderationLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_77868860-1852-4aae-a118-0878e79c9508" xlink:to="loc_ango_AssetAcquisitionContingentConsiderationLiability_4c4d2a2f-ae57-4b68-b47f-44858ac151b9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_AssetAcquisitionRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInventory_e00185cc-fca0-458e-aac8-920fe9ed02a2" xlink:href="ango-20200831.xsd#ango_AssetAcquisitionRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInventory"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_77868860-1852-4aae-a118-0878e79c9508" xlink:to="loc_ango_AssetAcquisitionRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInventory_e00185cc-fca0-458e-aac8-920fe9ed02a2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_AssetAcquisitionRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedFiniteLivedIntangibles_633662cc-727b-465f-9f47-6f995c6f863a" xlink:href="ango-20200831.xsd#ango_AssetAcquisitionRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedFiniteLivedIntangibles"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_77868860-1852-4aae-a118-0878e79c9508" xlink:to="loc_ango_AssetAcquisitionRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedFiniteLivedIntangibles_633662cc-727b-465f-9f47-6f995c6f863a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_5a7f7935-0774-4c52-911c-45de45adcbf8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_77868860-1852-4aae-a118-0878e79c9508" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_5a7f7935-0774-4c52-911c-45de45adcbf8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationConsiderationTransferred1_970b3e28-c219-4a6a-a56a-364ff69b7cbb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationConsiderationTransferred1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_77868860-1852-4aae-a118-0878e79c9508" xlink:to="loc_us-gaap_BusinessCombinationConsiderationTransferred1_970b3e28-c219-4a6a-a56a-364ff69b7cbb" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireBusinessesGross_d688d49f-0ff6-44dd-977c-4ff6b6640203" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentsToAcquireBusinessesGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_77868860-1852-4aae-a118-0878e79c9508" xlink:to="loc_us-gaap_PaymentsToAcquireBusinessesGross_d688d49f-0ff6-44dd-977c-4ff6b6640203" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_b1ed4cb4-8cca-49a9-bdd5-638ed29f634b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationContingentConsiderationLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_77868860-1852-4aae-a118-0878e79c9508" xlink:to="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_b1ed4cb4-8cca-49a9-bdd5-638ed29f634b" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationAcquisitionRelatedCosts_6439785f-c98d-4c86-aaf0-16b4a9abd744" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationAcquisitionRelatedCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_77868860-1852-4aae-a118-0878e79c9508" xlink:to="loc_us-gaap_BusinessCombinationAcquisitionRelatedCosts_6439785f-c98d-4c86-aaf0-16b4a9abd744" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_089b784e-424b-452d-a703-4569173bfda9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_77868860-1852-4aae-a118-0878e79c9508" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_089b784e-424b-452d-a703-4569173bfda9" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_470ed961-10a4-42d2-b092-93ff11cbbd0f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_089b784e-424b-452d-a703-4569173bfda9" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_470ed961-10a4-42d2-b092-93ff11cbbd0f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_470ed961-10a4-42d2-b092-93ff11cbbd0f_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_470ed961-10a4-42d2-b092-93ff11cbbd0f" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_470ed961-10a4-42d2-b092-93ff11cbbd0f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_3c764439-ee1c-424a-b68a-2c3062397b30" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_470ed961-10a4-42d2-b092-93ff11cbbd0f" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_3c764439-ee1c-424a-b68a-2c3062397b30" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_EximoMedicalLtd.Member_d5ba9496-766c-4bc2-bcdb-0004c008cc3d" xlink:href="ango-20200831.xsd#ango_EximoMedicalLtd.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_3c764439-ee1c-424a-b68a-2c3062397b30" xlink:to="loc_ango_EximoMedicalLtd.Member_d5ba9496-766c-4bc2-bcdb-0004c008cc3d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_27cc3b98-2f2d-4632-a657-9eaad1ee7f63" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_089b784e-424b-452d-a703-4569173bfda9" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_27cc3b98-2f2d-4632-a657-9eaad1ee7f63" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_27cc3b98-2f2d-4632-a657-9eaad1ee7f63_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_27cc3b98-2f2d-4632-a657-9eaad1ee7f63" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_27cc3b98-2f2d-4632-a657-9eaad1ee7f63_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_4ee047cc-4de2-46fb-8bd9-0407ac6e7925" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_27cc3b98-2f2d-4632-a657-9eaad1ee7f63" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_4ee047cc-4de2-46fb-8bd9-0407ac6e7925" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TrademarksMember_fb9b5846-71ff-4b8f-8471-4da082db2ce5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TrademarksMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_4ee047cc-4de2-46fb-8bd9-0407ac6e7925" xlink:to="loc_us-gaap_TrademarksMember_fb9b5846-71ff-4b8f-8471-4da082db2ce5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TechnologyBasedIntangibleAssetsMember_85abda69-3a2d-4d60-8c3b-17d14f01ce88" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TechnologyBasedIntangibleAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_4ee047cc-4de2-46fb-8bd9-0407ac6e7925" xlink:to="loc_us-gaap_TechnologyBasedIntangibleAssetsMember_85abda69-3a2d-4d60-8c3b-17d14f01ce88" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_AssetAcquisitionAxis_dc0ff873-dd5c-48f3-ac1d-3b3dad349ab2" xlink:href="ango-20200831.xsd#ango_AssetAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_089b784e-424b-452d-a703-4569173bfda9" xlink:to="loc_ango_AssetAcquisitionAxis_dc0ff873-dd5c-48f3-ac1d-3b3dad349ab2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_AssetAcquisitionDomain_dc0ff873-dd5c-48f3-ac1d-3b3dad349ab2_default" xlink:href="ango-20200831.xsd#ango_AssetAcquisitionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ango_AssetAcquisitionAxis_dc0ff873-dd5c-48f3-ac1d-3b3dad349ab2" xlink:to="loc_ango_AssetAcquisitionDomain_dc0ff873-dd5c-48f3-ac1d-3b3dad349ab2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_AssetAcquisitionDomain_7a2aa412-27a9-44b9-ac0e-70ceaf8c01bf" xlink:href="ango-20200831.xsd#ango_AssetAcquisitionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ango_AssetAcquisitionAxis_dc0ff873-dd5c-48f3-ac1d-3b3dad349ab2" xlink:to="loc_ango_AssetAcquisitionDomain_7a2aa412-27a9-44b9-ac0e-70ceaf8c01bf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_C3WaveTipLocationAssetMember_40b5fbaf-c01a-4b83-87ef-1c15b3ecd013" xlink:href="ango-20200831.xsd#ango_C3WaveTipLocationAssetMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ango_AssetAcquisitionDomain_7a2aa412-27a9-44b9-ac0e-70ceaf8c01bf" xlink:to="loc_ango_C3WaveTipLocationAssetMember_40b5fbaf-c01a-4b83-87ef-1c15b3ecd013" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AcquisitionsDetails" xlink:type="simple" xlink:href="ango-20200831.xsd#AcquisitionsDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AcquisitionsDetails" xlink:type="extended" id="ie892b3a7161140a2ac378e7867c91f12_AcquisitionsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionLineItems_595af72a-20bb-459f-9982-b49b86b70021" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_954188b1-6eec-4c99-ae36-98d3772c8c7b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_595af72a-20bb-459f-9982-b49b86b70021" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_954188b1-6eec-4c99-ae36-98d3772c8c7b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsReceivables_763e2fff-7945-4a77-aceb-29b2784d6f3a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsReceivables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_954188b1-6eec-4c99-ae36-98d3772c8c7b" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsReceivables_763e2fff-7945-4a77-aceb-29b2784d6f3a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInventory_3b20fafc-c10a-4fc5-a3d8-fff3523929ff" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInventory"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_954188b1-6eec-4c99-ae36-98d3772c8c7b" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInventory_3b20fafc-c10a-4fc5-a3d8-fff3523929ff" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsPrepaidExpenseAndOtherAssets_e454db1a-bd5a-479f-b127-352b4b4af12d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsPrepaidExpenseAndOtherAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_954188b1-6eec-4c99-ae36-98d3772c8c7b" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsPrepaidExpenseAndOtherAssets_e454db1a-bd5a-479f-b127-352b4b4af12d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLongTermDeposits_7ce0a561-6fb7-4c8a-9c8e-5ebcc939696f" xlink:href="ango-20200831.xsd#ango_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLongTermDeposits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_954188b1-6eec-4c99-ae36-98d3772c8c7b" xlink:to="loc_ango_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLongTermDeposits_7ce0a561-6fb7-4c8a-9c8e-5ebcc939696f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedPropertyPlantAndEquipment_dd9a72c6-86ba-473b-a506-70401180d37e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_954188b1-6eec-4c99-ae36-98d3772c8c7b" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedPropertyPlantAndEquipment_dd9a72c6-86ba-473b-a506-70401180d37e" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibles_7b2f89ff-f788-4b5f-b9e1-1712bc1a216b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibles"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_954188b1-6eec-4c99-ae36-98d3772c8c7b" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibles_7b2f89ff-f788-4b5f-b9e1-1712bc1a216b" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_f05279c5-0b37-42b7-8283-65eb837435f6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_954188b1-6eec-4c99-ae36-98d3772c8c7b" xlink:to="loc_us-gaap_Goodwill_f05279c5-0b37-42b7-8283-65eb837435f6" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsIncludingGoodwill_228af597-0f46-4776-aba8-30e153f2e489" xlink:href="ango-20200831.xsd#ango_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsIncludingGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_954188b1-6eec-4c99-ae36-98d3772c8c7b" xlink:to="loc_ango_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsIncludingGoodwill_228af597-0f46-4776-aba8-30e153f2e489" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoncashOrPartNoncashAcquisitionValueOfLiabilitiesAssumedAbstract_78413bcd-f354-4c5a-a434-6eebc7215b00" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NoncashOrPartNoncashAcquisitionValueOfLiabilitiesAssumedAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_595af72a-20bb-459f-9982-b49b86b70021" xlink:to="loc_us-gaap_NoncashOrPartNoncashAcquisitionValueOfLiabilitiesAssumedAbstract_78413bcd-f354-4c5a-a434-6eebc7215b00" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesAccountsPayable_56a67346-2dc3-44fe-aeef-c62470f9644f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesAccountsPayable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoncashOrPartNoncashAcquisitionValueOfLiabilitiesAssumedAbstract_78413bcd-f354-4c5a-a434-6eebc7215b00" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesAccountsPayable_56a67346-2dc3-44fe-aeef-c62470f9644f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesOther_f44b8c3e-38de-495e-b0b1-84777ebf88c5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesOther"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoncashOrPartNoncashAcquisitionValueOfLiabilitiesAssumedAbstract_78413bcd-f354-4c5a-a434-6eebc7215b00" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesOther_f44b8c3e-38de-495e-b0b1-84777ebf88c5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedDeferredTaxLiabilities_7dbe4c20-f410-4e2a-b578-48ebbfd7a07f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedDeferredTaxLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoncashOrPartNoncashAcquisitionValueOfLiabilitiesAssumedAbstract_78413bcd-f354-4c5a-a434-6eebc7215b00" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedDeferredTaxLiabilities_7dbe4c20-f410-4e2a-b578-48ebbfd7a07f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilities_29ab269e-bd5e-4fa0-984f-296f0826b715" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoncashOrPartNoncashAcquisitionValueOfLiabilitiesAssumedAbstract_78413bcd-f354-4c5a-a434-6eebc7215b00" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilities_29ab269e-bd5e-4fa0-984f-296f0826b715" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredGoodwillAndLiabilitiesAssumedNet_6bf975c1-29b0-4982-bf8b-3b6b52567704" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredGoodwillAndLiabilitiesAssumedNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_595af72a-20bb-459f-9982-b49b86b70021" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredGoodwillAndLiabilitiesAssumedNet_6bf975c1-29b0-4982-bf8b-3b6b52567704" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_780346a5-98f6-4de0-862f-3d4b9d016c47" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_595af72a-20bb-459f-9982-b49b86b70021" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_780346a5-98f6-4de0-862f-3d4b9d016c47" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_787c9559-028f-4e5d-b8e8-4553b1321525" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_780346a5-98f6-4de0-862f-3d4b9d016c47" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_787c9559-028f-4e5d-b8e8-4553b1321525" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_787c9559-028f-4e5d-b8e8-4553b1321525_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_787c9559-028f-4e5d-b8e8-4553b1321525" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_787c9559-028f-4e5d-b8e8-4553b1321525_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_b975a4af-3db5-498b-87a4-32479c3e8048" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_787c9559-028f-4e5d-b8e8-4553b1321525" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_b975a4af-3db5-498b-87a4-32479c3e8048" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_EximoMedicalLtd.Member_f00f68ae-4c75-48f2-8350-d0e0d5e03e77" xlink:href="ango-20200831.xsd#ango_EximoMedicalLtd.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_b975a4af-3db5-498b-87a4-32479c3e8048" xlink:to="loc_ango_EximoMedicalLtd.Member_f00f68ae-4c75-48f2-8350-d0e0d5e03e77" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_89bf605b-fce8-4f5a-94a1-ed35cf8c3397" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_780346a5-98f6-4de0-862f-3d4b9d016c47" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_89bf605b-fce8-4f5a-94a1-ed35cf8c3397" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_89bf605b-fce8-4f5a-94a1-ed35cf8c3397_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_89bf605b-fce8-4f5a-94a1-ed35cf8c3397" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_89bf605b-fce8-4f5a-94a1-ed35cf8c3397_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_3521da65-ae34-4146-a329-0cca6cccfe17" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_89bf605b-fce8-4f5a-94a1-ed35cf8c3397" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_3521da65-ae34-4146-a329-0cca6cccfe17" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TechnologyBasedIntangibleAssetsMember_e220dad6-6467-4020-86ea-5fcbb2061aa4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TechnologyBasedIntangibleAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_3521da65-ae34-4146-a329-0cca6cccfe17" xlink:to="loc_us-gaap_TechnologyBasedIntangibleAssetsMember_e220dad6-6467-4020-86ea-5fcbb2061aa4" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/RevenuefromContractswithCustomers" xlink:type="simple" xlink:href="ango-20200831.xsd#RevenuefromContractswithCustomers"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/RevenuefromContractswithCustomers" xlink:type="extended" id="idcbcc426e0454aefb06ade13938c5c27_RevenuefromContractswithCustomers"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/RevenuefromContractswithCustomersTables" xlink:type="simple" xlink:href="ango-20200831.xsd#RevenuefromContractswithCustomersTables"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/RevenuefromContractswithCustomersTables" xlink:type="extended" id="i40aa56241c234e69882c91315cb848fb_RevenuefromContractswithCustomersTables"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/RevenuefromContractswithCustomersDisaggregationofRevenueDetails" xlink:type="simple" xlink:href="ango-20200831.xsd#RevenuefromContractswithCustomersDisaggregationofRevenueDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/RevenuefromContractswithCustomersDisaggregationofRevenueDetails" xlink:type="extended" id="ia6acd0ae19a34e97807f66b48c2c5fd8_RevenuefromContractswithCustomersDisaggregationofRevenueDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_f485b600-9be7-4b07-ab48-cfad1098026b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Revenues_36427e00-fd82-4c9b-b91b-81509fc61b18" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_Revenues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_f485b600-9be7-4b07-ab48-cfad1098026b" xlink:to="loc_us-gaap_Revenues_36427e00-fd82-4c9b-b91b-81509fc61b18" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueTable_b6f90006-83ab-49bf-bdaa-a1248429c49f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DisaggregationOfRevenueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_f485b600-9be7-4b07-ab48-cfad1098026b" xlink:to="loc_us-gaap_DisaggregationOfRevenueTable_b6f90006-83ab-49bf-bdaa-a1248429c49f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_eaea3b23-9222-4fa6-8131-ee6a3f2f9ce1" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_b6f90006-83ab-49bf-bdaa-a1248429c49f" xlink:to="loc_srt_ProductOrServiceAxis_eaea3b23-9222-4fa6-8131-ee6a3f2f9ce1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_eaea3b23-9222-4fa6-8131-ee6a3f2f9ce1_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_eaea3b23-9222-4fa6-8131-ee6a3f2f9ce1" xlink:to="loc_srt_ProductsAndServicesDomain_eaea3b23-9222-4fa6-8131-ee6a3f2f9ce1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_a4e36d20-a682-4018-bc5d-7f2df5d45a3d" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_eaea3b23-9222-4fa6-8131-ee6a3f2f9ce1" xlink:to="loc_srt_ProductsAndServicesDomain_a4e36d20-a682-4018-bc5d-7f2df5d45a3d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_VascularInterventionsandTherapiesMember_94093f19-6c1a-444d-8c0b-8f81e2f74ca7" xlink:href="ango-20200831.xsd#ango_VascularInterventionsandTherapiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_a4e36d20-a682-4018-bc5d-7f2df5d45a3d" xlink:to="loc_ango_VascularInterventionsandTherapiesMember_94093f19-6c1a-444d-8c0b-8f81e2f74ca7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_VascularAccessMember_a59df436-7930-4ee8-81a2-10be93cfb705" xlink:href="ango-20200831.xsd#ango_VascularAccessMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_a4e36d20-a682-4018-bc5d-7f2df5d45a3d" xlink:to="loc_ango_VascularAccessMember_a59df436-7930-4ee8-81a2-10be93cfb705" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_OncologyMember_111328d6-2abf-4e43-bbca-49bbb0ea2f7d" xlink:href="ango-20200831.xsd#ango_OncologyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_a4e36d20-a682-4018-bc5d-7f2df5d45a3d" xlink:to="loc_ango_OncologyMember_111328d6-2abf-4e43-bbca-49bbb0ea2f7d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_65db50e4-b0f2-4852-965e-80de01ab5ab2" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_b6f90006-83ab-49bf-bdaa-a1248429c49f" xlink:to="loc_srt_StatementGeographicalAxis_65db50e4-b0f2-4852-965e-80de01ab5ab2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_65db50e4-b0f2-4852-965e-80de01ab5ab2_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_65db50e4-b0f2-4852-965e-80de01ab5ab2" xlink:to="loc_srt_SegmentGeographicalDomain_65db50e4-b0f2-4852-965e-80de01ab5ab2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_af18aaa8-09bd-431d-9bcc-2d792d4fc792" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_65db50e4-b0f2-4852-965e-80de01ab5ab2" xlink:to="loc_srt_SegmentGeographicalDomain_af18aaa8-09bd-431d-9bcc-2d792d4fc792" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_e7612cda-87dc-4d20-8d5d-72325a74a56d" xlink:href="https://xbrl.sec.gov/country/2020/country-2020-01-31.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_af18aaa8-09bd-431d-9bcc-2d792d4fc792" xlink:to="loc_country_US_e7612cda-87dc-4d20-8d5d-72325a74a56d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonUsMember_2b144681-834c-4c84-b900-6429a7e59864" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NonUsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_af18aaa8-09bd-431d-9bcc-2d792d4fc792" xlink:to="loc_us-gaap_NonUsMember_2b144681-834c-4c84-b900-6429a7e59864" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/RevenuefromContractswithCustomersNarrativeDetails" xlink:type="simple" xlink:href="ango-20200831.xsd#RevenuefromContractswithCustomersNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/RevenuefromContractswithCustomersNarrativeDetails" xlink:type="extended" id="i63854d9a3e1a42048a5583651c78419f_RevenuefromContractswithCustomersNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_8c01f724-fcb6-4819-8c43-e87081bef5b5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_RevenuePerformanceObligationPaymentTerm_74e53f79-8fd6-4b22-bba9-f03de2bdb66e" xlink:href="ango-20200831.xsd#ango_RevenuePerformanceObligationPaymentTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_8c01f724-fcb6-4819-8c43-e87081bef5b5" xlink:to="loc_ango_RevenuePerformanceObligationPaymentTerm_74e53f79-8fd6-4b22-bba9-f03de2bdb66e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_ContractwithCustomerRightofReturnProductReturnPeriodAfterPurchaseDateRequiringPreApproval_ec79e842-f5d7-496f-966c-3bcbde59f958" xlink:href="ango-20200831.xsd#ango_ContractwithCustomerRightofReturnProductReturnPeriodAfterPurchaseDateRequiringPreApproval"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_8c01f724-fcb6-4819-8c43-e87081bef5b5" xlink:to="loc_ango_ContractwithCustomerRightofReturnProductReturnPeriodAfterPurchaseDateRequiringPreApproval_ec79e842-f5d7-496f-966c-3bcbde59f958" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_ContractwithCustomerRightofReturnRestockingCharge_0315e3d5-f3ae-4d11-8574-7c0a956f8756" xlink:href="ango-20200831.xsd#ango_ContractwithCustomerRightofReturnRestockingCharge"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_8c01f724-fcb6-4819-8c43-e87081bef5b5" xlink:to="loc_ango_ContractwithCustomerRightofReturnRestockingCharge_0315e3d5-f3ae-4d11-8574-7c0a956f8756" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_ContractwithCustomerRightofReturnMinimumRemainingProductLifePriortoReturn_b1891d55-43d1-4d3d-9842-411c03c0e07a" xlink:href="ango-20200831.xsd#ango_ContractwithCustomerRightofReturnMinimumRemainingProductLifePriortoReturn"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_8c01f724-fcb6-4819-8c43-e87081bef5b5" xlink:to="loc_ango_ContractwithCustomerRightofReturnMinimumRemainingProductLifePriortoReturn_b1891d55-43d1-4d3d-9842-411c03c0e07a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityIncreaseDecreaseForContractAcquiredInBusinessCombination_bb430776-dfe4-428c-b4a5-2387fd332ca0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContractWithCustomerLiabilityIncreaseDecreaseForContractAcquiredInBusinessCombination"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_8c01f724-fcb6-4819-8c43-e87081bef5b5" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityIncreaseDecreaseForContractAcquiredInBusinessCombination_bb430776-dfe4-428c-b4a5-2387fd332ca0" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityRevenueRecognized_99bd5e34-ac0c-405f-a062-edc3aa8e8612" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContractWithCustomerLiabilityRevenueRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_8c01f724-fcb6-4819-8c43-e87081bef5b5" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityRevenueRecognized_99bd5e34-ac0c-405f-a062-edc3aa8e8612" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueTable_dfb5e712-126c-465f-9492-f8913f325024" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DisaggregationOfRevenueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_8c01f724-fcb6-4819-8c43-e87081bef5b5" xlink:to="loc_us-gaap_DisaggregationOfRevenueTable_dfb5e712-126c-465f-9492-f8913f325024" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_2c5f8f5f-85fc-4b23-b35c-2d2ca8b02d02" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_dfb5e712-126c-465f-9492-f8913f325024" xlink:to="loc_srt_RangeAxis_2c5f8f5f-85fc-4b23-b35c-2d2ca8b02d02" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_2c5f8f5f-85fc-4b23-b35c-2d2ca8b02d02_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_2c5f8f5f-85fc-4b23-b35c-2d2ca8b02d02" xlink:to="loc_srt_RangeMember_2c5f8f5f-85fc-4b23-b35c-2d2ca8b02d02_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_ea1db03c-0e37-46ca-b4ea-71515efe85b0" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_2c5f8f5f-85fc-4b23-b35c-2d2ca8b02d02" xlink:to="loc_srt_RangeMember_ea1db03c-0e37-46ca-b4ea-71515efe85b0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_afcb8b4f-367d-4da9-82f3-f91bf4fc5f23" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_ea1db03c-0e37-46ca-b4ea-71515efe85b0" xlink:to="loc_srt_MinimumMember_afcb8b4f-367d-4da9-82f3-f91bf4fc5f23" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_1c24fb7f-9fe8-449e-b61b-8214acf3598f" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_ea1db03c-0e37-46ca-b4ea-71515efe85b0" xlink:to="loc_srt_MaximumMember_1c24fb7f-9fe8-449e-b61b-8214acf3598f" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/RevenuefromContractswithCustomersContractBalanceswithCustomersDetails" xlink:type="simple" xlink:href="ango-20200831.xsd#RevenuefromContractswithCustomersContractBalanceswithCustomersDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/RevenuefromContractswithCustomersContractBalanceswithCustomersDetails" xlink:type="extended" id="i5ddf745faa9c4d67ac197405c1815c45_RevenuefromContractswithCustomersContractBalanceswithCustomersDetails"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/Inventories" xlink:type="simple" xlink:href="ango-20200831.xsd#Inventories"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/Inventories" xlink:type="extended" id="i7797494a3e9d486f813e455c71d55a52_Inventories"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/InventoriesTables" xlink:type="simple" xlink:href="ango-20200831.xsd#InventoriesTables"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/InventoriesTables" xlink:type="extended" id="i076cb0e1adc64f509cac46f7070ff28d_InventoriesTables"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/InventoriesScheduleofInventoriesDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#InventoriesScheduleofInventoriesDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/InventoriesScheduleofInventoriesDetail" xlink:type="extended" id="if4f5d0e119514d3ab09e88c2db6384c9_InventoriesScheduleofInventoriesDetail"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/InventoriesNarrativeDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#InventoriesNarrativeDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/InventoriesNarrativeDetail" xlink:type="extended" id="ib30493f68d4346f88bd64d5d0b05c031_InventoriesNarrativeDetail"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/GoodwillandIntangibleAssets" xlink:type="simple" xlink:href="ango-20200831.xsd#GoodwillandIntangibleAssets"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/GoodwillandIntangibleAssets" xlink:type="extended" id="i26a3fd13a0f44f9eb5f527f12cd1c92e_GoodwillandIntangibleAssets"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/GoodwillandIntangibleAssetsTables" xlink:type="simple" xlink:href="ango-20200831.xsd#GoodwillandIntangibleAssetsTables"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/GoodwillandIntangibleAssetsTables" xlink:type="extended" id="i9cb08e46896a48b28269b4c9df06c125_GoodwillandIntangibleAssetsTables"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/GoodwillandIntangibleAssetsNarrativeDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#GoodwillandIntangibleAssetsNarrativeDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/GoodwillandIntangibleAssetsNarrativeDetail" xlink:type="extended" id="if15a84fca32448d39f57dd7baaf55656_GoodwillandIntangibleAssetsNarrativeDetail">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_532367b1-0c96-48ee-bbf8-1104f6ca718f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_883e4715-05ab-4664-85ca-a301aeddceaa" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_532367b1-0c96-48ee-bbf8-1104f6ca718f" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_883e4715-05ab-4664-85ca-a301aeddceaa" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfReportableSegments_3fdb6ff9-0f93-4a9e-8ddf-7ec5d28c0976" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NumberOfReportableSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_532367b1-0c96-48ee-bbf8-1104f6ca718f" xlink:to="loc_us-gaap_NumberOfReportableSegments_3fdb6ff9-0f93-4a9e-8ddf-7ec5d28c0976" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfIntangibleAssets_b1e70306-2049-4323-95f4-cd57619a7408" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AmortizationOfIntangibleAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_532367b1-0c96-48ee-bbf8-1104f6ca718f" xlink:to="loc_us-gaap_AmortizationOfIntangibleAssets_b1e70306-2049-4323-95f4-cd57619a7408" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_a003ff39-2609-42b3-9e4a-7146c7e242cd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_532367b1-0c96-48ee-bbf8-1104f6ca718f" xlink:to="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_a003ff39-2609-42b3-9e4a-7146c7e242cd" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_230a0a86-cee7-4690-ab57-e8518afc9508" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_a003ff39-2609-42b3-9e4a-7146c7e242cd" xlink:to="loc_srt_RangeAxis_230a0a86-cee7-4690-ab57-e8518afc9508" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_230a0a86-cee7-4690-ab57-e8518afc9508_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_230a0a86-cee7-4690-ab57-e8518afc9508" xlink:to="loc_srt_RangeMember_230a0a86-cee7-4690-ab57-e8518afc9508_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_b938cd2f-6578-4103-92c8-d37b92304f1e" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_230a0a86-cee7-4690-ab57-e8518afc9508" xlink:to="loc_srt_RangeMember_b938cd2f-6578-4103-92c8-d37b92304f1e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_4c1b7890-fa04-4012-b621-acbebd57dbfa" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_b938cd2f-6578-4103-92c8-d37b92304f1e" xlink:to="loc_srt_MinimumMember_4c1b7890-fa04-4012-b621-acbebd57dbfa" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_02f503f7-47f6-4d64-8984-2426453bab4f" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_b938cd2f-6578-4103-92c8-d37b92304f1e" xlink:to="loc_srt_MaximumMember_02f503f7-47f6-4d64-8984-2426453bab4f" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/GoodwillandIntangibleAssetsIntangibleAssetsDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#GoodwillandIntangibleAssetsIntangibleAssetsDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/GoodwillandIntangibleAssetsIntangibleAssetsDetail" xlink:type="extended" id="i1c607d6643fe475f9d7937e1e18bc374_GoodwillandIntangibleAssetsIntangibleAssetsDetail">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_28c6b01a-c97c-4245-8c70-4c855dabfd6a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_3e5cd2a7-f3dd-46ba-90aa-8cd169eebd68" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_28c6b01a-c97c-4245-8c70-4c855dabfd6a" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_3e5cd2a7-f3dd-46ba-90aa-8cd169eebd68" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_1e725a74-72a5-4709-bcae-5895e4748998" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_28c6b01a-c97c-4245-8c70-4c855dabfd6a" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_1e725a74-72a5-4709-bcae-5895e4748998" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_e555c1ec-d6bd-41b6-9ce4-6a006b6aab21" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_28c6b01a-c97c-4245-8c70-4c855dabfd6a" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNet_e555c1ec-d6bd-41b6-9ce4-6a006b6aab21" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_00153fb1-7df4-44b6-8bb6-263a52ebfd2c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_28c6b01a-c97c-4245-8c70-4c855dabfd6a" xlink:to="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_00153fb1-7df4-44b6-8bb6-263a52ebfd2c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_37c937c9-5b17-47f7-a4dc-f806c6150984" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_00153fb1-7df4-44b6-8bb6-263a52ebfd2c" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_37c937c9-5b17-47f7-a4dc-f806c6150984" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_37c937c9-5b17-47f7-a4dc-f806c6150984_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_37c937c9-5b17-47f7-a4dc-f806c6150984" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_37c937c9-5b17-47f7-a4dc-f806c6150984_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_deec79e9-670f-4c0b-93c8-9c1fd79feec1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_37c937c9-5b17-47f7-a4dc-f806c6150984" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_deec79e9-670f-4c0b-93c8-9c1fd79feec1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_ProductTechnologiesMember_1467fe47-d0cc-45cf-b65c-6f76bd0832aa" xlink:href="ango-20200831.xsd#ango_ProductTechnologiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_deec79e9-670f-4c0b-93c8-9c1fd79feec1" xlink:to="loc_ango_ProductTechnologiesMember_1467fe47-d0cc-45cf-b65c-6f76bd0832aa" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerRelationshipsMember_d1d10327-67af-452a-b62a-07274e664885" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CustomerRelationshipsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_deec79e9-670f-4c0b-93c8-9c1fd79feec1" xlink:to="loc_us-gaap_CustomerRelationshipsMember_d1d10327-67af-452a-b62a-07274e664885" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TrademarksMember_9022ceea-8108-44d5-b9a3-9325a3d52346" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TrademarksMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_deec79e9-670f-4c0b-93c8-9c1fd79feec1" xlink:to="loc_us-gaap_TrademarksMember_9022ceea-8108-44d5-b9a3-9325a3d52346" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LicenseAgreementTermsMember_d2cf44bc-cc64-44be-994f-208a6c0b8d26" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LicenseAgreementTermsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_deec79e9-670f-4c0b-93c8-9c1fd79feec1" xlink:to="loc_us-gaap_LicenseAgreementTermsMember_d2cf44bc-cc64-44be-994f-208a6c0b8d26" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/GoodwillandIntangibleAssetsExpectedFutureAmortizationExpenseDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#GoodwillandIntangibleAssetsExpectedFutureAmortizationExpenseDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/GoodwillandIntangibleAssetsExpectedFutureAmortizationExpenseDetail" xlink:type="extended" id="if8e14655fd1547d1987bca672ab81ff5_GoodwillandIntangibleAssetsExpectedFutureAmortizationExpenseDetail"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AccruedLiabilities" xlink:type="simple" xlink:href="ango-20200831.xsd#AccruedLiabilities"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AccruedLiabilities" xlink:type="extended" id="ie5a16c1ea8f34e7aba321b64c43eea2f_AccruedLiabilities"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AccruedLiabilitiesTables" xlink:type="simple" xlink:href="ango-20200831.xsd#AccruedLiabilitiesTables"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AccruedLiabilitiesTables" xlink:type="extended" id="i7ef8594143c543039e91c7c0da5277bb_AccruedLiabilitiesTables"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AccruedLiabilitiesSummaryofAccruedLiabilitiesDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#AccruedLiabilitiesSummaryofAccruedLiabilitiesDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AccruedLiabilitiesSummaryofAccruedLiabilitiesDetail" xlink:type="extended" id="iacb8bb6c22c1419da0265a0c6be7a2e6_AccruedLiabilitiesSummaryofAccruedLiabilitiesDetail"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/LongTermDebt" xlink:type="simple" xlink:href="ango-20200831.xsd#LongTermDebt"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/LongTermDebt" xlink:type="extended" id="i0d305bb4456347fb904886290f819b9d_LongTermDebt"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/LongTermDebtNarrativeDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#LongTermDebtNarrativeDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/LongTermDebtNarrativeDetail" xlink:type="extended" id="id4d1cbbcd15648edb3973c5247dc7f51_LongTermDebtNarrativeDetail">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_7511a7fd-cdc4-4492-ba34-d43aaf996f95" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_65b2d948-b501-432a-a8e2-bc7d7b2f0135" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_7511a7fd-cdc4-4492-ba34-d43aaf996f95" xlink:to="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_65b2d948-b501-432a-a8e2-bc7d7b2f0135" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_LineOfCreditFacilityMaximumBorrowingCapacityAccordianFeature_168a2e54-e151-4d91-a703-4fd6c8072c31" xlink:href="ango-20200831.xsd#ango_LineOfCreditFacilityMaximumBorrowingCapacityAccordianFeature"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_7511a7fd-cdc4-4492-ba34-d43aaf996f95" xlink:to="loc_ango_LineOfCreditFacilityMaximumBorrowingCapacityAccordianFeature_168a2e54-e151-4d91-a703-4fd6c8072c31" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_d6e5204c-11e3-408f-aa47-6c8cf79d5ebf" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_7511a7fd-cdc4-4492-ba34-d43aaf996f95" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_d6e5204c-11e3-408f-aa47-6c8cf79d5ebf" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_DebtInstrumentDebtDefaultInterestRateIncrease_7e9cf867-266f-4179-a8bc-0d3d8cb71453" xlink:href="ango-20200831.xsd#ango_DebtInstrumentDebtDefaultInterestRateIncrease"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_7511a7fd-cdc4-4492-ba34-d43aaf996f95" xlink:to="loc_ango_DebtInstrumentDebtDefaultInterestRateIncrease_7e9cf867-266f-4179-a8bc-0d3d8cb71453" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityCommitmentFeePercentage_cec59430-12a4-4cc7-a158-667757da061e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityCommitmentFeePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_7511a7fd-cdc4-4492-ba34-d43aaf996f95" xlink:to="loc_us-gaap_LineOfCreditFacilityCommitmentFeePercentage_cec59430-12a4-4cc7-a158-667757da061e" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_LineOfCreditFacilityNumberOfCovenants_b45e2ee1-5794-42ff-9af0-7c4be82d7760" xlink:href="ango-20200831.xsd#ango_LineOfCreditFacilityNumberOfCovenants"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_7511a7fd-cdc4-4492-ba34-d43aaf996f95" xlink:to="loc_ango_LineOfCreditFacilityNumberOfCovenants_b45e2ee1-5794-42ff-9af0-7c4be82d7760" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_DebtInstrumentCovenantTermsMaximumTotalLeverageRatio_b1e95248-0fe6-4145-8df1-464cccfc987e" xlink:href="ango-20200831.xsd#ango_DebtInstrumentCovenantTermsMaximumTotalLeverageRatio"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_7511a7fd-cdc4-4492-ba34-d43aaf996f95" xlink:to="loc_ango_DebtInstrumentCovenantTermsMaximumTotalLeverageRatio_b1e95248-0fe6-4145-8df1-464cccfc987e" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_DebtInstrumentCovenantTermsMaximumTotalLeverageRatioSubsequentToMaterialAcquisitions_4b7ad4fa-4433-484e-8115-362095f5ed0d" xlink:href="ango-20200831.xsd#ango_DebtInstrumentCovenantTermsMaximumTotalLeverageRatioSubsequentToMaterialAcquisitions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_7511a7fd-cdc4-4492-ba34-d43aaf996f95" xlink:to="loc_ango_DebtInstrumentCovenantTermsMaximumTotalLeverageRatioSubsequentToMaterialAcquisitions_4b7ad4fa-4433-484e-8115-362095f5ed0d" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_DebtInstrumentCovenantTermsMinimumFixedChargeCoverageRatio_0adf6109-249a-4fe7-aefc-ca2ee93b7d79" xlink:href="ango-20200831.xsd#ango_DebtInstrumentCovenantTermsMinimumFixedChargeCoverageRatio"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_7511a7fd-cdc4-4492-ba34-d43aaf996f95" xlink:to="loc_ango_DebtInstrumentCovenantTermsMinimumFixedChargeCoverageRatio_0adf6109-249a-4fe7-aefc-ca2ee93b7d79" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCredit_2fd55289-b3c2-47c0-8509-e501897cf383" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCredit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_7511a7fd-cdc4-4492-ba34-d43aaf996f95" xlink:to="loc_us-gaap_LineOfCredit_2fd55289-b3c2-47c0-8509-e501897cf383" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityInterestRateAtPeriodEnd_95a24fd0-abba-4237-a4e7-9f8b9424c0b0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityInterestRateAtPeriodEnd"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_7511a7fd-cdc4-4492-ba34-d43aaf996f95" xlink:to="loc_us-gaap_LineOfCreditFacilityInterestRateAtPeriodEnd_95a24fd0-abba-4237-a4e7-9f8b9424c0b0" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_2a3647fc-727e-4f7a-9872-07d0246ebe81" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_7511a7fd-cdc4-4492-ba34-d43aaf996f95" xlink:to="loc_us-gaap_DebtInstrumentTable_2a3647fc-727e-4f7a-9872-07d0246ebe81" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_22eda838-85c3-4bb1-8849-607d3d15dd9f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_2a3647fc-727e-4f7a-9872-07d0246ebe81" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_22eda838-85c3-4bb1-8849-607d3d15dd9f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_22eda838-85c3-4bb1-8849-607d3d15dd9f_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_22eda838-85c3-4bb1-8849-607d3d15dd9f" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_22eda838-85c3-4bb1-8849-607d3d15dd9f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_d0e5d2e7-2da2-4ee5-a4ef-5886ef111e52" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_22eda838-85c3-4bb1-8849-607d3d15dd9f" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_d0e5d2e7-2da2-4ee5-a4ef-5886ef111e52" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_c7f699b3-39bd-4b35-a386-dae695bff5c2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_d0e5d2e7-2da2-4ee5-a4ef-5886ef111e52" xlink:to="loc_us-gaap_LineOfCreditMember_c7f699b3-39bd-4b35-a386-dae695bff5c2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_5122721a-45ec-4eb3-9ec0-b147e481380a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_2a3647fc-727e-4f7a-9872-07d0246ebe81" xlink:to="loc_us-gaap_CreditFacilityAxis_5122721a-45ec-4eb3-9ec0-b147e481380a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_5122721a-45ec-4eb3-9ec0-b147e481380a_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_5122721a-45ec-4eb3-9ec0-b147e481380a" xlink:to="loc_us-gaap_CreditFacilityDomain_5122721a-45ec-4eb3-9ec0-b147e481380a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_2540fda5-b745-496a-9e33-249ec79edd93" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_5122721a-45ec-4eb3-9ec0-b147e481380a" xlink:to="loc_us-gaap_CreditFacilityDomain_2540fda5-b745-496a-9e33-249ec79edd93" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevolvingCreditFacilityMember_44824160-67ca-450f-b7e4-29016f26ad14" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_2540fda5-b745-496a-9e33-249ec79edd93" xlink:to="loc_us-gaap_RevolvingCreditFacilityMember_44824160-67ca-450f-b7e4-29016f26ad14" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_ebd08cc2-e000-4162-bf0e-16b0bbb9cd4f" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_2a3647fc-727e-4f7a-9872-07d0246ebe81" xlink:to="loc_srt_RangeAxis_ebd08cc2-e000-4162-bf0e-16b0bbb9cd4f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_ebd08cc2-e000-4162-bf0e-16b0bbb9cd4f_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_ebd08cc2-e000-4162-bf0e-16b0bbb9cd4f" xlink:to="loc_srt_RangeMember_ebd08cc2-e000-4162-bf0e-16b0bbb9cd4f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_983ca38f-f9bb-4770-8681-0807240085b1" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_ebd08cc2-e000-4162-bf0e-16b0bbb9cd4f" xlink:to="loc_srt_RangeMember_983ca38f-f9bb-4770-8681-0807240085b1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_bc999211-50b7-4128-8bec-d58347442928" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_983ca38f-f9bb-4770-8681-0807240085b1" xlink:to="loc_srt_MinimumMember_bc999211-50b7-4128-8bec-d58347442928" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_802b9247-ba6a-48ab-ac5d-8ff8b5ce0c45" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_983ca38f-f9bb-4770-8681-0807240085b1" xlink:to="loc_srt_MaximumMember_802b9247-ba6a-48ab-ac5d-8ff8b5ce0c45" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateAxis_47cb3fb1-e29a-4742-8187-c3eadc881450" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VariableRateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_2a3647fc-727e-4f7a-9872-07d0246ebe81" xlink:to="loc_us-gaap_VariableRateAxis_47cb3fb1-e29a-4742-8187-c3eadc881450" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_47cb3fb1-e29a-4742-8187-c3eadc881450_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VariableRateAxis_47cb3fb1-e29a-4742-8187-c3eadc881450" xlink:to="loc_us-gaap_VariableRateDomain_47cb3fb1-e29a-4742-8187-c3eadc881450_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_29d086ed-2f7b-4dd5-a8aa-fcb7be1c5435" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VariableRateAxis_47cb3fb1-e29a-4742-8187-c3eadc881450" xlink:to="loc_us-gaap_VariableRateDomain_29d086ed-2f7b-4dd5-a8aa-fcb7be1c5435" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BaseRateMember_38ec6c71-2b91-46cd-b85a-f5c4e93e1363" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BaseRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_29d086ed-2f7b-4dd5-a8aa-fcb7be1c5435" xlink:to="loc_us-gaap_BaseRateMember_38ec6c71-2b91-46cd-b85a-f5c4e93e1363" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LondonInterbankOfferedRateLIBORMember_9bec9f8f-968c-411e-8906-b02cd2468ea2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LondonInterbankOfferedRateLIBORMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_29d086ed-2f7b-4dd5-a8aa-fcb7be1c5435" xlink:to="loc_us-gaap_LondonInterbankOfferedRateLIBORMember_9bec9f8f-968c-411e-8906-b02cd2468ea2" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/IncomeTaxes" xlink:type="simple" xlink:href="ango-20200831.xsd#IncomeTaxes"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/IncomeTaxes" xlink:type="extended" id="i5136e8d27f9a48d394b9e6c23a11c89a_IncomeTaxes"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/IncomeTaxesAdditionalInformationDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#IncomeTaxesAdditionalInformationDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/IncomeTaxesAdditionalInformationDetail" xlink:type="extended" id="i1241a07d6e554235b17b0e587348b5b7_IncomeTaxesAdditionalInformationDetail"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/ShareBasedCompensation" xlink:type="simple" xlink:href="ango-20200831.xsd#ShareBasedCompensation"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/ShareBasedCompensation" xlink:type="extended" id="ibec79f45e5994de98a92d1d601c1234d_ShareBasedCompensation"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/ShareBasedCompensationNarrativeDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#ShareBasedCompensationNarrativeDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/ShareBasedCompensationNarrativeDetail" xlink:type="extended" id="i9fb8008215fd4821b798c787a5c23293_ShareBasedCompensationNarrativeDetail">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a17f0afc-1165-4352-ac79-4b6e5d35b953" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized_6d42263f-721b-41ad-bb9a-8cc65ccf2b51" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a17f0afc-1165-4352-ac79-4b6e5d35b953" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized_6d42263f-721b-41ad-bb9a-8cc65ccf2b51" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_9877382d-e0ef-4900-90eb-2e49a9e6bfa5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a17f0afc-1165-4352-ac79-4b6e5d35b953" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_9877382d-e0ef-4900-90eb-2e49a9e6bfa5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_ce7c3793-af88-4e69-86a8-d5e1bd6a65c6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a17f0afc-1165-4352-ac79-4b6e5d35b953" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_ce7c3793-af88-4e69-86a8-d5e1bd6a65c6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_66763537-255b-4627-9e32-3ecddaa34984" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a17f0afc-1165-4352-ac79-4b6e5d35b953" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_66763537-255b-4627-9e32-3ecddaa34984" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_1f1a9cf3-7ae3-4df8-b16b-d29b8614e437" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a17f0afc-1165-4352-ac79-4b6e5d35b953" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_1f1a9cf3-7ae3-4df8-b16b-d29b8614e437" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_44e40d32-92a3-4f72-9ede-35b6214e084b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_1f1a9cf3-7ae3-4df8-b16b-d29b8614e437" xlink:to="loc_us-gaap_PlanNameAxis_44e40d32-92a3-4f72-9ede-35b6214e084b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_44e40d32-92a3-4f72-9ede-35b6214e084b_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_44e40d32-92a3-4f72-9ede-35b6214e084b" xlink:to="loc_us-gaap_PlanNameDomain_44e40d32-92a3-4f72-9ede-35b6214e084b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_6aa88e0d-d674-451c-aaeb-b507ca74daf5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_44e40d32-92a3-4f72-9ede-35b6214e084b" xlink:to="loc_us-gaap_PlanNameDomain_6aa88e0d-d674-451c-aaeb-b507ca74daf5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_The2004PlanandEmployeeStockPurchasePlanMember_50b69879-d320-4da0-b63e-1cb978f30d7d" xlink:href="ango-20200831.xsd#ango_The2004PlanandEmployeeStockPurchasePlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_6aa88e0d-d674-451c-aaeb-b507ca74daf5" xlink:to="loc_ango_The2004PlanandEmployeeStockPurchasePlanMember_50b69879-d320-4da0-b63e-1cb978f30d7d" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/EarningsPerShare" xlink:type="simple" xlink:href="ango-20200831.xsd#EarningsPerShare"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/EarningsPerShare" xlink:type="extended" id="i4d0a2f37498f4d659acc28e7e290b040_EarningsPerShare"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/EarningsPerShareTables" xlink:type="simple" xlink:href="ango-20200831.xsd#EarningsPerShareTables"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/EarningsPerShareTables" xlink:type="extended" id="ib74433a8a38346d69cf65b038151480c_EarningsPerShareTables"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/EarningsPerShareReconciliationofBasictoDilutedWeightedAverageSharesOutstandingDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#EarningsPerShareReconciliationofBasictoDilutedWeightedAverageSharesOutstandingDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/EarningsPerShareReconciliationofBasictoDilutedWeightedAverageSharesOutstandingDetail" xlink:type="extended" id="i1a1bbd8ff18e432abca945090ae61990_EarningsPerShareReconciliationofBasictoDilutedWeightedAverageSharesOutstandingDetail"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/SegmentandGeographicInformation" xlink:type="simple" xlink:href="ango-20200831.xsd#SegmentandGeographicInformation"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/SegmentandGeographicInformation" xlink:type="extended" id="id8c1a5c791c14c7abc7812d17b1a92c9_SegmentandGeographicInformation"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/SegmentandGeographicInformationTables" xlink:type="simple" xlink:href="ango-20200831.xsd#SegmentandGeographicInformationTables"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/SegmentandGeographicInformationTables" xlink:type="extended" id="i11c222e4c46247d9952161b77a9599b5_SegmentandGeographicInformationTables"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/SegmentandGeographicInformationSummaryofNetSalesbyProductCategoryDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#SegmentandGeographicInformationSummaryofNetSalesbyProductCategoryDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/SegmentandGeographicInformationSummaryofNetSalesbyProductCategoryDetail" xlink:type="extended" id="i4ef505c5d3884ce088c88e3b0db59ae7_SegmentandGeographicInformationSummaryofNetSalesbyProductCategoryDetail">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_87b178ce-54fb-4074-b50b-1f40cdda9ff2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Revenues_100207c2-0f53-489e-8d80-c95267f0b27e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_Revenues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_87b178ce-54fb-4074-b50b-1f40cdda9ff2" xlink:to="loc_us-gaap_Revenues_100207c2-0f53-489e-8d80-c95267f0b27e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_ae86c3a4-49b7-4d46-bee5-75da9131653f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_87b178ce-54fb-4074-b50b-1f40cdda9ff2" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_ae86c3a4-49b7-4d46-bee5-75da9131653f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_20d40994-0896-4d2c-8819-cb40c6713972" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_ae86c3a4-49b7-4d46-bee5-75da9131653f" xlink:to="loc_srt_ProductOrServiceAxis_20d40994-0896-4d2c-8819-cb40c6713972" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_20d40994-0896-4d2c-8819-cb40c6713972_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_20d40994-0896-4d2c-8819-cb40c6713972" xlink:to="loc_srt_ProductsAndServicesDomain_20d40994-0896-4d2c-8819-cb40c6713972_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_c744ca1f-52ae-4ad0-95f0-97b9d6a8a87f" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_20d40994-0896-4d2c-8819-cb40c6713972" xlink:to="loc_srt_ProductsAndServicesDomain_c744ca1f-52ae-4ad0-95f0-97b9d6a8a87f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_VascularInterventionsandTherapiesMember_3eea9756-bd31-4841-bebc-581305b6135e" xlink:href="ango-20200831.xsd#ango_VascularInterventionsandTherapiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_c744ca1f-52ae-4ad0-95f0-97b9d6a8a87f" xlink:to="loc_ango_VascularInterventionsandTherapiesMember_3eea9756-bd31-4841-bebc-581305b6135e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_VascularAccessMember_50e21619-e84f-4a88-b6bf-038b5087fb35" xlink:href="ango-20200831.xsd#ango_VascularAccessMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_c744ca1f-52ae-4ad0-95f0-97b9d6a8a87f" xlink:to="loc_ango_VascularAccessMember_50e21619-e84f-4a88-b6bf-038b5087fb35" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_OncologyMember_3ec8dc9b-2fdd-4fb8-8329-c82c6c3c21d5" xlink:href="ango-20200831.xsd#ango_OncologyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_c744ca1f-52ae-4ad0-95f0-97b9d6a8a87f" xlink:to="loc_ango_OncologyMember_3ec8dc9b-2fdd-4fb8-8329-c82c6c3c21d5" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/SegmentandGeographicInformationSummaryofNetSalesbyGeographicAreaDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#SegmentandGeographicInformationSummaryofNetSalesbyGeographicAreaDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/SegmentandGeographicInformationSummaryofNetSalesbyGeographicAreaDetail" xlink:type="extended" id="i26a7c41c685a4ab787de43d24e5e9f91_SegmentandGeographicInformationSummaryofNetSalesbyGeographicAreaDetail">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_496ec589-a0ce-4751-a88b-0e1434703ecc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Revenues_54309fbc-86b7-4236-a7bd-772e18240da1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_Revenues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_496ec589-a0ce-4751-a88b-0e1434703ecc" xlink:to="loc_us-gaap_Revenues_54309fbc-86b7-4236-a7bd-772e18240da1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_3e07518e-c377-4fb3-b4e2-d272213bf512" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_496ec589-a0ce-4751-a88b-0e1434703ecc" xlink:to="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_3e07518e-c377-4fb3-b4e2-d272213bf512" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_bc0bb4de-5bc0-40fe-873a-ff70021db0c6" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_3e07518e-c377-4fb3-b4e2-d272213bf512" xlink:to="loc_srt_StatementGeographicalAxis_bc0bb4de-5bc0-40fe-873a-ff70021db0c6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_bc0bb4de-5bc0-40fe-873a-ff70021db0c6_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_bc0bb4de-5bc0-40fe-873a-ff70021db0c6" xlink:to="loc_srt_SegmentGeographicalDomain_bc0bb4de-5bc0-40fe-873a-ff70021db0c6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_72f693f6-90e3-4db9-b033-03cef16d783d" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_bc0bb4de-5bc0-40fe-873a-ff70021db0c6" xlink:to="loc_srt_SegmentGeographicalDomain_72f693f6-90e3-4db9-b033-03cef16d783d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_3650ad0f-87d2-49c5-bf7a-26826d949f05" xlink:href="https://xbrl.sec.gov/country/2020/country-2020-01-31.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_72f693f6-90e3-4db9-b033-03cef16d783d" xlink:to="loc_country_US_3650ad0f-87d2-49c5-bf7a-26826d949f05" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonUsMember_7fc24ba8-ad5a-4908-af81-696e5a2d0157" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NonUsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_72f693f6-90e3-4db9-b033-03cef16d783d" xlink:to="loc_us-gaap_NonUsMember_7fc24ba8-ad5a-4908-af81-696e5a2d0157" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/FairValue" xlink:type="simple" xlink:href="ango-20200831.xsd#FairValue"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/FairValue" xlink:type="extended" id="if6d559a9406d44b08f10c5105b74e8dc_FairValue"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/FairValueTables" xlink:type="simple" xlink:href="ango-20200831.xsd#FairValueTables"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/FairValueTables" xlink:type="extended" id="iafecb78175b54d20aaea2e6a8865bd69_FairValueTables"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/FairValueFairValueofAssetsandLiabilitiesMeasuredonaRecurringBasisDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#FairValueFairValueofAssetsandLiabilitiesMeasuredonaRecurringBasisDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/FairValueFairValueofAssetsandLiabilitiesMeasuredonaRecurringBasisDetail" xlink:type="extended" id="i1e8af8a274714817886642f47968df9c_FairValueFairValueofAssetsandLiabilitiesMeasuredonaRecurringBasisDetail">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_e141135b-be19-4366-8f8d-e2dc6d2ae0d6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_99038576-bdcd-414b-a8b2-009f6af37baa" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LiabilitiesFairValueDisclosureAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_e141135b-be19-4366-8f8d-e2dc6d2ae0d6" xlink:to="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_99038576-bdcd-414b-a8b2-009f6af37baa" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_b5fc0620-fabc-4c0c-8235-fd2ef05b08d8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationContingentConsiderationLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_99038576-bdcd-414b-a8b2-009f6af37baa" xlink:to="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_b5fc0620-fabc-4c0c-8235-fd2ef05b08d8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesFairValueDisclosure_52fd8cd7-6528-44e2-9c5b-61cd1bf08fa8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LiabilitiesFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_99038576-bdcd-414b-a8b2-009f6af37baa" xlink:to="loc_us-gaap_LiabilitiesFairValueDisclosure_52fd8cd7-6528-44e2-9c5b-61cd1bf08fa8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_9e3bef40-4c33-446e-9ba6-0faeeb0444a4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_e141135b-be19-4366-8f8d-e2dc6d2ae0d6" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_9e3bef40-4c33-446e-9ba6-0faeeb0444a4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_4b5249bb-b6c4-4c62-8cd1-7533e5aae8f0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_9e3bef40-4c33-446e-9ba6-0faeeb0444a4" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_4b5249bb-b6c4-4c62-8cd1-7533e5aae8f0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_4b5249bb-b6c4-4c62-8cd1-7533e5aae8f0_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_4b5249bb-b6c4-4c62-8cd1-7533e5aae8f0" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_4b5249bb-b6c4-4c62-8cd1-7533e5aae8f0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2a85b86a-c8c8-4afe-98af-249a1d0b261b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_4b5249bb-b6c4-4c62-8cd1-7533e5aae8f0" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2a85b86a-c8c8-4afe-98af-249a1d0b261b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_726abed1-bdd0-4861-9441-b6d580249ad6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2a85b86a-c8c8-4afe-98af-249a1d0b261b" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_726abed1-bdd0-4861-9441-b6d580249ad6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_8c028709-3ccc-4e2c-b24e-15062ff554a3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2a85b86a-c8c8-4afe-98af-249a1d0b261b" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_8c028709-3ccc-4e2c-b24e-15062ff554a3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_04f2e468-c3a6-4831-a133-3b9a495ebd90" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2a85b86a-c8c8-4afe-98af-249a1d0b261b" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_04f2e468-c3a6-4831-a133-3b9a495ebd90" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_fb2eafb6-c969-494e-8513-ee8c5bc6394b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_9e3bef40-4c33-446e-9ba6-0faeeb0444a4" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_fb2eafb6-c969-494e-8513-ee8c5bc6394b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_fb2eafb6-c969-494e-8513-ee8c5bc6394b_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_fb2eafb6-c969-494e-8513-ee8c5bc6394b" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_fb2eafb6-c969-494e-8513-ee8c5bc6394b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_a3aba1a9-5140-4847-880f-80fe1fe2a907" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_fb2eafb6-c969-494e-8513-ee8c5bc6394b" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_a3aba1a9-5140-4847-880f-80fe1fe2a907" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsRecurringMember_f8974100-28a1-4810-93c5-8e64de781f7d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsRecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_a3aba1a9-5140-4847-880f-80fe1fe2a907" xlink:to="loc_us-gaap_FairValueMeasurementsRecurringMember_f8974100-28a1-4810-93c5-8e64de781f7d" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/FairValueFairValueMeasurementsUsingSignificantUnobservableInputsDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#FairValueFairValueMeasurementsUsingSignificantUnobservableInputsDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/FairValueFairValueMeasurementsUsingSignificantUnobservableInputsDetail" xlink:type="extended" id="ia09b015522824379be88cd1b061a534a_FairValueFairValueMeasurementsUsingSignificantUnobservableInputsDetail"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/FairValueSummaryShowingtheRecurringFairValueMeasurementsoftheContingentConsiderationLiabilityDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#FairValueSummaryShowingtheRecurringFairValueMeasurementsoftheContingentConsiderationLiabilityDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/FairValueSummaryShowingtheRecurringFairValueMeasurementsoftheContingentConsiderationLiabilityDetail" xlink:type="extended" id="i73efa746d0164bc0a72bf2c967a67c0d_FairValueSummaryShowingtheRecurringFairValueMeasurementsoftheContingentConsiderationLiabilityDetail">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_a1da1d83-d262-4424-9bab-3c487b4e9e58" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_14a71ebd-1e6c-49f9-ae60-02273a137545" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationContingentConsiderationLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_a1da1d83-d262-4424-9bab-3c487b4e9e58" xlink:to="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_14a71ebd-1e6c-49f9-ae60-02273a137545" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationContingentConsiderationLiabilityMeasurementInput_d6237865-da66-44ce-81a9-117e1a7d8ac9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationContingentConsiderationLiabilityMeasurementInput"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_a1da1d83-d262-4424-9bab-3c487b4e9e58" xlink:to="loc_us-gaap_BusinessCombinationContingentConsiderationLiabilityMeasurementInput_d6237865-da66-44ce-81a9-117e1a7d8ac9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_7c69a579-83d2-4db1-8847-697d30541fdb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_a1da1d83-d262-4424-9bab-3c487b4e9e58" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_7c69a579-83d2-4db1-8847-697d30541fdb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_362b0017-ae82-4e86-a08e-282048ab57b5" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_7c69a579-83d2-4db1-8847-697d30541fdb" xlink:to="loc_srt_RangeAxis_362b0017-ae82-4e86-a08e-282048ab57b5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_362b0017-ae82-4e86-a08e-282048ab57b5_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_362b0017-ae82-4e86-a08e-282048ab57b5" xlink:to="loc_srt_RangeMember_362b0017-ae82-4e86-a08e-282048ab57b5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_447b2485-e2ba-44ed-970a-ac1f5ef66dcd" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_362b0017-ae82-4e86-a08e-282048ab57b5" xlink:to="loc_srt_RangeMember_447b2485-e2ba-44ed-970a-ac1f5ef66dcd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_52e22702-819c-463d-91b6-364f279e2e06" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_447b2485-e2ba-44ed-970a-ac1f5ef66dcd" xlink:to="loc_srt_MinimumMember_52e22702-819c-463d-91b6-364f279e2e06" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_c938a7bf-3df8-4716-ad39-0ba7e6d7b163" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_447b2485-e2ba-44ed-970a-ac1f5ef66dcd" xlink:to="loc_srt_MaximumMember_c938a7bf-3df8-4716-ad39-0ba7e6d7b163" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContingentConsiderationByTypeAxis_9b5743c2-56e4-4e19-ac73-6904976c6398" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContingentConsiderationByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_7c69a579-83d2-4db1-8847-697d30541fdb" xlink:to="loc_us-gaap_ContingentConsiderationByTypeAxis_9b5743c2-56e4-4e19-ac73-6904976c6398" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContingentConsiderationTypeDomain_9b5743c2-56e4-4e19-ac73-6904976c6398_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContingentConsiderationTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ContingentConsiderationByTypeAxis_9b5743c2-56e4-4e19-ac73-6904976c6398" xlink:to="loc_us-gaap_ContingentConsiderationTypeDomain_9b5743c2-56e4-4e19-ac73-6904976c6398_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContingentConsiderationTypeDomain_d79c37f5-0ac3-42ac-af42-6e9241d89898" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContingentConsiderationTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ContingentConsiderationByTypeAxis_9b5743c2-56e4-4e19-ac73-6904976c6398" xlink:to="loc_us-gaap_ContingentConsiderationTypeDomain_d79c37f5-0ac3-42ac-af42-6e9241d89898" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_RevenueBasedPaymentMember_406edda4-1875-48a8-a5a3-da21e05d3e16" xlink:href="ango-20200831.xsd#ango_RevenueBasedPaymentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContingentConsiderationTypeDomain_d79c37f5-0ac3-42ac-af42-6e9241d89898" xlink:to="loc_ango_RevenueBasedPaymentMember_406edda4-1875-48a8-a5a3-da21e05d3e16" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeAxis_aaf91d88-cee4-4d43-9968-203fac65dfa3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MeasurementInputTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_7c69a579-83d2-4db1-8847-697d30541fdb" xlink:to="loc_us-gaap_MeasurementInputTypeAxis_aaf91d88-cee4-4d43-9968-203fac65dfa3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeDomain_aaf91d88-cee4-4d43-9968-203fac65dfa3_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MeasurementInputTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_MeasurementInputTypeAxis_aaf91d88-cee4-4d43-9968-203fac65dfa3" xlink:to="loc_us-gaap_MeasurementInputTypeDomain_aaf91d88-cee4-4d43-9968-203fac65dfa3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeDomain_572a2467-d80c-473d-8d6b-4c2c9e5a98be" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MeasurementInputTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_MeasurementInputTypeAxis_aaf91d88-cee4-4d43-9968-203fac65dfa3" xlink:to="loc_us-gaap_MeasurementInputTypeDomain_572a2467-d80c-473d-8d6b-4c2c9e5a98be" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputDiscountRateMember_5a05c9fc-776c-4985-897e-a2e279da3e08" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MeasurementInputDiscountRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MeasurementInputTypeDomain_572a2467-d80c-473d-8d6b-4c2c9e5a98be" xlink:to="loc_us-gaap_MeasurementInputDiscountRateMember_5a05c9fc-776c-4985-897e-a2e279da3e08" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_MeasurementInputProbabilityofPaymentMember_b5d4c808-7e16-4aee-90c9-e79e11a8d73e" xlink:href="ango-20200831.xsd#ango_MeasurementInputProbabilityofPaymentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MeasurementInputTypeDomain_572a2467-d80c-473d-8d6b-4c2c9e5a98be" xlink:to="loc_ango_MeasurementInputProbabilityofPaymentMember_b5d4c808-7e16-4aee-90c9-e79e11a8d73e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationTechniqueAxis_58c9405f-295e-43c6-8173-66a615a65ec2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ValuationTechniqueAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_7c69a579-83d2-4db1-8847-697d30541fdb" xlink:to="loc_us-gaap_ValuationTechniqueAxis_58c9405f-295e-43c6-8173-66a615a65ec2" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationTechniqueDomain_58c9405f-295e-43c6-8173-66a615a65ec2_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ValuationTechniqueDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ValuationTechniqueAxis_58c9405f-295e-43c6-8173-66a615a65ec2" xlink:to="loc_us-gaap_ValuationTechniqueDomain_58c9405f-295e-43c6-8173-66a615a65ec2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationTechniqueDomain_452bc70f-b04d-45cd-aba9-43ccdd90d821" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ValuationTechniqueDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ValuationTechniqueAxis_58c9405f-295e-43c6-8173-66a615a65ec2" xlink:to="loc_us-gaap_ValuationTechniqueDomain_452bc70f-b04d-45cd-aba9-43ccdd90d821" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationTechniqueDiscountedCashFlowMember_d93d9add-34aa-4e75-824b-e38911169ce3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ValuationTechniqueDiscountedCashFlowMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ValuationTechniqueDomain_452bc70f-b04d-45cd-aba9-43ccdd90d821" xlink:to="loc_us-gaap_ValuationTechniqueDiscountedCashFlowMember_d93d9add-34aa-4e75-824b-e38911169ce3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_55c54456-8ed1-455a-af14-b9f6dbaa0211" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_7c69a579-83d2-4db1-8847-697d30541fdb" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_55c54456-8ed1-455a-af14-b9f6dbaa0211" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_55c54456-8ed1-455a-af14-b9f6dbaa0211_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_55c54456-8ed1-455a-af14-b9f6dbaa0211" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_55c54456-8ed1-455a-af14-b9f6dbaa0211_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_e0f48657-b398-471a-a2c2-ad1ffc04732a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_55c54456-8ed1-455a-af14-b9f6dbaa0211" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_e0f48657-b398-471a-a2c2-ad1ffc04732a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_93579505-0e14-442d-8fb4-716e3fd76deb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_e0f48657-b398-471a-a2c2-ad1ffc04732a" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_93579505-0e14-442d-8fb4-716e3fd76deb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_7afd79b7-f3bc-4b96-8df3-18ffaa184450" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_7c69a579-83d2-4db1-8847-697d30541fdb" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_7afd79b7-f3bc-4b96-8df3-18ffaa184450" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_7afd79b7-f3bc-4b96-8df3-18ffaa184450_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_7afd79b7-f3bc-4b96-8df3-18ffaa184450" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_7afd79b7-f3bc-4b96-8df3-18ffaa184450_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_bf72c6f0-ff4b-4453-a2b7-4d160d50436c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_7afd79b7-f3bc-4b96-8df3-18ffaa184450" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_bf72c6f0-ff4b-4453-a2b7-4d160d50436c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsRecurringMember_f37cd430-4392-4e1f-8427-de893a28de62" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsRecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_bf72c6f0-ff4b-4453-a2b7-4d160d50436c" xlink:to="loc_us-gaap_FairValueMeasurementsRecurringMember_f37cd430-4392-4e1f-8427-de893a28de62" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/FairValueAdditionalInformationDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#FairValueAdditionalInformationDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/FairValueAdditionalInformationDetail" xlink:type="extended" id="i9731e07be36d41b0aa2038ab1a384b40_FairValueAdditionalInformationDetail"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/Leases" xlink:type="simple" xlink:href="ango-20200831.xsd#Leases"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/Leases" xlink:type="extended" id="i492059be66df41469341f4487f2b9d48_Leases"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/LeasesTables" xlink:type="simple" xlink:href="ango-20200831.xsd#LeasesTables"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/LeasesTables" xlink:type="extended" id="id28eb3786a8c43ff87c75ca10f335a1b_LeasesTables"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/LeasesSupplementalBalanceSheetInformationDetails" xlink:type="simple" xlink:href="ango-20200831.xsd#LeasesSupplementalBalanceSheetInformationDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/LeasesSupplementalBalanceSheetInformationDetails" xlink:type="extended" id="i5f2e96e10d5840cda2fc8d98ea9e42fb_LeasesSupplementalBalanceSheetInformationDetails"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/LeasesLiabilityMaturityScheduleDetails" xlink:type="simple" xlink:href="ango-20200831.xsd#LeasesLiabilityMaturityScheduleDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/LeasesLiabilityMaturityScheduleDetails" xlink:type="extended" id="i357d8438dba24af7ac7c4795f37b3917_LeasesLiabilityMaturityScheduleDetails"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/LeasesSupplementalStatementofOperationsInformationDetails" xlink:type="simple" xlink:href="ango-20200831.xsd#LeasesSupplementalStatementofOperationsInformationDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/LeasesSupplementalStatementofOperationsInformationDetails" xlink:type="extended" id="i63878972b3054d268a46d11cc503a812_LeasesSupplementalStatementofOperationsInformationDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeLeaseDescriptionLineItems_04b7683e-e72a-4bc8-be5a-0167df48b4cd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LesseeLeaseDescriptionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseExpense_ad7ee8f2-08ab-4386-b022-776d3c5519e4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingLeaseExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_04b7683e-e72a-4bc8-be5a-0167df48b4cd" xlink:to="loc_us-gaap_OperatingLeaseExpense_ad7ee8f2-08ab-4386-b022-776d3c5519e4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeLeaseDescriptionTable_35a65a2f-de07-41f4-9f07-6e952402bcfa" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LesseeLeaseDescriptionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_04b7683e-e72a-4bc8-be5a-0167df48b4cd" xlink:to="loc_us-gaap_LesseeLeaseDescriptionTable_35a65a2f-de07-41f4-9f07-6e952402bcfa" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_8cb465e7-03bc-48ec-acd5-41b16aa40615" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LesseeLeaseDescriptionTable_35a65a2f-de07-41f4-9f07-6e952402bcfa" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_8cb465e7-03bc-48ec-acd5-41b16aa40615" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_8cb465e7-03bc-48ec-acd5-41b16aa40615_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_8cb465e7-03bc-48ec-acd5-41b16aa40615" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_8cb465e7-03bc-48ec-acd5-41b16aa40615_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_e751bc56-955b-42aa-a17d-a51586b1c43e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_8cb465e7-03bc-48ec-acd5-41b16aa40615" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_e751bc56-955b-42aa-a17d-a51586b1c43e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfSalesMember_843130f3-0aa7-4583-bfa0-3f85a841b50b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CostOfSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_e751bc56-955b-42aa-a17d-a51586b1c43e" xlink:to="loc_us-gaap_CostOfSalesMember_843130f3-0aa7-4583-bfa0-3f85a841b50b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpenseMember_7b2283eb-2b10-451e-a6fe-24e9b7c47f12" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ResearchAndDevelopmentExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_e751bc56-955b-42aa-a17d-a51586b1c43e" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpenseMember_7b2283eb-2b10-451e-a6fe-24e9b7c47f12" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingAndMarketingExpenseMember_af92ea89-afd0-4225-a4e3-00f61fafac4d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SellingAndMarketingExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_e751bc56-955b-42aa-a17d-a51586b1c43e" xlink:to="loc_us-gaap_SellingAndMarketingExpenseMember_af92ea89-afd0-4225-a4e3-00f61fafac4d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpenseMember_f056916c-e08a-4cbc-b401-1c47a26416d2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GeneralAndAdministrativeExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_e751bc56-955b-42aa-a17d-a51586b1c43e" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpenseMember_f056916c-e08a-4cbc-b401-1c47a26416d2" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/LeasesSupplementalCashFlowInformationDetails" xlink:type="simple" xlink:href="ango-20200831.xsd#LeasesSupplementalCashFlowInformationDetails"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/LeasesSupplementalCashFlowInformationDetails" xlink:type="extended" id="i00ac607f66aa479eb078011cdf491dbc_LeasesSupplementalCashFlowInformationDetails"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/CommitmentsandContingencies" xlink:type="simple" xlink:href="ango-20200831.xsd#CommitmentsandContingencies"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/CommitmentsandContingencies" xlink:type="extended" id="i9d06cf0cd7d04b348584e95c1721c0cb_CommitmentsandContingencies"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/CommitmentsandCoontingenciesAdditionalInformationDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#CommitmentsandCoontingenciesAdditionalInformationDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/CommitmentsandCoontingenciesAdditionalInformationDetail" xlink:type="extended" id="ia8587184f8b94f66be56df4d58cd26cb_CommitmentsandCoontingenciesAdditionalInformationDetail">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingenciesLineItems_75840018-73a1-4c8e-b7d0-02ec3eab556a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LossContingenciesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_NumberOfPatentsAssertedForReexamination_5a298242-dc1b-4dec-b71c-d43891f6424c" xlink:href="ango-20200831.xsd#ango_NumberOfPatentsAssertedForReexamination"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_75840018-73a1-4c8e-b7d0-02ec3eab556a" xlink:to="loc_ango_NumberOfPatentsAssertedForReexamination_5a298242-dc1b-4dec-b71c-d43891f6424c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyClaimsDismissedNumber_e93807ec-decf-4d68-b908-b925b80bc983" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LossContingencyClaimsDismissedNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_75840018-73a1-4c8e-b7d0-02ec3eab556a" xlink:to="loc_us-gaap_LossContingencyClaimsDismissedNumber_e93807ec-decf-4d68-b908-b925b80bc983" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_LossContingencyReexaminationsNumber_c31aa8bf-6cd3-43de-9268-136d1308e738" xlink:href="ango-20200831.xsd#ango_LossContingencyReexaminationsNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_75840018-73a1-4c8e-b7d0-02ec3eab556a" xlink:to="loc_ango_LossContingencyReexaminationsNumber_c31aa8bf-6cd3-43de-9268-136d1308e738" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyPendingClaimsNumber_a35cead8-8cdc-4daf-a8de-0d9bb106e71b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LossContingencyPendingClaimsNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_75840018-73a1-4c8e-b7d0-02ec3eab556a" xlink:to="loc_us-gaap_LossContingencyPendingClaimsNumber_a35cead8-8cdc-4daf-a8de-0d9bb106e71b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_LossContingencyClaimsReversedNumber_01317478-773b-492d-baa3-0e3d40aa545a" xlink:href="ango-20200831.xsd#ango_LossContingencyClaimsReversedNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_75840018-73a1-4c8e-b7d0-02ec3eab556a" xlink:to="loc_ango_LossContingencyClaimsReversedNumber_01317478-773b-492d-baa3-0e3d40aa545a" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyPatentsAllegedlyInfringedNumber_de3b70d1-5d90-4169-8862-897e7ec1245a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LossContingencyPatentsAllegedlyInfringedNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_75840018-73a1-4c8e-b7d0-02ec3eab556a" xlink:to="loc_us-gaap_LossContingencyPatentsAllegedlyInfringedNumber_de3b70d1-5d90-4169-8862-897e7ec1245a" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsForLegalSettlements_611283c6-9e6d-48a8-ba87-9d08fe79efa9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentsForLegalSettlements"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_75840018-73a1-4c8e-b7d0-02ec3eab556a" xlink:to="loc_us-gaap_PaymentsForLegalSettlements_611283c6-9e6d-48a8-ba87-9d08fe79efa9" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingenciesTable_6b583b06-069d-4dcc-8a17-6ba444edbdc9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LossContingenciesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LossContingenciesLineItems_75840018-73a1-4c8e-b7d0-02ec3eab556a" xlink:to="loc_us-gaap_LossContingenciesTable_6b583b06-069d-4dcc-8a17-6ba444edbdc9" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_LitigationCaseAxis_067343a4-5a09-4885-9f05-d82bed5e82e2" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_LitigationCaseAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LossContingenciesTable_6b583b06-069d-4dcc-8a17-6ba444edbdc9" xlink:to="loc_srt_LitigationCaseAxis_067343a4-5a09-4885-9f05-d82bed5e82e2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_LitigationCaseTypeDomain_067343a4-5a09-4885-9f05-d82bed5e82e2_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_LitigationCaseTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_LitigationCaseAxis_067343a4-5a09-4885-9f05-d82bed5e82e2" xlink:to="loc_srt_LitigationCaseTypeDomain_067343a4-5a09-4885-9f05-d82bed5e82e2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_LitigationCaseTypeDomain_24d3ee7f-f4e4-403c-8db7-ddcf8e46831c" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_LitigationCaseTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_LitigationCaseAxis_067343a4-5a09-4885-9f05-d82bed5e82e2" xlink:to="loc_srt_LitigationCaseTypeDomain_24d3ee7f-f4e4-403c-8db7-ddcf8e46831c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_CrBardIncMember_ec35537f-94ab-48d7-ac55-030099bb949f" xlink:href="ango-20200831.xsd#ango_CrBardIncMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_LitigationCaseTypeDomain_24d3ee7f-f4e4-403c-8db7-ddcf8e46831c" xlink:to="loc_ango_CrBardIncMember_ec35537f-94ab-48d7-ac55-030099bb949f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_TheUtahActionMember_3ea31d24-e2f8-447d-b305-7ba5ac5166a1" xlink:href="ango-20200831.xsd#ango_TheUtahActionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_LitigationCaseTypeDomain_24d3ee7f-f4e4-403c-8db7-ddcf8e46831c" xlink:to="loc_ango_TheUtahActionMember_3ea31d24-e2f8-447d-b305-7ba5ac5166a1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_MedCompandSmithsPatentActionMember_1ca4627d-03dd-48f5-a0d8-204c3fbf6d07" xlink:href="ango-20200831.xsd#ango_MedCompandSmithsPatentActionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_LitigationCaseTypeDomain_24d3ee7f-f4e4-403c-8db7-ddcf8e46831c" xlink:to="loc_ango_MedCompandSmithsPatentActionMember_1ca4627d-03dd-48f5-a0d8-204c3fbf6d07" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_TheDelawareActionMember_82f0b08f-30ef-4863-b6f8-827240169548" xlink:href="ango-20200831.xsd#ango_TheDelawareActionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_LitigationCaseTypeDomain_24d3ee7f-f4e4-403c-8db7-ddcf8e46831c" xlink:to="loc_ango_TheDelawareActionMember_82f0b08f-30ef-4863-b6f8-827240169548" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_ConstructionIssuesMember_ad488374-ac71-4472-bcf6-c9b4b521ab60" xlink:href="ango-20200831.xsd#ango_ConstructionIssuesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_LitigationCaseTypeDomain_24d3ee7f-f4e4-403c-8db7-ddcf8e46831c" xlink:to="loc_ango_ConstructionIssuesMember_ad488374-ac71-4472-bcf6-c9b4b521ab60" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_MerzNorthAmericaSettlementMember_1dfb01d2-1336-4829-8038-4705b3381203" xlink:href="ango-20200831.xsd#ango_MerzNorthAmericaSettlementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_LitigationCaseTypeDomain_24d3ee7f-f4e4-403c-8db7-ddcf8e46831c" xlink:to="loc_ango_MerzNorthAmericaSettlementMember_1dfb01d2-1336-4829-8038-4705b3381203" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_f7fa4591-1994-4141-aa5c-fa1add3ea554" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LossContingenciesTable_6b583b06-069d-4dcc-8a17-6ba444edbdc9" xlink:to="loc_dei_LegalEntityAxis_f7fa4591-1994-4141-aa5c-fa1add3ea554" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_f7fa4591-1994-4141-aa5c-fa1add3ea554_default" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_f7fa4591-1994-4141-aa5c-fa1add3ea554" xlink:to="loc_dei_EntityDomain_f7fa4591-1994-4141-aa5c-fa1add3ea554_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_4347df1d-f5fa-4fdc-b803-79ea2c03ebb1" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_f7fa4591-1994-4141-aa5c-fa1add3ea554" xlink:to="loc_dei_EntityDomain_4347df1d-f5fa-4fdc-b803-79ea2c03ebb1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_C.R.BardInc.Member_5b8ad55a-f211-44bb-a9a8-2b6175b2d814" xlink:href="ango-20200831.xsd#ango_C.R.BardInc.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_4347df1d-f5fa-4fdc-b803-79ea2c03ebb1" xlink:to="loc_ango_C.R.BardInc.Member_5b8ad55a-f211-44bb-a9a8-2b6175b2d814" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LitigationStatusAxis_9594acfe-dd92-4d1d-8b10-16edd8da23fd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LitigationStatusAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LossContingenciesTable_6b583b06-069d-4dcc-8a17-6ba444edbdc9" xlink:to="loc_us-gaap_LitigationStatusAxis_9594acfe-dd92-4d1d-8b10-16edd8da23fd" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LitigationStatusDomain_9594acfe-dd92-4d1d-8b10-16edd8da23fd_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LitigationStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LitigationStatusAxis_9594acfe-dd92-4d1d-8b10-16edd8da23fd" xlink:to="loc_us-gaap_LitigationStatusDomain_9594acfe-dd92-4d1d-8b10-16edd8da23fd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LitigationStatusDomain_ae217ea8-d0bb-4eea-a27b-438a1bbe3fc8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LitigationStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LitigationStatusAxis_9594acfe-dd92-4d1d-8b10-16edd8da23fd" xlink:to="loc_us-gaap_LitigationStatusDomain_ae217ea8-d0bb-4eea-a27b-438a1bbe3fc8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PendingLitigationMember_47631312-b27c-4199-bcd8-01cbbfa10a81" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PendingLitigationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LitigationStatusDomain_ae217ea8-d0bb-4eea-a27b-438a1bbe3fc8" xlink:to="loc_us-gaap_PendingLitigationMember_47631312-b27c-4199-bcd8-01cbbfa10a81" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AcquisitionRestructuringandOtherItemsNet" xlink:type="simple" xlink:href="ango-20200831.xsd#AcquisitionRestructuringandOtherItemsNet"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AcquisitionRestructuringandOtherItemsNet" xlink:type="extended" id="i1e75247cecd14facbec0567f603e6040_AcquisitionRestructuringandOtherItemsNet"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AcquisitionRestructuringandOtherItemsNetTables" xlink:type="simple" xlink:href="ango-20200831.xsd#AcquisitionRestructuringandOtherItemsNetTables"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AcquisitionRestructuringandOtherItemsNetTables" xlink:type="extended" id="ib757f0f3ba094a55b7023b5a4c32026b_AcquisitionRestructuringandOtherItemsNetTables"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AcquisitionRestructuringandOtherItemsNetSummaryDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#AcquisitionRestructuringandOtherItemsNetSummaryDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AcquisitionRestructuringandOtherItemsNetSummaryDetail" xlink:type="extended" id="ib06c0c2679924f4eace03dd65db5e490_AcquisitionRestructuringandOtherItemsNetSummaryDetail">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringCostAndReserveLineItems_af411f54-f12a-4e94-9ff0-a541da2f8bad" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestructuringCostAndReserveLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_AcquisitionRestructuringAndOtherItemsNet_8c03aa9f-caa5-4045-92c9-9a6f59a8199b" xlink:href="ango-20200831.xsd#ango_AcquisitionRestructuringAndOtherItemsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_af411f54-f12a-4e94-9ff0-a541da2f8bad" xlink:to="loc_ango_AcquisitionRestructuringAndOtherItemsNet_8c03aa9f-caa5-4045-92c9-9a6f59a8199b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_2785c617-77d7-4745-aed8-2b6e17f34b5e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfRestructuringAndRelatedCostsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_af411f54-f12a-4e94-9ff0-a541da2f8bad" xlink:to="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_2785c617-77d7-4745-aed8-2b6e17f34b5e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_8de9dc41-133f-489a-a204-0243f0085b30" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_2785c617-77d7-4745-aed8-2b6e17f34b5e" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_8de9dc41-133f-489a-a204-0243f0085b30" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_8de9dc41-133f-489a-a204-0243f0085b30_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_8de9dc41-133f-489a-a204-0243f0085b30" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_8de9dc41-133f-489a-a204-0243f0085b30_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_86b23938-36a1-4e36-bd03-3def410ae2d1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_8de9dc41-133f-489a-a204-0243f0085b30" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_86b23938-36a1-4e36-bd03-3def410ae2d1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_LegalCostsMember_f8df4dcd-faf3-4df0-b2a7-1c5787fdb2b0" xlink:href="ango-20200831.xsd#ango_LegalCostsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_86b23938-36a1-4e36-bd03-3def410ae2d1" xlink:to="loc_ango_LegalCostsMember_f8df4dcd-faf3-4df0-b2a7-1c5787fdb2b0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_MergersandAcquisitionsMember_4d9df503-61a5-4feb-aa0a-8ed58d402d78" xlink:href="ango-20200831.xsd#ango_MergersandAcquisitionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_86b23938-36a1-4e36-bd03-3def410ae2d1" xlink:to="loc_ango_MergersandAcquisitionsMember_4d9df503-61a5-4feb-aa0a-8ed58d402d78" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_TransitionserviceagreementMember_40ccdd3f-c8f3-4ade-8727-04d25ae31996" xlink:href="ango-20200831.xsd#ango_TransitionserviceagreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_86b23938-36a1-4e36-bd03-3def410ae2d1" xlink:to="loc_ango_TransitionserviceagreementMember_40ccdd3f-c8f3-4ade-8727-04d25ae31996" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_DivestitureMember_929fcd1a-bef5-42cb-b300-36cef05f436a" xlink:href="ango-20200831.xsd#ango_DivestitureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_86b23938-36a1-4e36-bd03-3def410ae2d1" xlink:to="loc_ango_DivestitureMember_929fcd1a-bef5-42cb-b300-36cef05f436a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringChargesMember_afe6062c-81b2-422f-97c1-e5e1d6b67778" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestructuringChargesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_86b23938-36a1-4e36-bd03-3def410ae2d1" xlink:to="loc_us-gaap_RestructuringChargesMember_afe6062c-81b2-422f-97c1-e5e1d6b67778" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherExpenseMember_cc3de04f-7773-49b5-aa50-0a0235131ac2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_86b23938-36a1-4e36-bd03-3def410ae2d1" xlink:to="loc_us-gaap_OtherExpenseMember_cc3de04f-7773-49b5-aa50-0a0235131ac2" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AcquisitionRestructuringandOtherItemsNetNarrativeDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#AcquisitionRestructuringandOtherItemsNetNarrativeDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AcquisitionRestructuringandOtherItemsNetNarrativeDetail" xlink:type="extended" id="i5da22f896886450b80a9e872675d1ae0_AcquisitionRestructuringandOtherItemsNetNarrativeDetail">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringCostAndReserveLineItems_906525ad-9a85-4c58-b463-9eb26361aee3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestructuringCostAndReserveLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_AcquisitionRestructuringAndOtherItemsNet_42cf8943-40e0-4206-be26-50d61482dfea" xlink:href="ango-20200831.xsd#ango_AcquisitionRestructuringAndOtherItemsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_906525ad-9a85-4c58-b463-9eb26361aee3" xlink:to="loc_ango_AcquisitionRestructuringAndOtherItemsNet_42cf8943-40e0-4206-be26-50d61482dfea" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromLegalSettlements_81d84505-fb32-4390-9743-b56711ad1b0d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ProceedsFromLegalSettlements"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_906525ad-9a85-4c58-b463-9eb26361aee3" xlink:to="loc_us-gaap_ProceedsFromLegalSettlements_81d84505-fb32-4390-9743-b56711ad1b0d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_24dfbc86-e7e5-47d9-930d-0dc9106be34e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfRestructuringAndRelatedCostsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_906525ad-9a85-4c58-b463-9eb26361aee3" xlink:to="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_24dfbc86-e7e5-47d9-930d-0dc9106be34e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_LitigationCaseAxis_a8f97d3e-a3ca-46ff-9216-9794cf6ba6df" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_LitigationCaseAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_24dfbc86-e7e5-47d9-930d-0dc9106be34e" xlink:to="loc_srt_LitigationCaseAxis_a8f97d3e-a3ca-46ff-9216-9794cf6ba6df" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_LitigationCaseTypeDomain_a8f97d3e-a3ca-46ff-9216-9794cf6ba6df_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_LitigationCaseTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_LitigationCaseAxis_a8f97d3e-a3ca-46ff-9216-9794cf6ba6df" xlink:to="loc_srt_LitigationCaseTypeDomain_a8f97d3e-a3ca-46ff-9216-9794cf6ba6df_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_LitigationCaseTypeDomain_6a7c90ac-c9ba-4276-b20f-9ab1aec13fb6" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_LitigationCaseTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_LitigationCaseAxis_a8f97d3e-a3ca-46ff-9216-9794cf6ba6df" xlink:to="loc_srt_LitigationCaseTypeDomain_6a7c90ac-c9ba-4276-b20f-9ab1aec13fb6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_BiolitecBankruptcyMember_da9b8613-9c17-4fb7-a1ac-d66ba23bec32" xlink:href="ango-20200831.xsd#ango_BiolitecBankruptcyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_LitigationCaseTypeDomain_6a7c90ac-c9ba-4276-b20f-9ab1aec13fb6" xlink:to="loc_ango_BiolitecBankruptcyMember_da9b8613-9c17-4fb7-a1ac-d66ba23bec32" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_362388a2-d1ed-4175-87c5-a09d1140184e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_24dfbc86-e7e5-47d9-930d-0dc9106be34e" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_362388a2-d1ed-4175-87c5-a09d1140184e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_362388a2-d1ed-4175-87c5-a09d1140184e_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_362388a2-d1ed-4175-87c5-a09d1140184e" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_362388a2-d1ed-4175-87c5-a09d1140184e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_1e0f52a2-44d0-4b65-a7f7-50814c515eae" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_362388a2-d1ed-4175-87c5-a09d1140184e" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_1e0f52a2-44d0-4b65-a7f7-50814c515eae" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ango_LegalCostsMember_df39c568-0ce1-40c1-81f0-dc1f24485524" xlink:href="ango-20200831.xsd#ango_LegalCostsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_1e0f52a2-44d0-4b65-a7f7-50814c515eae" xlink:to="loc_ango_LegalCostsMember_df39c568-0ce1-40c1-81f0-dc1f24485524" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AccumulatedOtherComprehensiveIncomeLoss" xlink:type="simple" xlink:href="ango-20200831.xsd#AccumulatedOtherComprehensiveIncomeLoss"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AccumulatedOtherComprehensiveIncomeLoss" xlink:type="extended" id="id495b884219449228ca27e79522b96da_AccumulatedOtherComprehensiveIncomeLoss"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AccumulatedOtherComprehensiveIncomeLossTables" xlink:type="simple" xlink:href="ango-20200831.xsd#AccumulatedOtherComprehensiveIncomeLossTables"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AccumulatedOtherComprehensiveIncomeLossTables" xlink:type="extended" id="id6408c23b90e4795b1726085d1a5f797_AccumulatedOtherComprehensiveIncomeLossTables"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/AccumulatedOtherComprehensiveIncomeLossScheduleDetail" xlink:type="simple" xlink:href="ango-20200831.xsd#AccumulatedOtherComprehensiveIncomeLossScheduleDetail"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/AccumulatedOtherComprehensiveIncomeLossScheduleDetail" xlink:type="extended" id="i69f5d71b58a6422bb25ebdf616c2e33f_AccumulatedOtherComprehensiveIncomeLossScheduleDetail">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_fada78f2-a438-4d4f-acdc-4b30d37eefb5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_c34464d6-21fa-40f6-84ff-3912a68eea92" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_fada78f2-a438-4d4f-acdc-4b30d37eefb5" xlink:to="loc_us-gaap_StockholdersEquity_c34464d6-21fa-40f6-84ff-3912a68eea92" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OciBeforeReclassificationsBeforeTaxAttributableToParent_d980b7b6-8864-48dd-887e-4e0aa6804c4e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OciBeforeReclassificationsBeforeTaxAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_fada78f2-a438-4d4f-acdc-4b30d37eefb5" xlink:to="loc_us-gaap_OciBeforeReclassificationsBeforeTaxAttributableToParent_d980b7b6-8864-48dd-887e-4e0aa6804c4e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationFromAociCurrentPeriodNetOfTaxAttributableToParent_649c484b-c1f9-4211-951f-961bae045a24" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ReclassificationFromAociCurrentPeriodNetOfTaxAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_fada78f2-a438-4d4f-acdc-4b30d37eefb5" xlink:to="loc_us-gaap_ReclassificationFromAociCurrentPeriodNetOfTaxAttributableToParent_649c484b-c1f9-4211-951f-961bae045a24" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_078e6b3e-6103-4024-84f3-c50edb9daab2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_fada78f2-a438-4d4f-acdc-4b30d37eefb5" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_078e6b3e-6103-4024-84f3-c50edb9daab2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_32f5b556-8c3a-4238-9d39-4edda982fcf6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_c0d27a6f-3850-4fea-b629-1845cbf52778" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_fada78f2-a438-4d4f-acdc-4b30d37eefb5" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_c0d27a6f-3850-4fea-b629-1845cbf52778" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_e48f9b1f-41ac-4aa1-bd0e-ad5d9031bc04" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_c0d27a6f-3850-4fea-b629-1845cbf52778" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_e48f9b1f-41ac-4aa1-bd0e-ad5d9031bc04" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_e48f9b1f-41ac-4aa1-bd0e-ad5d9031bc04_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_e48f9b1f-41ac-4aa1-bd0e-ad5d9031bc04" xlink:to="loc_us-gaap_EquityComponentDomain_e48f9b1f-41ac-4aa1-bd0e-ad5d9031bc04_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_180033d6-33f0-4327-84db-ea0032cf7e2e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_e48f9b1f-41ac-4aa1-bd0e-ad5d9031bc04" xlink:to="loc_us-gaap_EquityComponentDomain_180033d6-33f0-4327-84db-ea0032cf7e2e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_14e4f49a-499e-4c68-a2e0-aae38839d1cd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_180033d6-33f0-4327-84db-ea0032cf7e2e" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_14e4f49a-499e-4c68-a2e0-aae38839d1cd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedTranslationAdjustmentMember_8836d62b-48f5-41af-9b35-6ee5f917fd63" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedTranslationAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_14e4f49a-499e-4c68-a2e0-aae38839d1cd" xlink:to="loc_us-gaap_AccumulatedTranslationAdjustmentMember_8836d62b-48f5-41af-9b35-6ee5f917fd63" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/RecentlyIssuedAccountingPronouncements" xlink:type="simple" xlink:href="ango-20200831.xsd#RecentlyIssuedAccountingPronouncements"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/RecentlyIssuedAccountingPronouncements" xlink:type="extended" id="ia8ae0df8647a4e86b4aad132f87628b3_RecentlyIssuedAccountingPronouncements"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/RecentlyIssuedAccountingPronouncementsPolicies" xlink:type="simple" xlink:href="ango-20200831.xsd#RecentlyIssuedAccountingPronouncementsPolicies"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/RecentlyIssuedAccountingPronouncementsPolicies" xlink:type="extended" id="i8b84283fe92f4ad3b2572cb829cfbc48_RecentlyIssuedAccountingPronouncementsPolicies"/>
  <link:roleRef roleURI="http://www.angiodynamics.com/role/RecentlyIssuedAccountingPronouncementsTables" xlink:type="simple" xlink:href="ango-20200831.xsd#RecentlyIssuedAccountingPronouncementsTables"/>
  <link:definitionLink xlink:role="http://www.angiodynamics.com/role/RecentlyIssuedAccountingPronouncementsTables" xlink:type="extended" id="i98afa3c6bffd4a4eab0db404f3713a4f_RecentlyIssuedAccountingPronouncementsTables"/>
</link:linkbase>
